Non-Invasive Biochemical Methods for Assessing Human Embryo Quality
While human in vitro fertilisation (IVF) and Embryo Transfer techniques have helped overcome the problems of infertility in some thousands of couples, success rates remain disappointingly low. The major problem remains the embryo transfer stage where only a comparatively small proportion of embryos implant and are carried to term successfully. Embryos for transfer are assessed on the basis of their morphology and extent of development in culture. These methods are notoriously imprecise and there is a need for quantitative non-invasive assays of human embryo quality.
KeywordsGlucose Uptake Human Embryo Blastocyst Stage Preimplantation Embryo Noninvasive Measurement
Unable to display preview. Download preview PDF.
- Gardner, D.K., and Leese, H.J., 1986, Non-invasive measurement of nutrient uptake by single cultured preimplantation mouse embryos, Human Reprod., 1:25.Google Scholar
- Leese, H.J., and Gardner, D.K., 1989, Embryo metabolism and viability in vitro, in: “CRC Handbook of IVF and ET: Laboratory Manual,” A. Trounson and J. Osborn, eds., CRC Press Inc., U.S.A.Google Scholar
- Renard, J.P., Philipon, A., and Memezo, Y., In vitro uptake of glucose by bovine blastocysts, J. Reprod. Fert., 58:161.Google Scholar
- Tesarik, J., 1987, Gene activation in the human embryo developing in vitro, in: “Future Aspects in Human In Vitro Fertilization,” W. Feichtinger, and P Kemeter, eds., Springer-Verlag, Berlin and Heidelberg.Google Scholar
- Wales, R.G., Whittingham, R.G., Hardy, K., and Craft, I.L., Metabolism of glucose by human embryos, J. Reprod. Fert., 79:289.Google Scholar