Immunologic Methods for the Detection of Carcinogen Adducts in Humans
Immunologic methods are now available for the sensitive detection and quantitation of carcinogen-DNA adducts. Monoclonal and polyclonal antibodies have been developed against a number of specific adducts as well as against UV-damaged DNA (Poirier, 1981; Santella, 1988). Antibodies can be developed against either the carcinogen adduct covalently coupled to carrier protein or the modified DNA electrostatically complexed to methylated bovine serum albumin. These antibodies can be used in highly sensitive competitive enzyme-linked immunosorbent assays (ELISA) with color- or fluorescence endpoint detection. Since femtomole (10-15) sensitivities are readily attainable, DNA adduct levels in the range of 1/108 nucleotides can be measured. With monoadduct-specific antibodies, higher sensitivities may be obtainable if large amounts of DNA are available and the adduct is isolated by various chromatographic procedures before quantitation in the ELISA. Table 1 lists the monoclonal antibodies recognizing carcinogen-DNA adducts which we have developed to date. Several of these antibodies, including those recognizing aflatoxin, benzo(a)pyrene diol epoxide and 8-methoxypsoralen-DNA adducts, have been applied to adduct detection in humans.
KeywordsPAHs Adduct Psoriasis Pyrene Fluorescein
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