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Immunologic Methods for the Detection of Carcinogen Adducts in Humans

  • Regina M. Santella
  • Xiao Yen Yang
  • Ling Ling Hsieh
  • Tie Lan Young
  • Xiao Qing Lu
  • Marina Stefanidis
  • Frederica P. Perera
Part of the Basic Life Sciences book series (BLSC, volume 53)

Abstract

Immunologic methods are now available for the sensitive detection and quantitation of carcinogen-DNA adducts. Monoclonal and polyclonal antibodies have been developed against a number of specific adducts as well as against UV-damaged DNA (Poirier, 1981; Santella, 1988). Antibodies can be developed against either the carcinogen adduct covalently coupled to carrier protein or the modified DNA electrostatically complexed to methylated bovine serum albumin. These antibodies can be used in highly sensitive competitive enzyme-linked immunosorbent assays (ELISA) with color- or fluorescence endpoint detection. Since femtomole (10-15) sensitivities are readily attainable, DNA adduct levels in the range of 1/108 nucleotides can be measured. With monoadduct-specific antibodies, higher sensitivities may be obtainable if large amounts of DNA are available and the adduct is isolated by various chromatographic procedures before quantitation in the ELISA. Table 1 lists the monoclonal antibodies recognizing carcinogen-DNA adducts which we have developed to date. Several of these antibodies, including those recognizing aflatoxin, benzo(a)pyrene diol epoxide and 8-methoxypsoralen-DNA adducts, have been applied to adduct detection in humans.

Keywords

Psoriasis Patient Competitive ELISA Adduct Level Skin Irradiation Aryl Hydrocarbon Hydroxylase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Regina M. Santella
    • 1
  • Xiao Yen Yang
    • 1
  • Ling Ling Hsieh
    • 1
  • Tie Lan Young
    • 1
  • Xiao Qing Lu
    • 1
  • Marina Stefanidis
    • 1
  • Frederica P. Perera
    • 1
  1. 1.Cancer Center and Division of Environmental Science, School of Public HealthColumbia UniversityNew YorkUSA

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