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Measurements of Genomic and Gene-Specific DNA Repair of Alkylation Damage in Cultured Human T-Lymphocytes

  • Jeanette N. Hartshorn
  • David A. Scicchitano
  • Steven H. Robison
Part of the Basic Life Sciences book series (BLSC, volume 53)

Abstract

Due to the predominance of mutagens and carcinogens in the environment and the prevalance of cancers in the human population, there exists a need to understand the mechanism and efficiency of DNA repair in human cells. Most of the mechanisms which have been proposed for the repair of various types of damage in human cells has been based upon prokaryotic models (Friedberg, 1985); however, our knowledge of DNA repair processes in human cells is not as extensive as that of prokaryotic cells. Unlike bacterial systems, there have been no readily available repair deficient mutants in which to study DNA repair mechanisms until recently. In the past, lymphoblast and fibroblast cell lines have been established from individuals with disorders which have been attributed to a defect in or alteration of DNA repair and predispositions to cancer (such as xeroderma pigmentosum, ataxia telangiectasia, and Fanconi’s anemia) in order to dissect the mechanisms of DNA repair. These cell lines have been instrumental in the characterization of the human DNA repair genes and have permitted the assignment of two human DNA repair genes to chromosome 19, ERCC I and ERCC II involved in excision repair (van Duin et al., 1986; Weber et al., 1988).

Keywords

Ataxia Telangiectasia Xeroderma Pigmentosum Bloom Syndrome Dhfr Gene Alkaline Elution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Jeanette N. Hartshorn
    • 1
  • David A. Scicchitano
    • 2
  • Steven H. Robison
    • 1
  1. 1.VRCC-Genetics LaboratoryBurlingtonUSA
  2. 2.Division of Toxicology and PathologyAmerican Health FoundationValhallaUSA

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