Non-Culture Tests for the Diagnosis of Gonorrhea

  • Leonard Zubrzycki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 263)

Abstract

Gram-stain examination and culture of clinical specimens are the standard diagnostic tests for gonorrhea. However, both techniques have limitations. Gram stain is relatively insensitive for the diagnosis of cervical and rectal infections, and correct Interpretation of the Gram stain requires an experienced reader.1 Culture results may be unsatisfactory, unless selective culture medium is quality controlled, stored, and Inoculated properly, and promptly Incubated in a CO2-enriched atmosphere. Also the culture method using the common selective medium fails to detect vancomycin-susceptible strains of Neisseria gonorrhoeae. Further problems arise when specimens must be sent to another facility for culturing.

Keywords

Cellulose Recombination Iodide Expense Vancomycin 

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Literature Cited

  1. 1.
    M. E. Goodhart, J. Ogden, A. A. Zaidi and S. T. Kraus, Factors affecting the performance of smear and culture tests for the detection of Neisseria gonorrhoeae Sex. Trans. Dis. 9:63 (1982).Google Scholar
  2. 2.
    T. J. Vecchio, Predictive value of a single diagnostic test in unselected populations, New Eng. J. Med. 274:1171 (1966).Google Scholar
  3. 3.
    J. R. Koransky and N. F. Jacobs, Serologic testing for gonorrhea, Sex. Trans. Dis. 4:27 (1977).Google Scholar
  4. 4.
    E. A. Donegan, Serological tests to diagnose gonococcal infections, in: “Gonococcal Infection,” G. F. Brooks and E. A. Donegan, eds., Edward Arnold, London (1985).Google Scholar
  5. 5.
    R. B. Prior and V. A. Spagna, Improved utility of gonoscreen, a Limuius amoebocyte lysate assay, In the evaluation of urethral discharges in men, J. Clin. Microbiol. 22:141 (1985).PubMedGoogle Scholar
  6. 6.
    F. N. Judson, B. A. Werness and M. R. Shahan, Lack of utility of a limulus amoebocyte lysate assay in the diagnosis of urethral discharges in men, J. Clin. Microbiol. 21:152 (1985).PubMedGoogle Scholar
  7. 7.
    Y. M. Felman and D. C. William, Gonodecten - new 3-minute in vitro diagnostic test for gonorrhea in the male without use of conventional culture or gram stain, Urology Vol. XIX (1982).Google Scholar
  8. 8.
    W. M. Janda and T. Jackson, Evaluation of Gonodecten for the presumptive diagnosis of gonococcal urethritis in men, J. Clin. Microbiol. 21:143 (1985).PubMedGoogle Scholar
  9. 9.
    M. M. Takeguchi, H. H. Weetal, D. K. Smith, H. C. McDonald, K. A. Livsey, C. C. Detar and T. A. Chapel, Enzymatic detection of Neisseria gonorrhoeae Br. J. Vener. Dis. 56:304 (1980).PubMedGoogle Scholar
  10. 10.
    M. J. Thorniey, D. V. Wilson, R. Demarco De Hormaeche, J. K. Oates and R. R. A. Coombs, Detection of gonococcal antigens in urine by radloimmunotogyassay, J. Med. Mlcrobioi. 12:161 (1979).CrossRefGoogle Scholar
  11. 11.
    I. J. Sud and D. S. Feingold, Detection of 3-hydroxy fatty acids at picogram levels in biological specimens. A chemical method for the detection of Neisseria gonorrhoeae7 J. Invest. Dermatol. 73:521 (1979).PubMedCrossRefGoogle Scholar
  12. 12.
    H. Young, S. K. Saraflan, A. B. Harris and A. McMillan, A non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings, J. Med. Mlcrobioi. 16:183 (1983).CrossRefGoogle Scholar
  13. 13.
    W. E. Stamm, B. Cole, C. Fennell, P. Bonin, A. S. Armstrong, J. E. Herrmann and K. K. Holmes, Antigen detection for the detection of gonorrhea, J. Clin. Microbiol. 19:399 (1984).PubMedGoogle Scholar
  14. 14.
    C. J. Papasian, W. R. Bartholomew and D. Amsterdam, Validity of an enzyme immunoassay for detection of Neisseria gonorrhoeae antigens, J. Clin. Microbiol. 19:347 (1984).PubMedGoogle Scholar
  15. 15.
    J. Schachter, W. M. McCormack, R. F. Smith, R. M. Parks, R. Bailey and A. C. Ohlin, Enzyme immunoassay for diagnosis of gonorrhea, J. Clin. Microbiol. 19:57 (1984).PubMedGoogle Scholar
  16. 16.
    M. A. Nasello, D. R. Callihan, M. A. Menegus and R. T. Steigibel, A solid-phase enzyme immunoassay (Gonozyme) test for direct detection of Neisseria gonorrhoeae antigen in urogenital specimens from patients at a sexually transmitted disease clinic, Sex. Trans. Dis. 12:198 (1985).Google Scholar
  17. 17.
    P. A. Granato and M. Roefaro, Comparative evaluation of enzyme immunoassay and culture for the laboratory diagnosis of gonorrhea, Am. J. Clin. Pathol. 83:613 (1985).PubMedGoogle Scholar
  18. 18.
    I. Nachamkin, S. J. Sondheimer, S. Barbargallo and S. Barth, Detection of Neisseria gonorrhoeae In cervical swabs using the Gonozyme enzyme immunoassay, Am. J. Clin. Pathol. 82:461 (1984).PubMedGoogle Scholar
  19. 19.
    J. T. Rudrik, J. M. Waller and E. M. Britt, Efficacy of an enzyme immunoassay with ultracentrifuged first-voided urine for detection of gonorrhea in males, J. Clin. Microbiol. 20:577 (1984).PubMedGoogle Scholar
  20. 20.
    J. Schachter, F. Pang, R. M. Parks, R. F. Smith and A. S. Armstrong, Use of gonozyme on urine sediment for diagnosis of gonorrhea in males, J. Clin. Microbiol. 23:124 (1986).PubMedGoogle Scholar
  21. 21.
    P. A. Totten, K. K. Holmes, H. H. Handsfield, J. S. Knapp, P. L. Perine and S. Faikow, DNA hybridization technique for the detection of Neisseria gonorrhoeae in men with urethritis, J. Inf. Dis. 148:462 (1983).CrossRefGoogle Scholar
  22. 22.
    P. L. Perine, P. A. Totten, K. K. Holmes, E. H. Sng, A. V. Ratnam, R. Widy-Wersky, H. Nsanze, E. Habte-Gabr and W. G. Westbrook, Evaluation of a DNA-hybridization method for detection of African and Asian strains of Neisserla gonorrhoeae in men with urethritis, J. Inf. Dis. 152:59 (1985).CrossRefGoogle Scholar
  23. 23.
    A. Janik, E. Juni and G. A. Heyn, Genetic transformation as a tool for detection of Neisseria gonorrhoeae J. Clin. Microblol. 4:71 (1976).Google Scholar
  24. 24.
    H. W. Jaffe, S. J. Kraus, T. A. Edwards, S. S. Weinberger and L. Zubrzyckl, Diagnosis of gonorrhea using a genetic transformation test on mailed clinical specimens, J. Inf. Dis. 146:275 (1982).CrossRefGoogle Scholar
  25. 25.
    W. L. Whittington, M. Miller, J. Lewis, J. Parker, J. Biddle and L. Zubrzycki, Diagnosis of gonococcal infection: A genetic transformation test performed on specimens mailed to a clinical laboratory, Sex. Trans. Dis., submitted (1987).Google Scholar
  26. 26.
    Centers for Disease Control, “Antibiotic-Resistant Strains of Neisseria gonorrhoeae,” MMWR (suppl. 5S), vol. 36 (1987).Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Leonard Zubrzycki
    • 1
  1. 1.Department of Microbiology and ImmunologyTemple University School of MedicinePhiladelphiaUSA

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