Carbonic Anhydrase Activity of Primary Afferent Neurons in Rat: Attempt at Marking Functionally Related Subpopulations

  • M. Szabolcs
  • M. Kopp
  • G. Schaden


Earlier studies have described strong or moderate activities of carbonic anhydrase (CA; E.C. in primary afferent neurons with axons of intermediate or large size.1-5 Enzyme activity of the individual neuron, however, remains constant throughout the entire length of its axis cylinder,6 which greatly facilitates tracing of neurons with different carbonic anhydrase activities. This phenomenon led to the observation4,6–8 that the spinal projection of the central processes of highly CA-positive dorsal root ganglion (DRG) cells corresponds well to the projection of type I muscle afferents. The assumption that the highly CA-reactive neurons are proprioceptive is corroborated by the fact that anulo-spiral nerve terminals enwrapping intrafusal muscle fiber are heavily stained.8 The present study is an attempt at assigning such CA-reactive afferent nerve fibers to a functionally related subpopulation.1,4,5


Nerve Fiber Dorsal Root Ganglion Carbonic Anhydrase Muscle Spindle Primary Afferent Neuron 
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  1. 1.
    W. Crammer, R. Sacchi, and V. Sapirstein, Immunocytochemical localization of carbonic anhydrase in the spinal cord of normal and mutant (shiverer) adult mice with comparison among fixation methods, J. Histochem. Cytochem.33:45 (1985).CrossRefGoogle Scholar
  2. 2.
    B. Droz, and J. Kazimierczak, Carbonic anhydrase in primary-sensory neurons of dorsal root ganglia, Comp. Biochem. Physiol.88B: 713 (1987).Google Scholar
  3. 3.
    J. Kazimierczak, E. W. Sommer, E. Philippe, and B. Droz, Carbonic anhydrase activity in primary sensory neurons — I. Requirements for the cytochemical localization in the dorsal root ganglion of chicken and mouse by light and electron microscopy, Cell Tissue Res. 245:487 (1986).PubMedCrossRefGoogle Scholar
  4. 4.
    D. A. Riley, S. Ellis, and J. Bain, Carbonic anhydrase activity in skeletal muscle fibre types, axons, spindles, and capillaries of the rat soleus and extensor digitorum longus muscles, J. Histochem. Cytochem.30:1275 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    V. Wong, C. P. Barrett, E. J. Donati, and L. Guth, Distribution of carbonic anhydrase activity in neurons of the rat, J. Comp. Neurol.257:122 (1987).PubMedCrossRefGoogle Scholar
  6. 6.
    V. Wong, C. P. Barrett, E. J. Donati, L. F. Eng, and L. Guth, Carbonic anhydrase activity in the first order sensory neuron of the rat, J. Histochem. Cytochem., 31:293 (1983).PubMedCrossRefGoogle Scholar
  7. 7.
    J. M. Peyronnard, J. P. Messier, L. Charron, J. Lavoie, F. X. Bergouignan, and M. Dubreuil, Carbonic anhydrase activity in the normal and injured peripheral nervous system of the rat, Exp. Neurol.93:481 (1986).PubMedCrossRefGoogle Scholar
  8. 8.
    D. A. Riley, S. Ellis, and J. L. W. Bain, Ultrastructural cytochemical localization of carbonic anhydrase activity in rat peripheral sensory and motor nerves, dorsal root ganglia and column nuclei, Neuroscience13:189 (1984).PubMedCrossRefGoogle Scholar
  9. 9.
    J. Gottschall, W. Zenker, W. Neuhuber, A. Mysicka, and M. Müntener, The sternomastoid muscle of rat and its innervation. Muscle fiber composition; perikarya and axons of efferent and afferent neurons, Anat. Embryol.160:285 (1980).PubMedCrossRefGoogle Scholar
  10. 10.
    E. B. Krammer, M. F. Lischka, T. P. Egger, M. Riedl, and H. Gruber, The motoneuronal organisation of the spinal accessory nuclear complex. Adv. Anat. Embryol.103:1 (1987).Google Scholar
  11. 11.
    H. P. J. Hansson, Histochemical demonstration of carbonic anhydrase activity, Histochemie11:112 (1967).PubMedCrossRefGoogle Scholar
  12. 12.
    G. Lönnerholm, Carbonic anhydrase in rat liver, rabbit skeletal muscle; further evidence for the specifity of the histochemical cobalt-phosphate method of Hansson, J. Histochem. Cytochem.28:427 (1980).PubMedCrossRefGoogle Scholar
  13. 13.
    S. Rosen, and G. L. Musser, Observations on the specifity of newer histochemical methods for the demonstration of carbonic anhydrase activity, J. Histochem. Cytochem.20:951 (1972).PubMedCrossRefGoogle Scholar
  14. 14.
    C. C. Hunt, Relation of function to diameter in afferent fibres of muscle nerves, J. Gen. Physiol.38:117 (1954).PubMedCrossRefGoogle Scholar
  15. 15.
    D. P. C. Lloyd, Conduction and synaptic transmission of reflex responses to stretch in spinal cats, J. Neurophysiol. 6:317 (1943).Google Scholar
  16. 16.
    R. E. Burke, Firing patterns of gastrocnemius motor units in the decerebrate cat, J. Physiol. (Lond.)196:631 (1968).Google Scholar
  17. 17.
    T. W. Schoultz, and J. E. Swett, The fine structure of the Golgi tendon organ, J. Neurocytol.1:1 (1972).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • M. Szabolcs
    • 1
  • M. Kopp
    • 1
  • G. Schaden
    • 1
  1. 1.Anatomisches InstitutUniversität WienWienAustria

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