Parathyroid Hormone and Phosphatidylinositol Metabolism in Fetal Rat Limb Bones

  • Paula H. Stern
  • Victoria M. Stathopoulos


We have previously shown that bPTH-(l-34) stimulates the incorporation of 3H-myo-inositol into phosphatidylinositol in fetal rat limb bones (Rappaport and Stern, 1986). The current studies were undertaken to determine whether the hormone would also show effects on the production of inositol phosphates. We have compared the effects of bPTH-(l-34) with those of Nleu8,18Tyr34-bPTH-(3-34)amide, a PTH analog shown to be without effects on cyclic AMP in renal (Rosenblatt, et al., 1977) or osseous (Goldring, et al., 1985, Hermann-Erlee, et al., 1983) tissue. In addition, we have examined the effects of α-thrombin, which stimulates resorption in fetal rat limb bones (Hoffmann, et al., 1986) and which increases production of inositol phosphates in several tissues (Rendu et al., 1983, Carney, et al. 1985).


Parathyroid Hormone Osteosarcoma Cell Inositol Phosphate Phospholipid Metabolism Inositol Trisphosphate 
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  1. Bidot-Lopez, P., Farese, R. V., Sibir, M. A., 1981, Parathyroid hor-mone and adenosine-3’ ,5’-monophosphate acutely increase phospholipids of the phosphatidate-polyphosphoinositide pathway in rabbit kidney cortex tubules in vitro by a cycloheximide-sensitive process, Endocrinology, 108: 2078.Google Scholar
  2. Boland, C. J., Fried. R. M., Tashjian, A. H., Jr.,1986, Measurement of cytosolic free Ca2+ concentrations in human and rat osteosarcoma cells: actions of bone resorption-stimulating hormones, Endocrinology, 118:980.Google Scholar
  3. Carney, D. H., Scott, D. L., Gordon, E. A., LaBelle, E. F., 1985, Phosphoinositides in mitogenesis: neomycin inhibits thrombin-stimulated phosphoinositide turnover and initiation of cell proliferation. Cell42: 479.Google Scholar
  4. Dziak, R., Stern, P. H. 1975, Calcium transport in isolated bone cells. III. Effects of parathyroid hormone and cyclic 3’,5’-AMP, Endocrinology.97: 1281.Google Scholar
  5. Goldring, S. R., Roelke, M. S., Bringhurst, F. R., Rosenblatt, M., 1985, Differential effects of parathyroid hormone responsive cultured human cells on biological activity of parathyroid hormone and parathyroid hormone inhibitory analogs. Biochemistry.24: 513.Google Scholar
  6. Hoffmann, 0., Klaushofer, K., Koller, K., Peterlik, M. Mavreas, T., Stern, P., 1986, Indomethacin inhibits thrombin-, but not thyroxin-stimulated resorption of fetal rat limb bones, Prostaglandins31: 601.Google Scholar
  7. Lerner, U. H., Dewhirst, F. E., Ransjo, M., Sahlberg, K., and Fredholm, B. B., 1987, On the role of cyclic AMP and inositol phosphates as second messenger of the bone resorptive effect of parathyroid hormone, lα(OH) vitamin D3 and interleukin 1, in: ″Calcium Regulation and Bone Metabolism: Basic and Clinical Aspects,″ vol. 9 D. V., Cohn, T. J. Martin, P. J. Meunier, Elsevier, Amsterdam.Google Scholar
  8. Lorenzo, J., Raisz, L. G., 1981, Divalent cation ionophores stimulate resorption and inhibit DNA synthesis in cultured fetal rat bone, Science212: 1157.Google Scholar
  9. Lowik, C. W. G. M., van Leeuwen, J. P. T. M., van der Meer, J. M., van Zeeland, J. K., Scheven, B. A. A., Herrmann-Erlee, M. P. M., 1985, A two-receptor model for the action of parathyroid hormone on osteoblasts: a role for intracellular free calcium and cAMP, Cell Calcium,6: 311.Google Scholar
  10. Matsumoto, T., Morita, K., Kawanobe, Y., Ogata, E., 1986, Effect of parathyroid hormone on phospholipid metabolism in osteoblast-like osteog-enic sarcoma cells, Biochem J.236: 605.Google Scholar
  11. Parsons, J. A., Neer, R. M., Potts, J. T., Jr., 1971, Initial fall of plasma calcium after intravenous injection of parathyroid hormone, Endocrinology.89: 735.Google Scholar
  12. Raisz, L. G., Trummel, C. L., and Simmons, H., 1972, Induction of bone resorption in tissue culture: prolonged response after brief exposure to parathyroid hormone or 25-hydroxycholecalciferol, Endocrinology.90: 744.PubMedGoogle Scholar
  13. Stern, P. H., 1984, Role of calcium in the initiation of hormonal effects on bone, in: Epithelial Calcium and Phosphate Transport: Molecular and Cellular Aspects, M. Peterlik and F. Bronner, eds. Alan R. Liss, Inc. New York, p. 159.Google Scholar
  14. Stern, P. H. 1985, Interactions of calcemic hormones and divalent cation ionophores on fetal rat bone in vitro, in: Calcium in Biological Systems, R. P. Rubin, G. B. Weiss, J. W. Putney, Jr., eds. Plenum, New York, p. 541.Google Scholar
  15. Wakelam, M. J. 0., Murphy, G. J., Hruby, V. J., and Houslay, M. D., 1986, Activation of two signal-transduction systems in hepatocytes by glucagon, Nature, 323: 68.Google Scholar
  16. Wong, G. L., Kent, G. N., Ku, K. Y., Cohn, D. V., 1978, The interaction of parathormone and calcium on the hormone-regulated synthesis of hyaluronic acid and citrate decarboxylation in isolated bone cells, Endo-crinology.103: 2274.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Paula H. Stern
    • 1
  • Victoria M. Stathopoulos
    • 1
  1. 1.Department of PharmacologyNorthwestern University ChicagoUSA

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