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Eicosanoid Formation and Regulation of Phospholipase A2

  • Eduardo G. Lapetina
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The oxygenated derivatives of arachidonic acid that are biologically active are defined as eicosanoids (Needleman et al., 1986). Among the eicosanoids are prostaglandins, including prostacyclin, thromboxanes, leukotrienes, and various hydroxy acids (Fig. 1). The eicosanoid precursor, arachidonic acid, is esterified in the 2-position of several phospholipids, and it must be hydrolyzed before the eicosanoids can be synthesized (Fig. 1). The liberated arachidonic acid can be enzymatically oxygenated by a membrane-bound cyclooxygenase, a microsomal cytochrome p450 or a cytosolic lipoxygenase with the formation of unstable intermediate products (Fig.1). These intermediate products include endoperoxides for prostaglandin production and epoxides and hydroperoxides for leukotriene and hydroxy acid formation (Fig 1). The type of arachidonate oxygenation is characteristic of the enzymes that each cell contains.

Keywords

Arachidonic Acid Pertussis Toxin Hydroxy Acid Eicosatrienoic Acid Eicosanoid Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cockcroft, S., and Gomperts, B. D., 1985, Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase, Nature, 314: 534.PubMedCrossRefGoogle Scholar
  2. Crouch, M. F., and Lapetina, E. G., 1988a, A role of Gi in control of thrombin receptor-phospholipase C in coupling human platelets, J. Biol. Chem., 263: 3363.PubMedGoogle Scholar
  3. Crouch, M. F., and Lapetina, E. G., 1988b, No direct correlation between Ca2+ mobilization and dissociation of Gi during platelet phospholipase A2 activation, Biochem. Biophys. Res. Comm., 153: 21.PubMedCrossRefGoogle Scholar
  4. Flower, R., 1981, Glucocorticoids, phospholipase A2 and inflammation, Trends Pharmacol. Sci., 2: 186.CrossRefGoogle Scholar
  5. Gryglewski, R. J., Bunting, S., Moncada, S., Flower, R. J., and Vane, J. R., 1976, Arterial walls are protected against deposition of platelet thrombi by a substance (prostaglandin X) which they make from prostaglandin endoperoxides, Prostaglandins, 12: 685.PubMedCrossRefGoogle Scholar
  6. Lapetina, E. G., 1982, Regulation of arachidonic acid production: Role of phospholipases C and A2, Trends Pharmacol Sci., 3: 115.CrossRefGoogle Scholar
  7. Lapetina, E. G., 1984, Phospholipases, in: “Annual Reports Medicinal Chemistry,” 19: 213.Google Scholar
  8. Lapetina, E. G., 1986, Inositide-dependent and -independent mechanisms in platelet activation, in: “Phosphoinositides and Receptor Mechanisms,” J. W. Putney, Jr., ed., p. 271, Alan R. Liss, Inc., New York.Google Scholar
  9. Lapetina, E. G., Lacal, J. C., Reep, B. R., and Molina y Vedia, L., 1989, A ras-related protein is phosphorylated and translocated by agonists that increase cyclic AMP levels in human platelets, Proc. Natl. Acad. Sci., 86: 3131.PubMedCrossRefGoogle Scholar
  10. Lewis, R. A., and Austen, K. F., 1984, The biologically active leukotrienes: Biosynthesis, metabolism, receptors, functions, and pharmacology, J. Clin. Invest., 73: 889.PubMedCrossRefGoogle Scholar
  11. Needleman, P., Tunk, J., Jakschik, B. A., Morrison, A. E., and Lefkowith, J. B., 1986, Arachidonic acid metabolism, Ann. Rev. Biochem., 55: 69.PubMedCrossRefGoogle Scholar
  12. Salmon, J. A., 1983, Measurement of eicosanoids by bioassay and radioimmunoassay, Br. Med. Bull., 39: 227.PubMedGoogle Scholar
  13. Samuelsson, B., 1981, Prostaglandins, thromboxanes, and leukotrienes: Formation and biological roles, in: “The Harvey Lectures,” Series 75, Academic Press, New York, NY, 1: 40.Google Scholar
  14. Samuelsson, B., 1983, Leukotrienes: Mediators of immediate hypersensitivity reactions and inflammation, Science, 220: 568.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Eduardo G. Lapetina
    • 1
  1. 1.Division Of Cell BiologyBurroughs Wellcome Co.Research Triangle ParkUSA

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