Liver Glutamate and Glutamine Concentration in Hepatocarcinogenesis Experimental: Effect of Glutathione
Among the metabolic alterations involved in thioacetamide (TAA) induced hepatocarcinogenesis, effects on ammonia metabolism have been demonstrated. This study was undertaken to investigate the response of liver glutamate and glutamine concentration after glutathione administration.
After 20 days of TAA administration, liver glutamate and glutamine concentration were 140% and 111% respectively of the control value. 2-oxoglutarate decreased to 25%. Glutamate dehydrogenase (GDH) as measured by glutamate formation and γ-glutamyl transferase (GGT) activities increased to 278% and 128%. Phosphate dependent glutaminase and glutamine synthetase decreased to 85% and 43%. Glutathione administration to the TAA treated animals showed effects on glutamate and glutamine concentration whose values decreased close to the control; 2-oxoglutarate increased. GDH activity decreased to values close to the control. No effect on glutaminase and glutamine synthetase activities was observed. The present results suggest that metabolic adaptation of liver in TAA animals treated for 20 days, lead to the synthesis of glutamate through GDH activity. Glutathione administration partially stops the enhanced content of glutamate and glutamine.
KeywordsGlutathione Glutamine Alanine Neuroblastoma Toxicology
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