Abstract
The double CPIA-test (cytochrome P-450 induction assay) as described by Lesca et al. (7) was used to discriminate between the forms of cytochrome P-450 [the phenobarbital (PB)-3-methylcholanthrene (3-MC)-type) induced by thiram, a dithiocarbamate fungicide. The test consisted of studies by the technique of Ames for the ability of the S-9 preparation obtained from livers of rats induced by thiram to cause metabolic activation of ethidium bromide (EtBr) (for 3-MC type) or cyclophosphamide (CPA) (for PB-type of cytochrome P-450). The mutagenicity of activated EtBr or CPA was checked with TA 98 and TA 100 strains of S. typhimurium respectively.
It was found that thiram at 100mg/kg of body weight, while only slightly increasing the 3-MC-type of cytochrome P-450 (400 his + revertants/ug of EtBr as compared to 2200 his + revertants/ug of EtBr for 80mg of 3-MC/kg of body weight) caused a noticeable induction of the PB-type (800 his + revertants/ 800 ug of CPA as compared to 1300 his + revertants/800 ug of CPA for 50mg of PB/kg of body weight). The direct measurements performed on livers of rats induced with thiram showed no overall changes in the level of cytochrome P-450 but a shift in absorption maximum to 449 nm.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lecointe, P., Bichet, N., Fraire, C. and Paoletti, C. (1981) Biochem. Pharmaco1.30, 601–609
Hales, B.F. and Jain, R. (1980) Biochem. Pharmacol., 29, 256–259
Hales, B.F. and Jain, R. (1980) Biochem. Pharmacol., 29, 2031–2037
Ames, B.N., McCann, J. and Yamasaki, E. (1975) Mutation Res 31, 347–364
Matsubora, T., Koike, M., Touchi, A. Tochino, Y. and Seguno, K. (1976) Anal. Biochem., 75, 596–603
Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951) J Biol Chem., 193, 265–270
Lesca, P. Fournier, A., Lecointe, P. and Cresteil, T. (1984) Mutation Res., 129, 299–310
Harada, N. and Omura, T. (1981) J. Biol. Chem. 89, 237–248
Le Prevost, E.T., Gretail, T., Columelli, S. and Leroux, J.P. (1983) Biochem Pharmacol
Dalvi, R.R., Robbins, T.J., Williams, M.K., Deoras, D.P., Donastorg, F. and Banks, C. (1984) J Environ. Sci Health, B19 (8 & 9), 703–712
Zemaitis, M.A., and Greene, F.E. (1979) Toxicol. and Applied Pharmacol., 48, 343–350
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Randen-Staron, I., Szumilo, M., Szymczyk, T. (1989). Effect of Thiram on Cytochrome P-450 Studied by the Cypia-Test. In: Garner, R.C., Hradec, J. (eds) Biochemistry of Chemical Carcinogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0539-2_17
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0539-2_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-7856-6
Online ISBN: 978-1-4613-0539-2
eBook Packages: Springer Book Archive