Analysis of the Ability of Spleen Cells from Aged Mice to Produce Allospecific Cytotoxic Cells

  • Susan R. S. Gottesman
  • J. M. Edington
Conference paper
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


The ability of aged rodents and humans to respond to foreign antigenic challenge decreases with age (Makinodan, 1977; Gottesman, 1987). This age-related deficiency is particularly severe in the T-cell system, as evidenced by the involution of the thymus, the decline in T- proliferative response to mitogens and specific antigens (Meredith and Walford, 1977; Miller and Stutman, 1981), the defeat in the ability to generate specific T-suppressor cells (Gottesman et al., 1984; Yin et al., 1988), and the inability of T cells to provide help for antibody production and cell-mediated immune responses (Miller and Stutman, 1981; Zharhary et al., 1984). Although many of these defects are contributed to by reduced lymphokine production by cells from aged donors (Miller and Stutman, 1981; Thoman and Weigle, 1982; Chang et al., 1982; Gilman et al., 1982), these activities are not all totally restored by addition of exogenous lymphokines (Gottesman et al., 1985). Cytotoxic T-lymphocyte (CTL) activity, a vital function for survival of the organism, shows an age-related decrease when assayed in bulk cultures (Gottesman et al., 1981). Limiting dilution analysis show a deficiency in a proportion of aged mice tested in CTL precursor frequency, under conditions in which helper cell function and interleukin-2 (IL-2) production are not limiting (Miller, 1984; Gorczynski and Chang, 1984; Zharhary et al., 1984).


Spleen Cell Aged Mouse Young Mouse Young Control Mixed Lymphocyte Culture 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Susan R. S. Gottesman
    • 1
  • J. M. Edington
    • 1
  1. 1.Department of PathologyNew York University Medical CenterNew YorkUSA

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