Analysis of the Ability of Spleen Cells from Aged Mice to Produce Allospecific Cytotoxic Cells
The ability of aged rodents and humans to respond to foreign antigenic challenge decreases with age (Makinodan, 1977; Gottesman, 1987). This age-related deficiency is particularly severe in the T-cell system, as evidenced by the involution of the thymus, the decline in T- proliferative response to mitogens and specific antigens (Meredith and Walford, 1977; Miller and Stutman, 1981), the defeat in the ability to generate specific T-suppressor cells (Gottesman et al., 1984; Yin et al., 1988), and the inability of T cells to provide help for antibody production and cell-mediated immune responses (Miller and Stutman, 1981; Zharhary et al., 1984). Although many of these defects are contributed to by reduced lymphokine production by cells from aged donors (Miller and Stutman, 1981; Thoman and Weigle, 1982; Chang et al., 1982; Gilman et al., 1982), these activities are not all totally restored by addition of exogenous lymphokines (Gottesman et al., 1985). Cytotoxic T-lymphocyte (CTL) activity, a vital function for survival of the organism, shows an age-related decrease when assayed in bulk cultures (Gottesman et al., 1981). Limiting dilution analysis show a deficiency in a proportion of aged mice tested in CTL precursor frequency, under conditions in which helper cell function and interleukin-2 (IL-2) production are not limiting (Miller, 1984; Gorczynski and Chang, 1984; Zharhary et al., 1984).
KeywordsSpleen Cell Aged Mouse Young Mouse Young Control Mixed Lymphocyte Culture
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- Gorczynski, R. M., and Chang, M.-P., 1984, Peripheral (somatic) expansion of the murine cytotoxic T lymphocyte repertoire. II. Comparison of diversity in recognition repertoire of alloreactive T cells in spleen and thymus of young or aged DBA/2J mice transplanted with bone marrow cells from young or aged donors, J. Immunol. 133: 2381–2389.PubMedGoogle Scholar
- Gottesman, S. R. S., 1987, T cell function in aging—An update, in: Review of Biological Research in Aging, Vol. 3 ( W. H. Adler, ed.), Liss, New York, pp. 95–110.Google Scholar
- Grimm, E. A., and Bonavida, B., 1977, Studies on the induction and expression of T cell mediated immunity. VI. Heterogeneity of lytic efficiency exhibited by isolated cytotoxic T lymphocytes prepared from highly enriched populations of effector-target conjugates, J. Immunol. 119: 1041–1047.PubMedGoogle Scholar
- Makinodan, T., 1977, Immunity and Aging, in: Handbook of the Biology of Aging ( C. E. Finch and L. Hayflick, eds.), Von Nostrand-Reinhold, New York, pp. 379–408.Google Scholar
- Yin, J. -Z., Gottesman, S. R. S., Bell, M. K., and Thorbecke, G. J., 1988, Resistance to low dose tolerance and enhanced antibody repsonses of aged as compared to young mice immunized with pneumococca; polysaccharides, Aging: Immunology and Infectious Disease 1: 131.Google Scholar