The Survival of a Chloramphenicol Resistance Plasmid in a Natural Bacillus Population
Bacillus species are important commercial sources of a variety of industrial enzymes including extracellular proteases and amylases. In addition, the insecticidal properties of Bacillus thuringiensis are widely employed for the bio-control of insect pests. Consequently, concerns have been voiced over the deliberate or accidental release of recombinant Bacillus strains into the environment, and the potential for gene survival and transfer. In this work a chloramphenicol resistance plasmid, pC194, has been released into a natural Bacillus population (in mushroom compost), that has had the levels of antibiotic resistance within the population determined: chloramphenicol; 5.6 × 103 cfu per g of compost.
KeywordsAgar Bacillus Amylase Chloramphenicol
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