Bioluminescent REC A Mutants of Rhizobium as Model Organisms in Risk Assessment Studies
A major concern about the deliberate release of genetically modified microorganisms (GEMMOs) is that the released organism exerts a potential disruptive effect on the biotic community of its target eco-system. Approaches which circumvent such a potential risk involve GEMMOs which are either genetically debilitated (Curtiss, 1988), or carry conditionally lethal genes (Molin et al., 1987; Bej et al., 1988; Contreras et al., 1991; Knudsen and Karlström, 1991). We propose the use of recA mutants of Rhizobium as hosts for use in genetic manipulations, since recA mutants show a reduced fitness (Selbitschka et al., 1991). Using newly developed recA integration vectors, bioluminescent recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae were constructed. We propose to consider such strains as ideal model organisms in risk assessment studies.
KeywordsSucrose Agar Citrate Streptomycin Tetracycline
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