Direct Detection of E.Agglomerans DNA Sequences in Soil

  • Sonja Selenska
  • Walter Klingmüller
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 63)


We present a non-radioactive detection of Tn5 and nif sequences of a GEMMO E.agglomerans 19-1-1 in EcoR I digested DNA recovered by a simple direct lysis procedure from inoculated soil samples, where the target bacteria no longer gave colonies on a selective agar medium.


Direct Detection Plasmid Transfer Target Bacterium Neomycin Phosphotransferase Gene Enterobacter Agglomerans 
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  1. Beck, E., Ludwig, G., Auerswald, E.A. and Schaller, H. (1982). Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from Tn5. Gene 19, 327–336.PubMedCrossRefGoogle Scholar
  2. Klingmüller, W. (1991) Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter. FEMS Microbiology Ecology 85, 107–116.CrossRefGoogle Scholar
  3. Klingmüller, W., Dally, A., Fentner, C. and Steinlein, M. (1990). Plasmid transfer between soil bacteria. In “Bacterial Genetics in Natural Environments,” (Fry, J.C. and Day, M.J. Eds) pp. 133–151. Chapman and Hall, LondonGoogle Scholar
  4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar
  5. Selenska, S. and Klingmüller, W. (1991). Direct detection of nif-gene sequences of Enterobacter agglomerans in soil. FEMS Microbiol. Letters 80, 243–246.CrossRefGoogle Scholar
  6. Selenska, S. and Klingmüller, W. (1991). DNA recovery and direct detection of Tn5 sequences from soil. Letters in Appl. Microbiol. 13, 21–24.Google Scholar
  7. Steffan, R. J., Coksoyr, J., Bej, A.K. and Atlas, R.M. (1988). Recovery of DNA from soils and sediments. Appl. Environ. Microbiol. 54, 2908–2915.PubMedGoogle Scholar
  8. Tsai, Y. and Olson, B.H. (1991). Rapid method for direct extraction of DNA from soil and sediments. Appl. Environ. Microbiol. 57, 1070–1074.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1992

Authors and Affiliations

  • Sonja Selenska
    • 1
  • Walter Klingmüller
    • 1
  1. 1.Department of GeneticsUniversity of BayreuthBayreuthGermany

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