Direct Detection of E.Agglomerans DNA Sequences in Soil

  • Sonja Selenska
  • Walter Klingmüller
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 63)

Abstract

We present a non-radioactive detection of Tn5 and nif sequences of a GEMMO E.agglomerans 19-1-1 in EcoR I digested DNA recovered by a simple direct lysis procedure from inoculated soil samples, where the target bacteria no longer gave colonies on a selective agar medium.

Keywords

Agar Codon Agarose Electrophoresis dNTP 

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References

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Copyright information

© Plenum Press, New York 1992

Authors and Affiliations

  • Sonja Selenska
    • 1
  • Walter Klingmüller
    • 1
  1. 1.Department of GeneticsUniversity of BayreuthBayreuthGermany

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