Direct Detection of E.Agglomerans DNA Sequences in Soil
We present a non-radioactive detection of Tn5 and nif sequences of a GEMMO E.agglomerans 19-1-1 in EcoR I digested DNA recovered by a simple direct lysis procedure from inoculated soil samples, where the target bacteria no longer gave colonies on a selective agar medium.
KeywordsAgar Codon Agarose Electrophoresis dNTP
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- Klingmüller, W., Dally, A., Fentner, C. and Steinlein, M. (1990). Plasmid transfer between soil bacteria. In “Bacterial Genetics in Natural Environments,” (Fry, J.C. and Day, M.J. Eds) pp. 133–151. Chapman and Hall, LondonGoogle Scholar
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar
- Selenska, S. and Klingmüller, W. (1991). DNA recovery and direct detection of Tn5 sequences from soil. Letters in Appl. Microbiol. 13, 21–24.Google Scholar