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Molecular approaches to improving the nutritional and functional properties of oilseeds, grains and legumes

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Food and Feed from Legumes and Oilseeds

Abstract

The purpose of this review is to discuss the rationale for improving the nutritional and functional properties of plant seeds through genetic engineering, and to point out recent developments in molecular biology of seed development and plant biotechnology that are relevant to this goal. Since this is not meant to be a comprehensive review, the reader is referred to more complete reviews on biochemistry and molecular biology of seed proteins written in the last ten years (Shewry and Miflin, 1984; Higgins, 1984; Croy and Gatehouse, 1985; Casey et al., 1986; Gatehouse et al., 1986; Goldberg, 1986; Goldberg et al., 1989; Shewry et al., 1989).

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References

  • Abbott, J.C. (1966) Protein supplies and prospects: the problem, in World Protein Resources, (ed. R.F. Gould), ACS Washington, DC, pp. 1–15.

    Chapter  Google Scholar 

  • Allen, R.D., Bernier, F., Lessard, P.A. and Beachy, R.N. (1989) Nuclear factors that interact with a soybean β-conglycinin enhancer. Plant Cell, 1, 623–31.

    Article  CAS  Google Scholar 

  • Altenbach, S.B., Pearson, K.W., Leung, F.W. and Sun, S.S.M. (1987) Cloning and sequence analysis of a cDNA encoding a Brazil nut protein exceptionally rich in methionine. Plant Mol Biol, 8, 239–50.

    Article  CAS  Google Scholar 

  • Altenbach, S.B., Pearson, K.W., Meeker, G. et al. (1989) Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants. Plant Mol Biol, 13, 513–22.

    Article  CAS  Google Scholar 

  • Argos, P., Pedersen, K., Marks, M.D. and Larkins, B.A. (1982) A structural model for maize zein proteins, J. Biol. Chem., 257, 9984–90.

    CAS  Google Scholar 

  • Barker, S.J., Harada, J.J. and Goldberg, R.B. (1988) Cellular localization of soybean storage protein mRNA in transformed tobacco seeds. Proc. Nat Acad. Sci. USA, 85, 458–62.

    Article  CAS  Google Scholar 

  • Battey, J.F. and Ohlrogge, J.B. (1989) A comparison of the metabolic fate of fatty acids of different chain lengths in developing oilseeds. Plant Physiol, 90, 835–40.

    Article  CAS  Google Scholar 

  • Baumlein, H., Wobus, U., Pustell, J. and Kafatos, F.C. (1986) The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element. Nucl. Acids Res., 14, 2707–20.

    Article  CAS  Google Scholar 

  • Beach, L.R., Spencer, D., Randall, P.J. and Higgins, T.J.V. (1985) Transcriptional and posttranscriptional regulation of storage protein gene expression in sulfur deficient pea seeds. Nucl Acids Res., 13, 999–1013.

    Article  CAS  Google Scholar 

  • Benfey, P.N. and Chua, N.H. (1990) The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants. Science, 250, 959–66.

    Article  CAS  Google Scholar 

  • Blundell, T. and Sternberg, M.J. (1985) Computer aided design in protein engineering. Trends Biotechnol, 3, 229–31.

    Article  Google Scholar 

  • Bodwell, CE. (1987) Nutritional implications of cereals, legumes and their products, in Cereals and Legumes in the Food Supply, (eds J. Dupont and E.M. Osman), Iowa State University Press, Ames, IÁ, pp. 259–75.

    Google Scholar 

  • Boston, R.S., Kodrycki, R. and Larkins, B.A. (1986) Transcriptional and posttranscriptional regulation of maize zein genes, in Molecular Biology of Seed Storage Proteins and Lectins, (eds L.M. Shannon and M.J. Chrispeels), American Society of Plant Physiologists.

    Google Scholar 

  • Bray, E.A., Naito, S., Pan, N.S. et al. (1987) Expression of the β-subunit of β-conglycinin in seeds of transgenic plants. Planta, 172, 364–70.

    Article  CAS  Google Scholar 

  • Bressani, R. and Elias, L.G. (1968) Processed vegetable protein mixtures for human consumption in developing countries. Adv. Food Res., 16, 1–103.

    Article  CAS  Google Scholar 

  • Bressani, R., Elias, L.G. and Gomez-Brenes, R.A. (1969) Protein quality of opaque-2 corn evaluation in rats. J. Nutr., 97, 173–80.

    CAS  Google Scholar 

  • Burr, F.A. and Burr, B. (1982) Three mutations in Zea mays affecting zein accumulation: a comparison of zein polypeptides, in vitro synthesis and processing, mRNA levels and genomic organization, J. Cell Biol, 94, 201–6.

    Article  CAS  Google Scholar 

  • Burr, B., Burr, F.A., St John, T.P. et al. (1982) Zein storage gene family of maize: an assessment of heterogeneity with cloned mRNA sequences. J. Mol. Biol, 154, 33–49.

    Article  CAS  Google Scholar 

  • Bustos, M.M., Guiltinan, M.J., Jordano, J. et al. (1989) Regulation of B-lucuronidase expression in transgenic tobacco plants by an A/T-rich, cis-acting sequence found upstream of a French bean B–phaseolin gene. Plant Cell, 1, 839–53.

    Article  CAS  Google Scholar 

  • Butz, E.L. (1973) World protein markets — a supplier’s view. Plenary Session of World Protein Markets, World Soybean Conference Nov. 11–14, Munich, Germany.

    Google Scholar 

  • Cagampang, G.B., Cruz, L.J. and Juliano, B.O. (1971) Free amino acids in the bleeding sap and developing grain of the rice plant. Cereal Chem., 48, 533–9.

    CAS  Google Scholar 

  • Calloway, D.H. and Murphy, E.L. (1968) The use of expired air to measure intestinal gas formation. Ann. NY Acad. Sci., 150, 82–5.

    Article  CAS  Google Scholar 

  • Casey, R., Downey, C. and Ellis, N. (1986) Legume storage proteins and their genes. Oxford Surv. Plant Mol. Cell Biol. 3, 1–95.

    CAS  Google Scholar 

  • Castillo, E.M., de Lumen, B.O., Reyes, P.S. and de Lumen, H.Z. (1990) Raffinose synthase and galactinol synthase in developing seeds and leaves of legumes. J. Agric. Food Chem., 38, 351–5.

    Article  CAS  Google Scholar 

  • Chappell, J. and Chrispeels, M.J. (1986) Transcriptional and posttranscriptional control of phaseolin and phytchemagglutinin gene expression in developing cotyledons of Phaseolus vulgaris. Plant Physiol, 81, 50–4.

    Article  CAS  Google Scholar 

  • Chen, Z.L., Schuller, M.A. and Beachy, R.N. (1986) Functional analysis of regulatory elements in a plant embryo—specific gene. Proc. Natl Acad. Sci. USA, 83, 8560–4.

    Article  CAS  Google Scholar 

  • Chen, Z.L., Pan, N.S. and Beachy, R.N. (1988) A DNA sequence element that confers seed–specific enhancement to a constitutive promoter. EMBO J., 7, 297–302.

    CAS  Google Scholar 

  • Chourey, P.S. (1981) Genetic control of sucrose synthetase in maize endosperm. Mol. Gen. Genet., 184, 372–6.

    Article  CAS  Google Scholar 

  • Chourey, P.S. (1982) Starch mutants and their protein products, in Maize for Biological Research (ed. W.F. Sheridan), Plant Molecular Biology Association Charlottesville, VA, pp. 129–33.

    Google Scholar 

  • Chourey, P.S. and Nelson, O.E. (1976) The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem. Genet., 14, 1041–55.

    Article  CAS  Google Scholar 

  • Chourey, P.S., Latham, M.D. and Still, P.E. (1986) Expression of two sucrose synthetase genes in endosperm and seedlings of maize: evidence of tissue specific polymerization of protomers. Mol Gen. Genet., 203, 251–5.

    Article  CAS  Google Scholar 

  • Christou, P., McCabe, D.E. and Swain, W.F. (1988) Stable transformation of soybean callus by DNA–coated gold particles. Plant Physiol, 87, 671–4.

    Article  CAS  Google Scholar 

  • Christou, P., Swain, W.F., Yang, N.S. and McCabe, D.E. (1989) Inheritance and expression of foreign genes in transgenic soybean plants. Proc. Natl Acad. Sci USA, 86, 7500–4.

    Article  CAS  Google Scholar 

  • Christou, P., McCabe, D.E., Martinell, B.J. and Swain, W.F. (1990) Soybean genetic engineering — commercial production of transgenic plants. TIBTECH, 8, 145–51.

    CAS  Google Scholar 

  • CIMMYT (Centro Internacional de Mejoramiento de Maiz y Trigo). (1985) Boosting protein quality in maize. CIMMYT Research Highlights 1984, CIMMYT Mexico.

    Google Scholar 

  • Cleveland, T.E., Thornburg, R.W. and Ryan, C.A. (1987) Molecular characterization of a wound–inducible inhibitor I gene from potato and processing of its mRNA and protein. Plant Mol. Biol, 8, 119–207.

    Article  Google Scholar 

  • Cocking, E.C. and Davey, M.R. (1987) Gene transfer in cereals. Science, 236,1259–62.

    Article  CAS  Google Scholar 

  • Creech, R.G. (1968) Carbohydrate synthesis in maize. Adv. Agron., 20, 275–322.

    Article  CAS  Google Scholar 

  • Croy, R.R.D. and Gatehouse, J.A. (1985) Genetic engineering of seed proteins: current and potential applications, in Plant Genetic Engineering, (ed. J.H. Dodds), Cambridge University Press, Cambridge, pp. 143–268.

    Google Scholar 

  • Croy, R.R.D., Lycett, G.W., Gatehouse, J.A. et al. (1982) Cloning, characterization and sequence analysis of cDNAs encoding pea storage protein precursors-legumin. Nature, 295, 76–9.

    Article  CAS  Google Scholar 

  • de Clerq, A., Vandewiele, M., Van Damme, J. et al. (1990) Stable accumulation of modified 2S albumin seed storage proteins with higher methionine contents in transgenic plants. Plant Physiol, 94, 970–9.

    Article  Google Scholar 

  • de la Pena, A., Lorz, H. and Schell, J. (1987) Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature, 325, 274–5.

    Article  Google Scholar 

  • de Lumen, B.O. and Chan, J. (1986) Antinutritional and biochemical properties of winged bean trypsin inhibitors, in Nutritional and Toxicological Significance of Enzyme Inhibitors in Foods, (ed. M. Friedman), pp. 413–27.

    Google Scholar 

  • de Lumen, B.O. and Kho, C.J. (1987) Identification of methionine containing proteins and quantitation of their methionine contents, J. Agric Food Chem., 35, 688–91.

    Article  Google Scholar 

  • Dey, P.M. (1985) D-Galactoside containing oligosaccharides, in Biochemistry of Storage Carbohydrates in Green Plants, (eds P.M. Dey, and R.A. Dixon) Academic Press, New York, pp. 53–129.

    Google Scholar 

  • di Fonzo, N., Gentinetta, E., Salamini, F. and Soave, C. (1980) Action of the opaque-7 mutation on the accumulation of storage products in maize endosperm, Plant Sci., 14, 345–54.

    Google Scholar 

  • Doll, H. (1984) Nutritional aspects of cereal proteins and approaches to overcome their deficiencies. Phil Trans. R. Soc. Lond., B304, 373–80.

    Google Scholar 

  • Doll, H., Kole, B. and Eggum, B.O. (1974) Induced high–lysine mutants in barley, Radiat. Bot., 14, 73–80.

    Article  CAS  Google Scholar 

  • Domoney, C. and Casey, R. (1985) Measurement of gene number for seed storage proteins in Pisum. Nucl. Acids Res., 13, 687–99.

    Article  CAS  Google Scholar 

  • Duffus, CM. and Slaughter, J.C. (1980) Seeds and Their Uses. Wiley, New York.

    Google Scholar 

  • Dynan, W.S. and Tjian, R. (1985) Control of eukaryotic mRNA synthesis by sequence specific DNA binding proteins. Nature, 316, 774–8.

    Article  CAS  Google Scholar 

  • Eggum, B.O. and Beames, R.M. (1983) The nutritive value of seed proteins, in Seed Proteins: Biochemistry, Genetics, Nutritive Value, (eds W. Gottschalk and H.P. Muller), Martinus Nijhoff, The Hague, pp. 499–531.

    Google Scholar 

  • Elhussein, S.A., Miernyk, J.A. and Ohlrogge, J.B. (1987) A preliminary characterization of plant holo–acyl carrier protein synthase, in The Metabolism, Structure, and Function of Plant Lipids, (eds P.K. Stumpf, J.B. Mudd and W.D. Ness), Plenum Press, New York, pp. 709–13.

    Google Scholar 

  • Elliston, K.O, Imran, S. and Messing, J. (1988) Cloned genes of storage proteins. Plant Mol Biol. Rep., 6, 22–6.

    Article  CAS  Google Scholar 

  • Evans, I.M., Gatehouse, J.A., Croy, R.R.D. and Boulter, D. (1984) Regulation of transcription of storage protein mRNA in nuclei isolated from developing pea cotyledons. Planta, 160, 559–68.

    Article  CAS  Google Scholar 

  • FAO (1957) Protein Requirements FAO Nutr. Studies No.16, FAO, Rome.

    Google Scholar 

  • FAO (1980) Food Balance Sheets, 1975–1977. Food and Agriculture Organization of the UN.

    Google Scholar 

  • FAO/WHO (1973) Energy requirements and protein requirements. WHO Tech Rep Ser No. 522.

    Google Scholar 

  • FAO/WHO/UNU (1985) Energy and Protein Requirements. WHO Technical Report Series 724. WHO Geneva, Switzerland.

    Google Scholar 

  • Fraley, R.T., Rogers, S.G. and Horsch, R.B. (1986) Genetic transformation in higher plants. CRC Crit Rev. Plant Sci., 4, 1–46.

    Article  CAS  Google Scholar 

  • Fromm, M, Taylor, L.P. and Talbot, V. (1986) Stable transformation of maize after gene transfer by electroporation. Nature, 319, 791–3.

    Article  CAS  Google Scholar 

  • Gasser, C.S. and Fraley, R.T. (1989) Genetically engineering plants for crop improvement. Science, 236, 1293–9.

    Article  Google Scholar 

  • Gatehouse, J.A., Evans, I.M., Croy, R.R.D. and Boulter, D. (1986) Differential expression of genes during seed development. Phil. Trans. R. Soc. Lond., B314, 367–84.

    Google Scholar 

  • George, A. and de Lumen, B.O. (1991) A novel methionine rich protein in soybean seed: identification, amino acid composition and N-terminus sequence. J. Agric. Food Chem., 39, 224–70.

    Article  CAS  Google Scholar 

  • Gobel, E. and Lorz, H. (1988) Genetic manipulation of cereals. Oxford Surv. Plant Mol. Cell Biol, 5, 1–22.

    Google Scholar 

  • Goldberg, R.B. (1988) Plants: novel developmental processes. Science, 240,1460–6.

    Article  CAS  Google Scholar 

  • Goldberg, R.B. (1986) Regulation of plant gene expression. Phil. Trans. R. Soc. Lond., B314, 343–53.

    Google Scholar 

  • Goldberg, R.B., Hoschek, G., Tarn, S.H. et al. (1981) Abundance, diversity and regulation of mRNA sequence sets in soybean embryogenesis. Dev. Biol, 83, 201–17.

    Article  CAS  Google Scholar 

  • Goldberg, R.B., Barker, S. and Perez–Grau, L. (1989) Regulation of gene expression during plant embryogenesis. Cell, 56, 149–60.

    Article  CAS  Google Scholar 

  • Graham, J., Hall, G., Pearce, G. and Ryan, C.A. (1986) Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants. Planta, 169, 399–405.

    Article  CAS  Google Scholar 

  • Guerche, P., De Almeida, E.R.P., Schwartzstein, M.A. et al. (1990) Expression of the 2S albumin from Berthellotia excelsa in Brassica napus. Mol. Gen. Genet., 221, 306–14.

    Article  CAS  Google Scholar 

  • Guerra, D.J., Ohlrogge, J.B. and Frentzen, M. (1987) A possible differential role for plant acyl carrier protein isoforms in higher plants, in The Metabolism, Structure and Function of Plant Lipids (eds P.K. Stumpf, J.B. Mudd and W.D. Ness), Plenum Press, New York, pp. 705–8.

    Google Scholar 

  • Gupta, M., Chourey, P.S., Burr, P. and Still, P. (1988) cDNAs of two non-allelic sucrose synthase genes in maize: cloning expression, characterization and molecular mapping of the sucrose synthase–2 gene. Plant Mol. Biol, 10, 215–24.

    Article  CAS  Google Scholar 

  • Gupta, Y.P. (1983) Nutritive value of food legumes, in Chemistry and Biochemistry of Legumes, (ed. S.K. Arora), Oxford and IBH Publishing Co., New Delhi, India.

    Google Scholar 

  • Hagen, G. and Rubinstein, I. (1981) Complex organization of zein genes in maize. Gene, 13, 239–49.

    Article  CAS  Google Scholar 

  • Hammond, R.W., Foard, D.E. and Larkins, B.A. (1984) Molecular cloning and analysis of a gene coding for the Bowman-Birk protease inhibitor in soybean. J. Biol. Chem., 259, 9883–90.

    CAS  Google Scholar 

  • Harada, J.J., Barker, S.J. and Goldberg, R.B. (1989) Soybean β–conglycinin genes are clustered in several DNA regions and are regulated by transcriptional and posttranscriptional processes. Plant Cell, 1, 415–25.

    Article  CAS  Google Scholar 

  • Hartings, H., Maddaloni, M., Lazzaroni, N. et al. (1989) The 02 gene which regulates zein protein deposition in maize endosperm encodes a protein with structural homologies to transcriptional activators. EMBO J., 8, 2795–801.

    CAS  Google Scholar 

  • Higgins, T.J.V. (1984) Synthesis and regulation of major seed proteins in seeds Annu. Rev. Plant Physiol, 35, 191–221.

    Article  CAS  Google Scholar 

  • Higuchi, M., Suga, M. and Iwai, K. (1983) Participation of lectin in biological effects of raw winged bean seeds on rats. Agric. Biol. Chem., 47, 1879–86.

    Article  CAS  Google Scholar 

  • Hildebrand, D.F. and Hymowitz, T. (1981) Two soybean genotypes lacking lipoxygenase, J. Am. Oil Chem. Soc., 58, 583–6.

    Article  CAS  Google Scholar 

  • Hildebrand, D.F., Altschule, M. et al. (1987) Physiological and transformational analyses of lipoxygenase, in The Metabolism, Structure and Function of Plant Lipids, (eds P.K. Stumpf, J.B. Mudd and W.D. Ness), Plenum Press, New York, pp. 715–17.

    Google Scholar 

  • Hilder, V.A., Gatehouse, A.M.R., Sheerman, S.E. et al. (1987) A novel mechanism of insect resistance engineered into tobacco. Nature, 330, 160–3.

    Article  CAS  Google Scholar 

  • Hofman, L.M., Donaldson, D.D. and Herman, E.M. (1988) A modified storage protein is synthesized, processed and degraded in the seeds of transgenic plants. Plant Mol. Biol, 11, 717–29.

    Article  Google Scholar 

  • Hol, W.G.J. (1987) Applying knowledge of protein structure and function. Trends Biotechnol, 5, 137–43.

    Article  CAS  Google Scholar 

  • Horsch, R.B., Fry, J.E. et al. (1985) A simple and general method for transferring genes into plants. Science, 227, 1229–31.

    Article  CAS  Google Scholar 

  • Hugly, S., Kunst, L., Browse, J. and Somerville, C.R. (1989) Enhanced thermal tolerance of photosynthesis and altered choloplast ultrastructure in a mutant of Arabidopsis deficient in lipid desaturation. Plant Physiol, 90, 1134–42.

    Article  CAS  Google Scholar 

  • Hymowitz, T. (1987), increasing yields, food and nutritional quality through breeding: grain legumes, in Cereals and Legumes in the Food Supply, (eds J. Dupont and E.M. Osman) Iowa State Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA, (1990) Economic Implications of Modified Soybean Traits. Special Report 92, pp. 15–24. 88pp.

    Google Scholar 

  • Jaynes, J., Yang, M., Espinoza, M. and Dodds, J. (1986) Plant protein improvement by genetic engineering: use of synthetic genes. Trends Biotechnol, 4, 314–20.

    Article  CAS  Google Scholar 

  • Jofuku, K.D. and Goldberg, R.B. (1989) Kunitz trypsin inhibitor genes are differentially expressed during the soybean life cycle and in transformed tobacco plants. Plant Cell, 1, 1079–93.

    Article  CAS  Google Scholar 

  • Jofuku, K.D., Okamuro, J.K. and Goldberg, R.B. (1987) Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region. Nature, 328, 734r–7.

    Article  Google Scholar 

  • Jones, R.A., (1978) Effects of floury—2 locus on accumulation and RNA metabolism during endosperm development. Biochem. Genet., 16, 27–38.

    Article  CAS  Google Scholar 

  • Jones, R.A., Larkins, B.A. and Tsai, C.Y. (1977) Storage protein synthesis in maize II. Reduced synthesis of a major zein component by the opaque—2 mutant of maize. Plant Physiol, 59, 525–9.

    Article  CAS  Google Scholar 

  • Jordano, J., Almoguera, C. and Thomas, T.L. (1989) A sunflower helianthin gene upstream sequence ensemble contains an enhancer and sites of nuclear protein interaction. Plant Cell, 1, 855–66.

    Article  CAS  Google Scholar 

  • Joudrier, P.E., Foard, D.E., Floener, L.A. and Larkings, B.A. (1987) Isolation and sequence of cDNA encoding the soybean protease inhibitors PI IV and C-II. Plant Mol. Biol, 10, 35–42.

    Article  CAS  Google Scholar 

  • Juliano, B.O. (ed.) (1985) Rice: Chemistry and Technology. American Association ofCereal Chemists, St Paul, MN, pp. 1–16.

    Google Scholar 

  • Kadonaga, J.T, Jones, K.A. and Tjian, R. (1986) Promoter–specific activation of RNA polymerase II transcription by Spi. Trends Biochem., 11, 20–3.

    Article  CAS  Google Scholar 

  • Kandler, O. and Hopf, H. (1980) Metabolism and function of oligosaccharides, in Biochemistry of Plants, Vol. 3 Carbohydrates, (ed. J. Preiss), Academic Press, New York, pp. 231–70.

    Google Scholar 

  • Kay, R., Chan, A., Daly, M. and McPherson, J. (1987) Duplication of CaMV 35S. promoter sequences creates a strong enhancer for plant genes. Science, 236, 1299–302.

    Article  CAS  Google Scholar 

  • Kho, C.J. and de Lumen, B.O. (1988) Identification and isolation of methionine-cysteine rich proteins in soybean seed. Plant Foods Hum. Nutr., 38, 287–96.

    Article  CAS  Google Scholar 

  • Kim, C.S., Kamiya, S., Kanamori, J. et al. (1990a) High level expression, purification and functional properties of soybean proglycinin from Escherichia coli. Agric. Biol. Chem. 54, 1543–50.

    Article  CAS  Google Scholar 

  • Kim, C.S., Kamiya, S., Sato, T. et al. (1990b) Improvement of nutritional and functional properties of soybean glycinin through protein engineering. Protein Eng., 3, 725–31.

    Article  CAS  Google Scholar 

  • Kinsella, J. (1979) Functional properties of soy proteins. J. Am. Oil Chem. Soc, 56, 242–58.

    Article  CAS  Google Scholar 

  • Kitamura, K. (1984) Biochemical characterization of lipoxygenase lacking mutants, L-l Less, L-2 Less, L-3 Less soybeans. Agrie Biol. Chem., 48, 2339–46.

    Article  CAS  Google Scholar 

  • Kitamura, K., Davies, CS., Kaizuma, K. and Nielsen, N.C. (1983) Genetic analysis of a null allele for lipoxygenase-3 in soybean seeds. Crop Sci., 23, 924–7.

    Article  CAS  Google Scholar 

  • Klee, H., Horsch, R. and Rogers, S. (1987) Agrobacterium-mediated plant transformation and its further application to plant biology. Annu. Rev. Plant Physiol., 38, 467–86.

    Article  CAS  Google Scholar 

  • Kodrzycki, R., Boston, R.S. and Larkins, B.A. (1989) The opaque-2 mutation of maize differentially reduces zein gene transcription. Plant Cell, 1, 105–14.

    Article  CAS  Google Scholar 

  • Kreis, M, Shewry, P.R., Forde, B.G. et al. (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxford Surv. Plant Mol. Cell Biol., 2, 253–317.

    CAS  Google Scholar 

  • Krishnan, H.B., Reeves, CD. and Okita, T.W. (1986) ADP-Pyrophosphorylase is encoded by different mRNA transcripts in leaf and endosperm of cereals. Plant Physiol., 81, 642–5.

    Article  CAS  Google Scholar 

  • Kuhlemeir, C, Green, P.J., Chua, N.H. (1987) Regulation of gene expression in higher plants. Annu. Rev. Plant Physiol., 38, 221–466.

    Article  Google Scholar 

  • Kunst, L. and Somerville, CR. (1989) A mutant of Arabidopsis deficient in desaturation of palmitic acid in leaf lipids. Plant Physiol., 90, 943–7.

    Article  CAS  Google Scholar 

  • Lawrence, MC, Susuki, E., Varghese, J.N. et al. (1990) The three-dimensional structure of the seed storage protein phaseolin at 3 A resolution. EMBO J., 9,9–15.

    CAS  Google Scholar 

  • Lee, J.L., Brown, W.E., Pearce, G. et al. (1986) Molecular characterization and phylogenic studies of a wound-inducible proteinase inhibitor I gene in Lycopersicon species. Proc. Natl Acad. Sci. USA, 83, 7277–81.

    Article  CAS  Google Scholar 

  • Lee, P.D. and Su, J.C. (1982) Sucrose-starch transforming system in rice grains - a tracer feeding study. Proc. Natl Sci. Counc. Rep. China, B6, 189–96.

    Google Scholar 

  • Liener, I.E. and Kakade, M.L. (1969) Protease inhibitors, in Toxic Constituents of Plant Foodstuffs, (ed. I.E. Liener), Academic Press, New York.

    Google Scholar 

  • Lilley, G.G., Caldwell, J.B. and Kortt, A.A. (1989) Isolation and primary structure for a novel, methionine-rich protein from sunflowerseeds (Helianthus annuus L.) Proceedings of the World Congress on Vegetable Protein Utilization in Human Foods and Animal Feedstuffs, (ed. T.H. Applewhite), pp. 497–502.

    Google Scholar 

  • Lin, Y.L. (1986) Genetic engineering and process development for production of food processing enzymes and additives. Food Technol., 40, 104–6.

    CAS  Google Scholar 

  • Luo, Z. and Wu, R. (1988) A simple method for the transformation of rice via the pollen tube pathway. Plant Mol. Biol. Rep. 6, 165–74.

    Article  CAS  Google Scholar 

  • Maier, U.G., Brown, J.W.S. and Faix, G. (1987) Binding of a nuclear factor to a consensus sequence in the 5 ’ -flanking regions of zein genes from maize. EMBO J., 6, 17–22.

    CAS  Google Scholar 

  • Manners, D.J. (1988) Starch, in Biochemistry of Storage Carbohydrates in Plants, (eds P.M. Dey and R.A. Dixon), Academic Press, London, pp. 149–203.

    Google Scholar 

  • Marks, M.D., Lindell, J.S. and Larkins, B.A. (1985) Quantitative analysis of the accumulation of zein mRNA during maize endosperm development, J. Biol. Chem., 260, 16445–50.

    CAS  Google Scholar 

  • McCabe, D.E., Swain, W.F. et al. (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology, 6, 923–6.

    Article  Google Scholar 

  • McCarty, D., Shaw, J. and Hannah, L. (1986) The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc. Natl Acad. Sci. USA, 83, 9099–103.

    Article  CAS  Google Scholar 

  • Mertz, E.T., Bates, L.S. and Nelson, O.E. (1964) Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science, 145, 279–80.

    Article  CAS  Google Scholar 

  • Mertz, E.T., Veron, O.A., Bates, L.S. and Nelson, O.E. (1965) Growth of rats fed on opaque-2 maize. Science, 148, 1741–2.

    Article  CAS  Google Scholar 

  • Motto, M., Maddaloni, M., Onziani, G, et al. (1988) Molecular cloning of the o2–m5 allele of Zea mays using transposon marking. Mol. Gen. Genet., 212, 488–94.

    Article  CAS  Google Scholar 

  • Muntz, K. (1989) Intracellular protein sorting and the formation of protein reserves in storage tissue cells of plant seeds. Biochem. Physiol Pflanzen., 185, 315–35.

    Google Scholar 

  • Nakai, S. and Powrie, W.D. (1981) Modifications of proteins for functional and nutritional improvements, in Cereals — A Renewable Resource, Theory and Practice, (eds. Y. Pomeranz and L. Munck), American Association of Cereal Chemists, St Paul, MN, pp. 217–42.

    Google Scholar 

  • National Research Council (1988) Quality Protein Maize. National Academy Press, Washington, DC, p. 6.

    Google Scholar 

  • Nelson, O.E. and Rines, H.W. (1962) The enzymatic deficiency in waxy mutants of maize. Biochem. Biophys. Res. Commun., 9, 297–300.

    Article  CAS  Google Scholar 

  • Neuhaus, G, Spangenberg, G. et al. (1987) Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids. Theor. Appi Genet., 74, 30–6.

    Google Scholar 

  • Nielsen, N.C., Dickinson, CD., Cho, T.J. et al. (1989) Characterization of the glycinin gene family in soybean. Plant Cell., 1, 313–28.

    Article  CAS  Google Scholar 

  • Odigboh, E.U. (1983) Cassava: production, processing and utilization, in Handbook of Tropical Foods. (ed. H.T. Chan), Marcel Dekker, New York, pp. 145–202.

    Google Scholar 

  • Ohlrogge, J.B., Beremand, P.D., Hannapel, D.J. et al. (1987) Acyl carrier protein as a probe of the molecular biology of plant fatty acid synthesis, in The Metabolism, Structure, and Function of Plant Lipids, (eds. P.K. Stumpf, J.B. Mudd and W.D. Ness), Plenum Press, New York, pp. 689–95.

    Google Scholar 

  • Okamuro, J.K., Jofuko, K.D. and Goldberg, R.B. (1986) Soybean lectin gene and flanking non-seed protein genes are developmentally regulated in transformed tobacco plants. Proc. Natl Acad. Sci. USA, 83, 8240–4.

    Article  CAS  Google Scholar 

  • Okuno, K. (1976) A low-amylose mutant of rice. Div. Genet. Natl Inst. Agrie. Sci. Japan. Ann. Rep., 1, 28–9.

    Google Scholar 

  • Ovcharov, K.G. and Koshelev, Y.P. (1974) Sugar content in com seeds with different viability. Fiziol. Rast., 21, 969–4.

    CAS  Google Scholar 

  • Payne, P.I., Corfield, K.G, Holt, L.M. and Blackman, J.A. (1981) Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and breadmaking quality in progenies of six crosses of bread wheat. J. Sci. Food Agrie., 32, 51–60.

    Article  CAS  Google Scholar 

  • Pedersen, K., Deverux, J, Wilson, D. et al. (1982) Cloning and sequence analysis reveal structural variation among related zein genes in maize. Cell, 29,1015–26.

    Article  CAS  Google Scholar 

  • Perez, CM., Cagampang, G.B., Esmana, B.V. et al. (1973) Protein metabolism in leaves and developing grains of rices differing in grain protein content. Plant Physiol, 51, 537–42.

    Article  CAS  Google Scholar 

  • Perez-Grau, L. and Goldberg, R.B. (1989) Soybean seed protein genes are regulated spatially during embryogenesis. Plant Cell, 1, 1095–109.

    Article  CAS  Google Scholar 

  • Potrykus, I. (1990) Gene transfer to plants: assessment and perspectives. Physiol. Plant., 79, 125–34.

    Article  CAS  Google Scholar 

  • Preiss, J. (1982) Regulation of the biosynthesis and degradation of starch. Annu. Rev. Plant Physiol., 33, 431–54.

    Article  CAS  Google Scholar 

  • Rackis, J. (1981) Flatulence caused by soya and its control through processing. J. Am. Oil Chem. Soc., 58, 503–9.

    Article  CAS  Google Scholar 

  • Raven, P.H., Evert, R.F. and Eichhorn, S.E. (1987) Biology of Plants, 4th edn, Worth Publishers, New York, pp. 373–83.

    Google Scholar 

  • Riggs, CD., Voelker, T.A. and Chrispeels, M.J. (1989) Cotyledon nuclear proteins bind to DNA fragments harboring regulatory elements of phytohemagglu-tinin genes. Plant Cell, 1, 609–21.

    Article  CAS  Google Scholar 

  • Roxas, B.V., Intengan, C. Ll. and Juliano, B.O. (1979) Protein quality of high protein and low protein milled rices in preschool children. J. Nutr., 109,832–9.

    CAS  Google Scholar 

  • Ryan, C.A. (1981) Proteinase inhibitors, in The Biochemistry of Plants. A Comprehensive Treatise, (eds P.K. Stumpf and E.E. Conn), Vol. 6, Academic Press, New York, pp. 351–71.

    Google Scholar 

  • Ryan, CA. 1981 Proteinase inhibitor gene families: strategies for transformation to improve plant defences against herbivores. Bioessays, 10, 20–4.

    Article  Google Scholar 

  • Sanchez-Serrano, J.J., Keil, M., O ’Connor, A. et al. (1987) Wound-induced expression of a potato proteinase inhibitor II gene in transgenic plants. EMBO J., 6, 303–6.

    CAS  Google Scholar 

  • Saravitz, D.M., Pharr, D.M. and Carter, T.E. (1987) Galactrinol synthase activity and soluble sugars in developing seeds of four soybean genotypes. Plant Physiol., 83, 185–9.

    Article  CAS  Google Scholar 

  • Satoh, H. and Omura, T. (1981) New endosperm mutations induced by chemical mutagens in rice, Oryza sativa. Jpn J. Breeding, 31, 316–26.

    CAS  Google Scholar 

  • Schell, J. (1987) Transgenic plants as tools to study the molecular organization of plant genes. Science, 237, 1176–83.

    Article  Google Scholar 

  • Schmidt, R.J., Burr, F.A. and Burr, B. (1987) Transposon tagging and molecular analysis of the maize regulatory locus opaque-2. Science, 238, 960–3.

    Article  CAS  Google Scholar 

  • Schmidt, R.J., Burr, F.A., Aukerman, M.J. and Burr, B. (1990) Maize regulatory gene opaque-2 encodes a protein with ’leucine-zipper’ motif that binds to zein DNA. Proc. Natl Acad. Sci. USA, 87, 46–50.

    Article  CAS  Google Scholar 

  • Sheldon, E., Feri, R., Fedoroff, N. and Hannah, L.C (1983) Isolation and analysis of a genomic clone encoding sucrose synthetase in maize: evidence for two introns in Sh. Mol. Gen. Genet., 190, 421–6.

    Article  CAS  Google Scholar 

  • Shewry, P.R. and Miflin, B.J. (1984) Seed storage proteins of economically important cereals. Adv. Cereal Sci. Technol., 7, 1–83.

    Google Scholar 

  • Shewry, P.R., Halford, N.G and Tatham, A. (1989) The high molecular weight subunits of wheat, barley, and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. Oxford Surv. Plant Mol. Cell. Biol., 6, 163–219.

    CAS  Google Scholar 

  • Shimamoto, K., Terada, R., Izawa, T. and Fujimoto, H. (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature, 337, 274–6.

    Article  Google Scholar 

  • Singh, R. and Axtell, J.D. (1973) High lysine mutant gene (hi) that improves protein quality and biological value of grain sorghum, Crop Sci., 13, 535–9.

    Article  CAS  Google Scholar 

  • Slabas, A.R., Harding, P. et al. (1987) Oil seed acyl carrier protein (ACP) protein and gene structure, in The Metabolism Structure, and Function of Plant Lipids, (eds P.K. Stumpf, J.B. Mudd and W.D. Ness), Plenum Press, New York, pp. 697–700.

    Google Scholar 

  • Slightom, J.L., Drong, R.F., Klassy, R.C. and Hoffmann, L.M. (1985) Nucleotide sequences from phaseolin cDNA clones: the major storage protein from Phaseolus vulgaris are encoded bv two unique gene families. Nucl. Acids Res., 13, 6483–98.

    Article  CAS  Google Scholar 

  • Slightom, J.S., Sun, S.M. and Hall, T.C. (1983) Complete nucleotide sequence of a French bean storage protein gene: phaseolin. Proc. Natl Acad. Sci., 80, 1897–901.

    Article  CAS  Google Scholar 

  • Soave, C. and Salamini, F. (1984) Organization and regulation of zein genes in maize endosperm. Phil. Trans. R. Soc. Lond., (B) 304, 341–7.

    Article  CAS  Google Scholar 

  • Soave, C, Tardani, L., di Fonzo, N. and Salamini, F. (1981) Zein level in maize endosperm depends on a protein under control of the opaque-2 and opaque-6 loci. Cell, 27, 403–10.

    Article  CAS  Google Scholar 

  • Somerville, C.R., McCourt, P., Kunst, L. and Browse, J. (1987) Mutants of Arabidopsis deficient in fatty acid desaturation, in The Metabolism, Structure and Function of Plant Lipids (eds P.K. Stumpf, J.B. Mudd and W.D. Ness), Plenum Press, New York, pp. 669–76.

    Google Scholar 

  • Soya Bluebook (1982) American Soybean Association, St Louis, MO.

    Google Scholar 

  • Spencer, D. and Higgins, T.J.V. (1982) Seed maturation and deposition of storage proteins, in Molecular Biology of Plant Cells, (ed. H. Smith), Botanical Monographs, Vol. 14, 306–36.

    Google Scholar 

  • Start, W.G., Ma, Y., Polacco, J.C. et al. (1986) Two soybean seed lipoxygenase nulls accumulate reduced levels of lipoxygenase transcripts. Plant Mol. Biol., 7, 11–23.

    Article  CAS  Google Scholar 

  • Sun, S.M., Slightom, J.L. and Hall, T.C. (1981) Intervening sequences in a plant gene: comparison of the partial sequence of cDNA and genomic DNA of French bean phaseolin. Nature, 289, 37–41.

    Article  CAS  Google Scholar 

  • Takaiwa, F., Kikuchi, S. and Oono, K. (1986) The structure of rice storage glutelin precursor deduced from cDNA. FEBS Lett., 206, 33–5.

    Article  CAS  Google Scholar 

  • Takaiwa, F., Kikuchi, S. and Oono, K. (1987a). A rice glutelin gene family — a major type of glutelin mRNAs can be divided into two classes. Mol. Gen. Genet., 208, 15–22.

    Article  CAS  Google Scholar 

  • Takaiwa, F., Ebinuma, H., Kikuchi, S. and Oono, K. (1987b) Nuleotide sequence of a rice glutelin gene. FEBS Lett., 221, 43–7.

    Article  CAS  Google Scholar 

  • Talbot, D.R., Adang, M.J., Slightom, J.L. and Hall, T.C. (1984) Size and organization of a multigene family encoding phaseolin, the major seed storage protein of Phaseolus vulgaris. Mol. Gen. Genet., 198, 42–9.

    Article  CAS  Google Scholar 

  • Taliercio, E.W. and Chourey, P.S. (1989) Post-transcriptional control of sucrose synthase expression in anaerobic seedlings of maize. Plant Physiol., 90, 1359–64.

    Article  CAS  Google Scholar 

  • Thomburg, R.W., An, G., Cleveland, T.E. et al. (1987) Wound inducible expression of a potato inhibitor I-chloramphenical acetyl transferase gene fusion in transgenic tobacco plants. Proc. Natl Acad. Sci. USA, 84, 744–8.

    Article  Google Scholar 

  • Toenniessen, G.H. (1985) Plant molecular biology and the international agricultural research system. Plant Mol. Biol. Rep., 3, 1–11.

    Article  Google Scholar 

  • Topfer, R., Gronenbom, B. et al. (1989) Uptake and transient expression of chimeric genes in seed derived embryos. Plant Cell, 1, 133–9.

    Article  CAS  Google Scholar 

  • Tsai, C.Y. and Nelson, O.E. (1966) Starch deficient maize mutants lacking ADP- glucose pyrophosphorylase activity. Science, 151, 341–3.

    Article  CAS  Google Scholar 

  • Uchimiya, H., Fushimi, T. et al. (1986) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa). Mol Gen. Genet., 204, 204–7.

    Article  CAS  Google Scholar 

  • Uchimiya, H., Handa, T. and Brar, D.S. (1989) Transgenic plants. J. Biotechnol., 12, 1–20.

    CAS  Google Scholar 

  • Villegas, E., Ortega, E. and Bauer, R. (1984) Chemical Methods Used at CIMMYT for Determining Protein Quality, CIMMYT, Mexico.

    Google Scholar 

  • Viotti, A., Abildsten, D., Pogna, N. et al. (1982) Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localization. EMBO]., 1, 53–8.

    CAS  Google Scholar 

  • Walling, L., Drews, G.N. and Goldberg, R.B. (1986) Transcriptional and posttranscriptional regulation of soybean seed protein mRNA levels. Proc. Natl Acad. Sci. USA, 83, 2123–7.

    Article  CAS  Google Scholar 

  • Weising, K., Schell, J. and Kahl, G. (1988) Foreign genes in plants: transfer, structure, expression and application. Annu. Rev. Genet., 22, 421–77.

    Article  CAS  Google Scholar 

  • Wienand, U. and Feix, G. (1980) Zein specific restriction enzyme fragments of maize DNA. FEBS Lett., 116,14–16.

    Article  CAS  Google Scholar 

  • Williamson, M.S., Forde, J., Buxton, B. and Kreis, M. (1987) Nucleotide sequence of barley chymotrypsin inhibitor-2 (CL-2) and its expression in normal and high-lysine barley. Eur. J. Biochem., 165, 99–106.

    Article  CAS  Google Scholar 

  • Wright, D.J. (1988) The seed globulins — Part II, in Development in Food Proteins, (ed.B.J.F. Hudson), Elsevier Applied Science Publishers, London, Vol. 6,119–77.

    Google Scholar 

  • Yang, M.S., Espinoza, N.O., Nagpala, P.G. et al. (1989) Expression of a synthetic gene for improved protein quality in transformed potato plants. Plant Sci., 64 99–111.

    Article  CAS  Google Scholar 

  • Yano, M., Okuno, K., Kawakami, J. et al. (1985) High amylose mutants of rice, Oryza sativa. Theor. Appi. Genet., 69, 253–7.

    Article  CAS  Google Scholar 

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de Lumen, B.O. (1996). Molecular approaches to improving the nutritional and functional properties of oilseeds, grains and legumes. In: Nwokolo, E., Smartt, J. (eds) Food and Feed from Legumes and Oilseeds. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0433-3_39

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