Proteins F1 And F2 of Streptococcus Pyogenes
Microbial adhesion to host tissues is the initial event in the pathogenesis of most infections and, as a such, is an attractive target for the development of new antimicrobial therapeutics. Fibronectin (Fn) is a multifunctional glycoprotein present in soluble form in plasma and other body fluids and in insoluble form in extracellular matrices (ECM) and basement membranes (Mosher, 1989; Hynes, 1990). Since it binds specifically to a variety of receptors and substrates molecules, many bacterial pathogens including staphylococci, Escherichia coli, Treponema pallidum, mycobacteria and streptococci have exploited the binding properties of Fn to acquire a mechanism for adherence, colonization and subsequent invasion of host tissues (Patti et al., 1994). Thus, an understanding of the molecular details of bacteria-Fn interactions will not only provide considerable insight to the pathogenesis of many bacterial diseases, but will also serve as a powerful model system for structural and functional analyses of Fn itself.
KeywordsRespiratory Epithelial Cell Fibronectin Binding Collagen Binding Domain Cell Wall Binding Domain Streptococcus Dysgalactiae
Unable to display preview. Download preview PDF.
- Hanski, E. Horwitz, P. A., and Caparon M. G. (1992). Expression of protein F, the fibronectin-binding protein of Streptococcus pyogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells. Infect. Immun. 60: 5119–5125.PubMedGoogle Scholar
- Hynes, R. O., (1990). Fibronectins. Springer-Verlag, Berlin.Google Scholar
- Jaffe, J., Natanson-Yaron, S., Caparon, M. G., and Hanski, E. (1996) Protein F2, a novel fibronectin binding protein from Streptococcus pyogenes, possesses two binding domains. Mol. Microbiol, (in press).Google Scholar
- Lindgren, P.-E., McGavin, M., Signäs, C., Guss, B., Gurusiddappa, S., Höök, M., and Lindberg, M. (1993) Two different genes coding for fibronectin-binding proteins from Streptococcus dysgalactiae: the complete nucleotide sequence and characterization of binding domains. Eur. J. Biochem. 214: 819–827.PubMedCrossRefGoogle Scholar
- McGavin, M. J., Gurusiddappa, S., Lindgren, P.-E., Lindberg, M., Raucci, G., and Höök, M. (1993). Fibronectin receptors from Streptococcus dysgalactiae Staphylococcus aureus. J. Biol Chem. 268: 23946–23953.Google Scholar
- Mosher, D. F., (1989). Fibronectin. Academic Press. New York.Google Scholar
- Ozeri, V., Tovi, A., Burstein, I., Natanson-Yaron, S., Caparon, M. G., Yamada, K. M., Akiyama, S. K., Vlodavsky, I., and Hanski, E. (1996) A Two-domain mechanism for Group A Streptococcal adherence through protein F to extracellular matrix. EMBO J., 15: (in press).Google Scholar