Abstract
Alfalfa [Medicago sativa L.,(Leguminosae)] is of interest as a fodder plant and grown on large areas in many world regions. In Uzbekistan alfalfa is widely cultivated in crop rotation with cotton and this makes the plant an important raw material. Alfalfa saponin is well known to influence animal organisms1,2, and to affect insect and fungus growth3. It was shown by Slotwinska4 that the saponins induced the reduction of mitotic index, viability, and growth of cells in L-cells culture. Study of the growth-retarding activity of alfalfa saponins on higher plants was by Waller, Jurzysta and Thorne5. Owing to this we have studied triterpene glycosides of this plant of great national economic significance. Objects of study were alfalfa roots and leaves. As a result of thin layer chromatography (TLC) there have been discovered 13 glycosides in the roots and 10 in the leaves. Qualitative compositions of glycosides from plant organs are different, and do not have identical components. The structures were established for 10 root glycosides6.
Keywords
- Medicagenic Acid
- Triterpene Glycoside
- Wheat Coleoptile
- Alfalfa Root
- Crude Saponin
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
G.Reshef, B.Gestetner, Y.Birk and A.Bondi, Effect of alfalfa saponins on the growth and lipid metabolism of mice and quails, J. Sci. Food Agric. 27:63 (1976).
M.R.Malinow, W.E.Connor, P.McLaughlin, C.Stafford, D.S.Lin, A.L.Livingston, G.O.Kohier and W.P.McNulty, Cholesterol and bile acid balance in Macaca fascicularis. Effect of alfalfa saponins, J. Clin. Invest. 67:156 (1981).
E.Horber, K.T.Leath, B.Berrang, V.Marcarian and C.H.Hanson, Biological activities of saponin components from DuPuits and Lahontan alfalfa, Entomol. Exp. Appl. 17:410 (1974).
M.Slotwinska, The effect of alfalfa saponins on L-cells culture in vitro, Ann. Univ. Mariae Curie—Sklodowska, Sect. C 38:177 (1983).
G. R.Waller, M.Jurzysta and R.L.Z.Thorne, Root saponins from alfalfa (Medicago sativa L.) and their allelopathic activity on weeds and wheat, Allelopathy J. 2:21 (1995).
A.E.Timbekova, A.S.Shashkov and N.K.Abubakirov, Triterpene glycosides of alfalfa. VII. Medicosides E and F, Khim. Prirod. Soedin. 701 (1993).
R.I.Morris, W.B.Dye and P.S.Gisler, Isolation, purification, and structural identity of an alfalfa root saponin, J. Org. Chem. 26:1241 (1960).
S.Hakomori, Rapid permethylation of glycolipids and polysaccharides, catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide, J. Biochem. 55:205 (1964).
G.Massiot, C.Lavaud, L.Le Men-Olivier, G.Van Binst, S.P.F.Miller and H.M.Fales, Structural elucidation of alfalfa root saponins by mass spectrometry and nuclear magnetic resonance analysis, J. Chem. Soc., Perkin Trans. I 3071 (1988).
G.Massiot, C.Lavaud, V.Besson, L.Le Men-Olivier and G.Van Binst, Saponins from aerial parts of alfalfa (Medicago sativa), J. Agric. Food Chem. 39:78 (1991).
W. Oleszek, K.R.Price, I.J.Colquhoun, M.Jurzysta, M.Proszynski and G.R.Fenwick, Isolation and identification of alfalfa (Medicago sativa L.) root saponins: their activity in relation to a fungal bioassay, J. Agric. Food Chem. 38:1810 (1990).
M.Abdel-Akher, J.K.Hamilton, R.Montgomery and F.Smith, A new procedure for the determination of the fine structure of polysaccharides, J. Am. Chem. Soc. 74:4970 (1952).
Y. Imakura, S.Kobayashi and A.Mima, Bitter phenyl propanoid glycosides from Campsis chinensis, Phytochemistry.24:139 (1985).
M.M. Benidze, O.D.Dzhikiya, T.A.Pkheidze, E.P.Kemertelidze and A.S.Shashkov, Steroid glycosides of the leaves of Jucca aloifolia, Khim. Prirod. Soedin. 537 (1987).
T.Murakami, M.Nagasawa, S.Urayama and T.Satake, New triterpenoid saponins in the rhizome and roots of Caulophyllum robustum, Yakugaku Zasshi 88:321 (1968).
H.Kizu, S.Kitayma, F.Nakatani, T.Tomimori and T.Namba, Studies on Nepalese crude drugs. III. On the saponins of Hedera nepalensis K.Koch., Chem. Pharm. Bull. 33:3324 (1985).
S.Tarikov, A.E.Timbekova, N.K.Abubakirov and R.K.Koblov, Growth regulating activity of triterpene glycosides isolated from alfalfa roots (Medicago sativa), Uzbek. Biol. Zh. 6:24(1988).
V.M.Boguslavskii, S.A.Burceva, A.E.Timbekova and N.K.Abubakirov, Antimicrobial activity of alfalfa root glycosides, in: Microbiologiya v Sel’skom Khozyaistve, G.V. Merenyuk, Ed., Shtiintsa, Kishinev (1991).
K.I.Bel’tyukova and M.S.Matyshevskaya, Metody Issledovaniya Vozbuditelei Bakterial’nykh Boleznei Rastenii, Naukova dumka, Kiev (1968).
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© 1996 Plenum Press, New York
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Timbekova, A.E., Isaev, M.I., Abubakirov, N.K. (1996). Chemistry and Biological Activity of Triterpenoid Glycosides from Medicago Sativa . In: Waller, G.R., Yamasaki, K. (eds) Saponins Used in Food and Agriculture. Advances in Experimental Medicine and Biology, vol 405. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0413-5_14
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DOI: https://doi.org/10.1007/978-1-4613-0413-5_14
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