Abstract
We examined regulation of the human erythropoietin (Epo) gene through the GATA sequence in the Epo promoter, and demonstrated that Hep3B and HepG2 cells express human GATA-2 (hGATA-2) mRNA and protein. Nuclear extracts of QT6 cells transfected with hGATA-1, 2 or 3 transcription factors revealed specific binding to the GATA element in the human Epo gene promoter by gel mobility shift assay. Transient transfection of Hep3B cells with hGATA-1, 2 or 3 demonstrated that each of these transcription factors significantly decreased the level of expression of Epo mRNA as assessed by a competitive polymerase chain reaction (PCR). Furthermore, transient transfection of Hep3B cells with hGATA-1, 2 and 3 and an Epo-reporter gene construct showed significant inhibition of the Epo promoter. We conclude that the hGATA-1,2 and 3 transcription factors specifically bind to the GATA element in the human Epo gene promoter and negatively regulate Epo gene expression.
Supported by grants-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan, the Uehara Memorial Foundation, the Yamanouchi Foundation, the Ichiro Kanehara Foundation and the Chugai Foundation.
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© 1996 Plenum Press, New York
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Imagawa, S., Yamamoto, M., Miura, Y. (1996). Gata Transcription Factors Negatively Regulate Erythropoietin Gene Expression. In: Abraham, N.G., Asano, S., Brittinger, G., Maestroni, G.J.M., Shadduck, R.K. (eds) Molecular Biology of Hematopoiesis 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0391-6_61
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DOI: https://doi.org/10.1007/978-1-4613-0391-6_61
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