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Quantitative RT-PCR of PML-RARα Transcripts in Acute Promyelocytic Leukemia

  • Harry Iland
  • Francisca Springall
  • Tao Zeng
  • Katrina Bradfield

Abstract

Detection of PML-RARα fusion transcripts in acute promyelocytic leukemia (APL) by RT-PCR is a useful adjunct to cytogenetic analysis at the time of diagnosis, and is the method of choice for detection of minimal residual disease. Since PML-RARα transcripts are frequently detectable after successful remission induction with all-trans retinoic acid (ATRA), we have established a competitive RT-PCR protocol for quantitation of PML-RARα transcripts which could be used to assess the effects of ATRA induction and subsequent chemotherapy consolidation. In addition to quantitation of the target transcript (PML-RARα), simultaneous quantitation of a second template (transferrin receptor transcripts) provides a control for the quality of RNA extraction and for the efficiency of cDNA synthesis, since the level of PML-RARα transcripts can be expressed relative to the level of transferrin receptor transcripts. For patients with APL, we anticipate that decisions regarding the requirement for additional chemotherapy, or the optimal timing of allogeneic transplantion, will be facilitated by the ability to detect progressive increases in fusion transcripts.

Keywords

Chronic Myeloid Leukemia Acute Promyelocytic Leukemia Minimal Residual Disease Transferrin Receptor Fusion Transcript 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • Harry Iland
    • 1
    • 2
  • Francisca Springall
    • 1
  • Tao Zeng
    • 1
  • Katrina Bradfield
    • 1
  1. 1.The Kanematsu LaboratoriesRoyal Prince Alfred HospitalCamperdownAustralia
  2. 2.Kanematsu LaboratoriesRoyal Prince Alfred HospitalCamperdownAustralia

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