Structures of Multimeric Proteins by NMR

  • G. Marius Clore
  • Angela M. Gronenborn

Abstract

Recent advances in NMR methodology, in particular the increase in resolution afforded by the advent of three- and four-dimensional NMR (see Clore & Gronenborn, 1991 for a review) have permitted the structures of proteins in the 15-25 kDa range to be solved in solution. In this paper we discuss the extension of these methods to multimeric proteins and illustrate these with regard to the solution structures of two dimers (interleukin-8; Clore et al., 1990; and human macrophage inflammatory protein 1β(3; Lodi et al., 1994) and a tetramer (the oligomerization domain of p53; Clore et al., 1994, 1995a,b)

Keywords

Amide Carboxylate Oligomerization Polypeptide Macromolecule 

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Notes

References

  1. Covell, D.G., Smythers, G.W., Gronenborn, A.M. & Clore, G.M. (1994 Protein Science 3, 2064–2072.PubMedCrossRefGoogle Scholar
  2. Clore, G.M. & Gronenborn, A.M. (1991) Science 252, 1390–1399.PubMedCrossRefGoogle Scholar
  3. Clore, G.M. & Gronenborn, A.M. (1994) Protein Science 3, 372–390.PubMedCrossRefGoogle Scholar
  4. Clore, G.M., Appella, E., Yamada, M., Matsushima, K. & Gronenborn, A.M. (1990) Biochemistry 29, 1689–1696.PubMedCrossRefGoogle Scholar
  5. Clore, G.M., Omichinski, J.G., Sakaguchi, K., Zambrano, N, Sakamoto, H., Appella, E. & Clore, G.M. (1994) Science 265, 386–391PubMedCrossRefGoogle Scholar
  6. Clore, G.M., Omichinski, J.G., Sakaguchi, K., Zambrano, N, Sakamoto, H., Appella, E. & Clore, G.M. (1995a) Science 267, 1515–1516PubMedCrossRefGoogle Scholar
  7. Clore, G.M., Ernst, J., Clubb, R., Omichinski, J.G., Kennedy, W.M.P, Sakaguchi, K., Appella, E.& Gronenborn, A.M. (1995b) Nature Struct. Biol. 2, 321–333.PubMedCrossRefGoogle Scholar
  8. Clubb, R.T., Omichinski, J.G., Sakaguchi, K., Appella, E., Gronenborn, A.M. & Clore, G.M. (1995) Protein Science 4, 855–862.PubMedCrossRefGoogle Scholar
  9. Jeffrey, P.D., Gonna, S. & Pavletich, N.P. (1995) Science 267, 1498–1501.PubMedCrossRefGoogle Scholar
  10. Lodi, P.J., Garrett, D.S., Kuszweski, J., Tsang, M.L.S., Weatherbee, J.A., Leonard, W.J., Gronenborn, A.M. & Clore, G.M. (1994) Science 263, 1762–1767.PubMedCrossRefGoogle Scholar
  11. Nilges, M. (1993)Proteins Struct. Funct. Genet. 17, 295–309Google Scholar

Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • G. Marius Clore
    • 1
  • Angela M. Gronenborn
    • 1
  1. 1.Laboratory of Chemical Physics National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of HealthBethesdaUSA

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