Molecular Organization of Endothelial Cell to Cell Junctions

  • Elisabetta Dejana
  • Jean-Luc Plantier
Part of the NATO ASI Series book series (NSSA, volume 281)


The endothelial monolayer forms the main barrier to macromolecular passage from blood to tissues and regulates the extravasation of circulating cells such as leukocytes. When endothelial permeability is altered such as in inflammatory conditions, edema takes place. Inflammatory edema is a significant component of many diseases e.g. adult respiratory syndrome, septicemia and hypoxia/reperfusion syndrome. This is frequently the result of both pressure-induced filtration of plasma proteins and changes in the permeability properties of the endothelial lining of the vascular wall (Majno et al., 1961).


Cell Junction Intercellular Junction Endothelial Permeability Endothelial Monolayer Adherence Junction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Albelda, S.M., Oliver, P., Romer, L., and Buck, C.A., 1990, EndoCAM: a novel endothelial cell-cell adhesion molecule. J. Cell. Biol. 110: 1227–1237.PubMedCrossRefGoogle Scholar
  2. Albelda, S.M., Muller, W.A., Buck, C.A., and Newman, P.J., 1991, Molecular and cellular properties of PECAM-1 (endoCAM/CD31): A novel vascular cell-cell adhesion molecule. J. Cell Biol. 114: 1059–1068.PubMedCrossRefGoogle Scholar
  3. Carter, W.G., Wayner, E.A., Bouchard, T.S., and Kaur, P., 1990, The role of integrins α3β1 and α3β1 in cell-cell and cell-substrate adhesion of human epidermal cells. J. Cell. Biol. 110: 1378–1404.CrossRefGoogle Scholar
  4. De Luca, M., Tamura, R.N., Kajiji, S., Bondanza, S., Rossino, P., Cancedda, R., Marchisio, P.C., and Quaranta, V., 1990, Polarized integrin mediates human keratynocyte adhesion to basal lamina. Proc. Natl. Acad. Sci. U.S.A. 87: 6888–6892.PubMedCrossRefGoogle Scholar
  5. Franke, W.W., Cowin, P., Grund, C., Kuhn, C., and Kepprell, H.P., 1988, The endothelial junction: the plaque and its components.In Simionescu N and Simionescu M (eds.) Endothelial Cell Biology in Health and Disease 147–166.Google Scholar
  6. Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, S., and Tsukita, S., 1993, Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell. Biol 123: 1777–1788.PubMedCrossRefGoogle Scholar
  7. Goyert, S.M., Ferrero, E.M., Seremetis, S.V., Winchester, R.J., Silver, J., and Mattison, A.C., 1986, Biochemistry and expression of myelomonocytic antigens. J. Immunol. 137: 3909–3914.PubMedGoogle Scholar
  8. Haselton, F.R., Alexander, J.S., Mueller, S.N., and Fishman, A.P., 1992, Modulation of endothelial paracellular permeability. A mechanistic approach.In Simionescu N and Simionescu M (eds.) Endothelial cell disfunctions 103–126.Google Scholar
  9. Hynes, R.O., 1992, Integrals: Versatility, modulation and signaling in cell adhesion. Cell 69: 11–25.PubMedCrossRefGoogle Scholar
  10. Joris, I., Cuénoud, H.F., Doern, G.V., Underwood, J.M., and Majno, G., 1992, Endothelial permeability in Inflammation. The role of capillaries versus venules. In Simionescu N and Simionescu M (eds.) Endothelial cell disfunctions 233–242.Google Scholar
  11. Lampugnani, M.G., Resnati, M., Dejana, E., and Marchisio, P.C., 1991, The role of integrins in the maintenance of endothelial monolayer integrity. J. Cell. Biol. 112: 479–490.PubMedCrossRefGoogle Scholar
  12. Lampugnani, M.G., Resnati, M., Raiteri, M., Pigott, R., Pisacane, A., Houen, G., Ruco, L.P., and Dejana, E., 1992, A novel endothelial specific memebrane protein is a marker of cell-cell contacts. J. Cell. Biol 119: 239–246.CrossRefGoogle Scholar
  13. Leach, L., Clark, P., Lampugnani, M.G., Arroyo, A.G., Dejana, E., and Firth, J.A., 1993, Immunoelectron characterization of the inter-endothelial junctions of human term placenta. J. Cell. Sci., in press.Google Scholar
  14. Liaw, C.W., Cannon, C., Power, M.D., Kiboneka,P.K., and Rubin,L.L., 1990, Identification and cloning of two species of Cadherins in bovine endothelial cells. EMBO J. 9: 2701–2708.Google Scholar
  15. Majno, G., Palade, G.E., and Schoefl, G.I., 1961, Studies on inflammation.II.The site of action of histamine and serotonin along the vascular tree: A topographic study. J. Biophys. Biochem. Cytol. 11: 607–626.PubMedCrossRefGoogle Scholar
  16. Miyatami, S., Shimamura, K., and Hatta, M. et al., 1989, Neural Cadherin: role in selective cell-cell adhesion. Science 245: 631–635.CrossRefGoogle Scholar
  17. Muller, W.A., Ratti, C.M., McDonnell, S.L., and Cohn, Z.A., 1989, A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular juntions. J. Exp. Med. 170: 399–414.PubMedCrossRefGoogle Scholar
  18. Muller, W.A., Weigl, S.A., Deng, X., and Philipps, D.M., 1993, PECAM-1 is required for transendothelial migration of leucocytes. J. Exp. Med. 178: 449–460.PubMedCrossRefGoogle Scholar
  19. Newman, P.J., Berndt, M.C., Gorski, J., White II, G.C., Lyman, S., Paddock, C., and Muller, W.A., 1990, PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219–1222.Google Scholar
  20. Nose, A., Nagafuchi, A., and Takeichi, M., 1988, Expressed recombinant Cadherins mediate cell sorting in model systems. Cell 54: 993–1001.PubMedCrossRefGoogle Scholar
  21. Ozawa, M., Baribault, H., and Kemler, R., 1989, The cytoplasmatic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species. EMBO J. 8: 1711–1717.PubMedGoogle Scholar
  22. Salomon, D., Ayalon, O., Patel-King, R., Hynes, R.O., and Geiger, B., 1992, Extrajunctional distribution of N-cadherin in cultured human endothelial cells. J. Cell. Sci. 102: 1–11.Google Scholar
  23. Schmelz, M. and Franke, W.W., 1993, Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell. Biol. 61: 274–289.PubMedGoogle Scholar
  24. Simionescu, N, 1991, Endothelial transport macromolecules: transcytosis and endocytosis. Cell Biol. Reviews 25: 5–80.Google Scholar
  25. Simmons, D.L.,Walker, C., Power, C.,and Pigott, R., 1990, Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen. J. Exp. Med. 171: 2147–2152.Google Scholar
  26. Stockinger, H., Gadd, S.J., Eher, R., Majdic, O., Schreiber, W., Kasinrerk, W., Strass, B., Schnabl, E., and Knapp, W., 1990, Molecular characterization and functional analysis of the leukocyte surface protein CD31. J. Immunol 145: 3889–3897.PubMedGoogle Scholar
  27. Takeda, T., Yamashita, Y., Shimazaki, S., and Mitsui, Y., 1992, Histamine decreases the permeability of an endothelial cell monolayer by stimulating cyclic AMP production through the H2-receptor. J. Cell. Sci. 101: 745–750.PubMedGoogle Scholar
  28. Takeichi, M., 1991, Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251: 1451–1455.PubMedCrossRefGoogle Scholar
  29. Tanaka, Y., Albelda, S.M., Horgan, K.J., van Seventer, G.A., Shimizu, Y., Newman, W., Hallan, J., Newman, P. J., Buck, C.A., and Shaw, S., 1992, CD31 expressed on distinctive T cell subsets is a preferential amplifier of betal integrin-mediated adhesion. J. Exp. Med. 176: 245–253.PubMedCrossRefGoogle Scholar
  30. Tsukita, S., Tsukita, S., Nagafichi, A., and Yonemura, S., 1992, Molecular linkage between Cadherins and actin filaments in cell-cell adherens junctions. Curr. Opin. Cell. Biol 4: 834–839.PubMedCrossRefGoogle Scholar
  31. Vaporcyan, A.A, DeLisser, H.M., Yan, H.C., Mendiguren, I.I., Thorm, S.R., Jones, M.L., Ward, P.A. and Albelda, S.M., 1993, Involvement of platelet-endothelial cell adhesion molecule-1 in neutrophil recruitment in vivo. Science 262: 1580–1582.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1996

Authors and Affiliations

  • Elisabetta Dejana
    • 1
  • Jean-Luc Plantier
    • 1
  1. 1.CEA Departement de Biologie Moleculaire et StructuraleLaboratoire d’Hematologie, INSERM U217 CEN-GGrenobleFrance

Personalised recommendations