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Molecular Correlates of Chromosome Bands

  • Orlando J. Miller
  • Eeva Therman
Chapter

Abstract

Bernardi and his coworkers have demonstrated that the human genome is organized into alternating blocks, each over 300 kb long, of rather homogeneous DNA of quite different GC richness, called isochores (Saccone et al., 1996). These can be separated from one another on the basis of their buoyant density in a cesium chloride ultracentrifugal gradient (Fig. 7.1). This reflects the average nucleotide composition of the isochores, because GC base pairs and GC-rich DNA are denser than AT base pairs and AT-rich DNA. There are five classes of isochores, called Li, L2 (pooled in Fig. 7.1), H1, H2, and H3, in order of increasing buoyant density and thus GC-richness. In situ hybridization shows that the H3 isochores are gene-richest (especially in housekeeping genes) and are present in highest concentration in 28 T-bands and at somewhat lower concentration in 31 additional R-bands (Fig. 7.2; see color insert).

Keywords

Chromosome Band Molecular Correlate CCGG Site Color Insert Campomelic Dysplasia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Orlando J. Miller
    • 1
  • Eeva Therman
    • 2
  1. 1.Center for Molecular Medicine and GeneticsWayne State University School of MedicineDetroitUSA
  2. 2.Laboratory of GeneticsUniversity of WisconsinMadisonUSA

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