Stomatal Behavior of Forest Trees in Relation to Hydraulic, Chemical, and Environmental Factors

  • Robert M. Augé
Part of the Ecological Studies book series (ECOLSTUD, volume 166)


Stomata regulate plant carbon gain, water loss, and other physiological determinants of forest productivity. Our ability to assess impacts of environmental changes on forest ecosystems relies heavily, therefore, on understanding stomatal function and control. The recent discovery of nonhydraulic, root-sourced stress Signals is changing our understanding of how plants “sense” and how stomata respond to fluctuations in soil moisture. Formerly, it had been widely held that stomatal conductance (g s ) was hydraulically regulated by leaf water potential (Ψ) or turgor potential (Ψ p ) Kramer and Boyer 1995), at least in anisohydric plants (Tardieu et al. 1996; Tardieu and Simmoneau 1998). However, there are several instances in which g s was inhibited in drying soils even in the absence of perturbations in leaf water Status (Davies et al. 1994). Such studies suggest that stomatal closure resulting from soil-water depletion can be mediated by changes in root water Status through effects on the chemical flow of Information from root to shoot.


Stomatal Conductance Photosynthetic Photon Flux Density Plant Cell Environ Stomatal Response Stomatal Behavior 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Augé RM, Duan X (1991) Mycorrhizal fungi and nonhydraulic root Signals of soil drying. Plant Physiol 97:821–824.PubMedCrossRefGoogle Scholar
  2. Augé RM, Duan X, Croker JL, Witte WT, Green CD (1998) Foliar dehydration tolerance of twelve deciduous tree species. J Exp Bot 49:753–759.Google Scholar
  3. Augé RM, Duan X, Ebel C, Stodola AJW (1994) Nonhydraulic signaling of soil drying in mycorrhizal maize. Planta 193:74–82.CrossRefGoogle Scholar
  4. Augé RM, Green CD, Johnson TB, Stodola AJW, Saxton AM, Olinick JB, Evans RM (2000) Correlations of stomatal conductance with hydraulic and chemical factors in several deciduous free species in a natural habitat. New Phytol 145:483–500.CrossRefGoogle Scholar
  5. Augé RM, Moore JL (2002) Stomatal response to nonhydraulic root-to-shoot communication of partial soil drying in relation to foliar dehydration tolerance. Exp Environ Bot 47:217–229.CrossRefGoogle Scholar
  6. Augé RM, Stodola AJW (1990) An apparent increase in symplastic water contributes to greater turgor in mycorrhizal roots of droughted Rosa plants. New Phytol 115:285–295.CrossRefGoogle Scholar
  7. Bahari ZA, Pallardy SG, Parker WC (1985) Photosynthesis, water relations, and drought adaptation in six woody species of oak-hickory forests in central Missouri. Forest Sci 31:557–569.Google Scholar
  8. Ball J, Woodrow IE, Berry JA (1987) A model predicting stomatal conductance and its contribution to the control of photosynthesis under different environmental conditions. In Biggins J (Ed) Progress in photosynthesis research, Vol. 4. Proceedings of the Vllth International Congress on Photosynthesis, Providence, Rhode Island, Aug 10–15, 1986. Martinus Nijhoff Publishers, Dordrecht, Netherlands.Google Scholar
  9. Berkowitz AR, Canham CD, Kelly VR (1995) Competition vs facilitation of tree seedling growth and survival in early successional communities. Ecology 76:1156–1168.CrossRefGoogle Scholar
  10. Cornish K, Zeevaart JAD (1985) Abscisic acid aecumulation by roots of Xanthium strumarium L. and Lycopersicon esculentum Mill. in relation to water stress. Plant Physiol 79:653–658.PubMedCrossRefGoogle Scholar
  11. Correia M, Rodrigues M, Ferreira M, Pereira J (1997) Diurnal change in the relationship between stomatal conductance and abscisic acid in the xylem sap of field-grown peach trees. J Exp Bot 48:1727–1736.Google Scholar
  12. Croker JL, Witte WT, Augé RM (1998) Stomatal sensitivity to nonhydraulic root-to-shoot Signals of partial soil drying in six temperate, deciduous forest trees. J Exp Bot 49:761–774.Google Scholar
  13. Davies WJ, Tardieu F, Trejo CL (1994) How do chemical Signals work in plants that grow in drying soil?. Plant Physiol 104:309–314.PubMedGoogle Scholar
  14. Fußeder A, Wartinger A, Hartung W, Schulze ED, Heilmeier H (1992) Cytokinins in the xylem sap of desert-grown almond (Prunus dulcis) trees—Daily courses and their possible interactions with abscisic acid and leaf conductance. New Phytol 122:45–52.CrossRefGoogle Scholar
  15. Gowing DJG, Jones HG, Davies WJ (1993b) Xylem-transported abscisic acid—The relative importance of its mass and its concentration in the control of stomatal aperture. Plant Cell Environ 16:453–459.CrossRefGoogle Scholar
  16. Gowing DJG, Davies WJ, Jones HG (1990) A positive root-sourced signal as an indicator of soil drying in apple, Malus X domestica Borkh. J Exp Bot 41:1535–1540.CrossRefGoogle Scholar
  17. Graves WR (1994) Seedling development of sugar maple and black maple irrigated at various frequencies. Hort Sci 119:1292–1294.Google Scholar
  18. Hansen H, Dorffling K (1999) Changes of free and conjugated abscisic acid and phaseic acid in xylem sap of drought-stressed sunflower plants. J Exp Bot 50:1599–1605.Google Scholar
  19. Hanson PJ, Todd DE, Amithor JS (2001) A six-year study of sapling and large-tree growth and mortality responses to natural and induced variability in preeipitation and throughfall. Tree Physiol 21:345–358.PubMedCrossRefGoogle Scholar
  20. Hanson PJ, Todd DE, Huston MA, Joslin JD, Croker JL, Augé RM (1998) Description and field Performance of the Walker Branch Throughfall Displacement Experiment: 1993–1996. ORNL/TM-13586. Oak Ridge National Laboratory, Oak Ridge, Tennessee.CrossRefGoogle Scholar
  21. Hanson PJ, Todd DE, Edwards NT, Huston MA (1995) Field Performance of the Walker Branch Throughfall Displacement Experiment. In Jenkins A, Ferrier RC, Kirby C (Eds) Ecosystem manipulation experiments: Scientific approaches, experimental design and relevant results. Ecosystem Research Report #20. Commission of the European Communities, Brüssels, Belgium, pp 307–313.Google Scholar
  22. Hartung W, Radin JW (1989) Abscisic acid in the mesophyll apoplast and in the root xylem sap of water-stressed plants: The significance of pH gradients. In Randall DD, Blevins DG (Eds) Current topics in plant biochemistry and physiology. University of Missouri, Columbia, Missouri.Google Scholar
  23. Hartung W, Radin JW, Hendrix DL (1988) Abscisic acid movement into the apoplastic Solution of water-stressed cotton leaves. Plant Physiol 86:908–913.PubMedCrossRefGoogle Scholar
  24. Hartung W, Wilkinson S, Davies W (1998) Factors that regulate abscisic acid concentrations at the primary site of action at the guard cell. J Exp Bot 49:361–367.Google Scholar
  25. Heckenberger U, Schurr U, Schulze E (1996) Stomatal response to abscisic acid fed into the xylem of intact Helianthus annus (L.) plants. J Exp Bot 47:1405–1412.CrossRefGoogle Scholar
  26. Holbrook NM, Shashidhar VR, James RA, Munns R (2002) Stomatal control in tomato with ABA-deficient roots: response of grafted plants to soil drying. J Exp Bot 2002:1503–1514.CrossRefGoogle Scholar
  27. Jackson GE, Irvine J, Grace J, Khalil AMM (1995) Abscisic acid concentrations and fluxes in droughted conifer saphngs. Plant Cell Environ 18:13–22.CrossRefGoogle Scholar
  28. Jia W, Zhang J (1997) Comparison of exportation and metabolism of xylem-delivered ABA in maize leaves at different water Status and xylem sap pH. Plant Growth Reg 21:43–49.CrossRefGoogle Scholar
  29. Jia W, Zhang J, Zhang DP (1996) Metabolism of xylem-delivered ABA in relation to ABA flux and concentration in leaves of maize on Commelina communis. J Exp Bot 47:1085–1091.CrossRefGoogle Scholar
  30. Jones HG (1990a) Control of growth and stomatal behavior at the whole plant level: Effects of soil drying. In Davies WJ, Jeffcoat B (Eds) Importance of root to shoot communication in the responses to environmental stress. Mongraph No.l. British Society for Plant Growth Regulation, University of Bristol, England.Google Scholar
  31. Jones HG (1990b) Physiological aspects of the control of water Status in horticultural crops. Hort Science 25:19–26.Google Scholar
  32. Jones RJ, Mansfield TA (1970) Suppression of stomatal opening in leaves treated with abscisic acid. J Exp Bot 21: 714–719.CrossRefGoogle Scholar
  33. Khalil AAM, Grace J (1993) Does xylem sap ABA control the stomatal behavior of water stressed sycamore Acer pseudoplatanus L.) seedlings? J Exp Bot 44:1127–1134.CrossRefGoogle Scholar
  34. Knapp AK, Smith WK (1990) Stomatal and photosynthetic responses to variable sunlight. Physiol Plant 78:160–165.CrossRefGoogle Scholar
  35. Kramer PJ, Boyer JS (1995) Water relations of plants and soils. Academic Press, San Diego, California.Google Scholar
  36. Liang J, Zhang J, Wong M (1996) Stomatal conductance in relation to xylem sap ABA concentrations in two tropical trees, Acacia confusa and Litsea glutinosa. Plant Cell Environ 19:93–100.CrossRefGoogle Scholar
  37. Liang J, Zhang J, Wong M (1997) How do roots control xylem sap ABA concentration in response to soil drying?. Plant Cell Physiol 38:10–16.CrossRefGoogle Scholar
  38. Liu L, McDonald AJS, Stadenberg I, Davies WJ (2001a) Abscisic acid in leaves and roots of willow: Significance for stomatal conductance. Tree Physiol 21:759–764.PubMedCrossRefGoogle Scholar
  39. Liu L, McDonald AJS, Stadenberg I, Davies WJ (2001b) Stomatal and growth responses to partial drying of root tips in willow. Tree Physiol 21:765–770.PubMedCrossRefGoogle Scholar
  40. Loewenstein NJ, Pallardy SG (1998a) Drought tolerance, xylem sap abscisic acid and stomatal conductance during soil drying: A comparison of young plants of four temperate deciduous angiosperms. Tree Physiol 18:421–430.PubMedCrossRefGoogle Scholar
  41. Loewenstein NJ, Pallardy SG (1998b) Drought tolerance, xylem sap abscisic acid and stomatal conductance during soil drying: A comparison of canopy trees of three temperate deciduous angiosperms. Tree Physiol 18:431–439.PubMedCrossRefGoogle Scholar
  42. Loveys BR (1984) Diurnal changes in water relations and abscisic acid in field-grown Vitis vinifera cultivars. III. The influence of xylem-derived abscisic acid on leaf gas exchange. New Phytol 98:563–573.CrossRefGoogle Scholar
  43. Ludlow MM (1989) Strategies in response to water stress. In Kreeb HK, Richter H, Hinckley TM (Eds) Structural and functional responses to environmental stresses: Water shortage. SBP Academic Publishing, The Hague, The Netherlands.Google Scholar
  44. Ludlow MM, Sommer KJ, Flower DJ, Ferraris R, So HB (1989) Influence of root signals resulting from soil dehydration and high soil strength on the growth of crop plants. Curr Top Plant Biochem Physiol 8:81–99.Google Scholar
  45. Mansfield TA, McAinsh MR (1995) Hormones as regulators of water balance. In Davies PJ (Ed) Plant hormones. Kluwer Academic Publishers, The Hague, The Netherlands.Google Scholar
  46. Meyer BS, Anderson DB (1952) Plant physiology. D. Van Nostrand & Co., New York.Google Scholar
  47. Neuman DS, Rood SB, Smit BA (1990) Does cytokinin transport from root-to-shoot in the xylem sap regulate leaf responses to root hypoxia?. J Exp Bot. 41:1325–1333.CrossRefGoogle Scholar
  48. Netting AG (2000) pH, abscisic acid and the Integration of metabolism in plants under stressed and non-stressed conditions: Cellular responses to stress and their implication for plant water relations. J Exp Bot 51:147–158.PubMedCrossRefGoogle Scholar
  49. Pallardy SG, Rhoads JJ (1993) Morphological adaptations to drought in seedlings of deciduous angiosperms. Can J Forest Res 23:1766–1774.CrossRefGoogle Scholar
  50. Patonnier M, Peltier J, Marigo G (1999) Drought-induced increase in xylem malate and mannitol concentrations and closure of Fraxinus excelsior L. stomata. J Exp Bot 50:1223–1231.Google Scholar
  51. Pereira JS, Kozlowski TT (1977) Influence of light intensity, temperature and leaf area on stomatal aperture and water potential of woody plants. Can J Forest Res 7:145–153.CrossRefGoogle Scholar
  52. Saliendra NZ, Sperry JS, Comstock JP (1995) Influence of leaf eater Status on stomatal response to humidity, hydraulic conductance, and soil drought in Betula occidentalis. Planta 196:357–366.CrossRefGoogle Scholar
  53. Sauter A, Härtung W (2000) Radial transport of abscisic acid conjugates in maize roots: Its implication for long distance stress signals. J Exp Bot 51:929–935.PubMedCrossRefGoogle Scholar
  54. Schulze ED (1986) Whole-plant responses to drought. Aust J Plant Physiol 13:127–141.CrossRefGoogle Scholar
  55. Shumway DL, Steiner KC, Kolb TE (1993) Variation in seedling hydraulic architecture as a function of species and environment. Tree Physiol 12:41–54.PubMedGoogle Scholar
  56. Smith WK, Hollinger DY (1991) Measuring stomatal behavior. In Lassoie JP, Hinckley TM (Eds) Techniques and approaches in forest tree ecophysiology. CRC Press, Boca Raton, Florida.Google Scholar
  57. Socias X, Correia M, Chaves M, Medrano H (1997) The role of abscisic acid and water relations in drought responses of subterranean clover. J Exp Bot 48:1281–1288.CrossRefGoogle Scholar
  58. Tardieu F, Davies WJ (1993) Integration of hydraulic and chemical signalling in the control of stomatal conductance and water Status of droughted plants. Plant Cell Environ 16:341–349.CrossRefGoogle Scholar
  59. Tardieu F, Simonneau Th (1998) Variability among species of stomatal control under fluctuating soil water Status and evaporative demand: Modelling isohydric and anisohydric behaviors. J Exp Bot 49:419–432.Google Scholar
  60. Tardieu F, Katerji N, Benthenod O, Zhang J, Davies WJ (1991) Maize stomatal conductance in the field: Its relationship with soil and plant water potentials, mechanical constraints and ABA concentration in the xylem sap. Plant Cell Environ 14:121–126.CrossRefGoogle Scholar
  61. Tardieu F, Zhang J, Katerji N, Bethenod O, Palmer S, Davies WJ (1992) Xylem ABA controls the stomatal conductance of field-grown maize subjected to soil compaction or soil drying. Plant Cell Environ 15:193–197.CrossRefGoogle Scholar
  62. Tardieu F, Zhang J, Gowing DJG (1993) Stomatal control by both [ABA] in the xylem sap and leaf water Status: A test of a model for droughted or ABA-fed field-grown maize. Plant Cell Environ 16:413–420.CrossRefGoogle Scholar
  63. Tardieu F, LaFarge T, Simonneau T (1996) Stomatal control by fed or endogenous ABA in sunflower: Interpretation of correlations between leaf water potential and stomatal conductance in anisohydric species. Plant Cell Environ 19:75–84.CrossRefGoogle Scholar
  64. Thomas D, Eamus D (1999) The influence of predawn leaf water potential on stomatal responses to atmospheric water content at constant Ci and on stem hydraulic conductance and foliar ABA concentrations. J Exp Bot 50: 243–251.Google Scholar
  65. Thompson DS, Wilkinson S, Bacon MA, Davies WJ (1997) Multiple Signals and mechanisms that regulate leaf growth and stomatal behavior during water deficit. Physiol Plant 100:303–313.CrossRefGoogle Scholar
  66. Timbal J, Lefebvre C (1995) Seasonal changes in water potential and the growth of young Quercus rubra and Querem palustris plants during soil drought. Ann Sci For 52:67–79.CrossRefGoogle Scholar
  67. Triboulot MB, Fauveau ML, Breda N, Label P, Dreyer E (1996) Stomatal conductance and xylem-sap abscisic acid (ABA) in adult oak trees during a gradually imposed drought. Ann Sci For 53:207–220.CrossRefGoogle Scholar
  68. Turner NC, Schulze ED, Gollan T (1984) The responses of stomata to vapour pressure deficits and soil water content. I. Species comparison at high soil water Contents. Oecologia 63:338–342.CrossRefGoogle Scholar
  69. Viven P, Aussen G, Levy G (1993) Difference in drought resistance among three deciduous oak species grown in large boxes. Ann Sci For 50:221–233.CrossRefGoogle Scholar
  70. Wartinger A, Heilmeier H, Hartung W, Schulze D (1990) Daily and seasonal courses of leaf conductance and abscisic acid in the xylem sap of almond trees [Prunus dulcis (Miller) DA Webb] under desert conditions. New Phytol 116:581–587.CrossRefGoogle Scholar
  71. Wilkinson S, Corlett JE, Oger L, Davies WJ (1998) Effects of xylem pH on transpiration from wild-type and flacca mutant tomato leaves: A vital role for abscisic acid in preventing excessive water loss even from well-watered plants. Plant Physiol 117:703–709.PubMedCrossRefGoogle Scholar
  72. Wilson CC (1948) The effect of some environmental factors on the movements of guard cells. Plant Physiol 23: 5–37.PubMedCrossRefGoogle Scholar
  73. Wuenscher JE, Kozlowski TT (1971) The response of transpiration resistance to leaf temperature as a desiccation resistance mechanism in tree seedlings. Physiol Plant 24:254–259.CrossRefGoogle Scholar
  74. Zhang J, Davies WJ (1987) Increased synthesis of ABA in drying root tips and root-shoot communication via the transpiration stream. J Exp Bot 38:2015–2023.CrossRefGoogle Scholar
  75. Zhang J, Davies WJ (1989a) Abscisic acid produced in dehydrating roots may enable the plant to measure the water Status of the soil. Plant Cell Environ 12:73–81.CrossRefGoogle Scholar
  76. Zhang J, Davies WJ (1989b) Sequential response of whole plant water relations to prolonged soil drying and the involvement of xylem sap ABA in the regulation of stomatal behavior of sunflower plants. New Phytol 113: 167–174.CrossRefGoogle Scholar
  77. Zhang J, Davies WJ (1990a) Changes in the concentration of ABA in xylem sap as a funetion of changing soil water Status can aecount for changes in leaf conductance and growth. Plant Cell Environ 13:277–285.CrossRefGoogle Scholar
  78. Zhang J, Davies WJ (1990b) Does ABA in the xylem control the rate of leaf growth in soil-dried maize and sunflower plants?. J Exp Bot 41:1125–1132.CrossRefGoogle Scholar
  79. Zhang J, Schurr U, Davies WJ (1987) Control of stomatal behavior by abscisic acid which apparently originates in the roots. J Exp Bot 38:1174–1181.CrossRefGoogle Scholar
  80. Zhang J, Jia W, Zhang D-P (1997) Effect of leaf water Status and xylem pH on metabolism of xylem-transported abscisic acid. Plant Growth Reg 21:51–58.CrossRefGoogle Scholar

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© Springer Science+Business Media New York 2003

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  • Robert M. Augé

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