In Vivo Colony Forming Unit Population Sizes in Hypertransfused Sl/Sld Mice
As a result of a genetic defect expressed in the stroma of the tissues supporting hematopoiesis rather than in the hematopoietic cells themselves, mice of genotype Sl/Sl d (Steel, Steel-Dickie mutant mice) suffer a chronic macrocytic anemia, and are extremely sensitive to ionizing radiation (11). Previously, it was hypothesized that the genetic defect disturbs erythropoiesis very early in the erythron, perhaps at the point of commitment of in vivo colony-forming units (CFU) to the erythrocytic cellular line of differentiation (8, 19). In an earlier study (9), this hypothesis was tested by measuring and comparing, in Sl/Sl d mice and their congenic +/+ littermates, population sizes of high self-renewal potential and low self-renewal potential CFU. It was reasoned that a block in stem cell differentiation occurring early in the erythron would result in a deficiency of the latter but not of the former. However, our study did not bear this hypothesis out. Rather, it led to the unexpected observation that all the stem cell populations in Sl/Sl d mice, with the exception of the splenic CFU population, were reduced in size.
KeywordsStem Cell Differentiation Bone Marrow Stem Cell Separate Determination Macrocytic Anemia Haemopoietic Stem Cell
Unable to display preview. Download preview PDF.
- (5).Harrison, D. E., Malath, V. G., and Silber, R. Elevated erythrocyte nucleoside deaminase levels in genetically anemic W/Wv and Sl/Sld mice. Blood Cells, 1: 605, 1975.Google Scholar
- (9).McCarthy, K. F., Ledney, G. D., and Mitchell, R. A deficiency of hematopoietic stem cells in steel mice. Cell Tissue Kinet., in press, 1976.Google Scholar
- (11).Russell, E. S., and Bernstein, S. E. Blood and blood formation. In Green, E. L., ed., Biology of the Laboratory Mouse. New York: McGraw-Hill, 1966, 2nd ed., p. 315.Google Scholar
- (12).Schooley, J. C. as quoted in Harrison and Russell 1972.Google Scholar
- (13).Schooley, J. C. Studies of stem cell kinetics utilizing the spleen colony technique and the erythropoietin sensitivity test. In Bond, U. P., and Sugahara, T., eds., Comparative Cellular and Species Radiosensitivity. Tokyo: Igaku Shoin Ltd., 1969.Google Scholar
- (19).Wolf, N. S. Dissecting the hematopoietic microenvironment. I. Stem cell lodgment and commitment, and the proliferation and differentiation of erythropoietic descendants in the Sl/Sld mouse. Cell Tissue Kinet., 7: 89, 1974.Google Scholar