The Regulation by Carbon Dioxide of Carbohydrate and Lipid Metabolism in Isolated Perfused Tissue

  • William J. Longmore
  • Amy M. Liang
  • Michael L. McDaniel
Part of the Topics In Environmental Physiology And Medicine book series (TEPHY)


In 1960 Hastings and Dowdle reported that increasing medium bicarbonate concentration in the physiological range from approximately 10 mM to 40 mM, while maintaining pH at 7.40, caused glycogen synthesis from glucose in rat liver slices to increase 60% (HASTINGS and DOUDLE [1960]). In further studies it was found that fructose (HASTINGS and LONGMORE [1965]) and especially glycerol (LONGMORE, HASTINGS, and MAHOWALD [1964]) also served as substrates to produce the rise in glycogen synthesis as total CO2 concentration of the medium was increased, while pyruvate did not (HASTINGS and LONGMORE [1965]). In addition to the effect of CO2 on glycogen synthesis, it was observed that acetate-1-14C and pyruvate-2-14C incorporation into triglyceride and phospholipid fatty acids was increased an average of fourfold in rat liver slices when medium total CO2 concentration was increased from 10 mM to 40 mM at pH 7.40 (LONGMORE, et al. [1967]; LONGMORE [1968]). Bicarbonate has been shown by various workers to affect the activity of several enzymes in vitro, including glucose-6-phos- phate dehydrogenase, glucose-6-phosphate phosphohydrolase, phosphoenolpyruvate carboxykinase, glyceraldehyde phosphate de-hydrogenase, and aconitase (BANDURSKI and LIPMANN [1956]; CHANCE and PARKE [1967]; DICKMAN and CLOUTIER [1951]; DYSON, ANDERSON, and NORDLIE [1969]; LEVY [1963]; ANDERSON and NORDLIE [1968]; ANDERSON, HORNE, and NORDLIE [1968]; MCDANIEL and LONGMORE [1971]). The last two, glyceraldehyde phosphate dehydrogenase and aconitase, assume importance in the present report.


Fatty Acid Synthesis Glycogen Synthesis Phospholipid Fatty Acid Bicarbonate Concentration Mmole Glucose 
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Copyright information

© Springer-Verlag New York Inc. 1974

Authors and Affiliations

  • William J. Longmore
    • 1
  • Amy M. Liang
    • 1
  • Michael L. McDaniel
    • 1
  1. 1.Department of BiochemistrySt. Louis University School of MedicineSt. LouisUSA

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