Advertisement

Heterogeneity of Gut Glucagon-Like Immunoreactivity (GLI)

  • A. J. Moody
  • H. Jacobsen
  • F. Sundby
  • E. K. Frandsen
  • D. Baetens
  • L. Orci

Abstract

One component of gut glucagon-like immunoreactivity (GLI-1) has been purified and characterized. It is proposed that gut GLIs of a given species contain the full homologous glucagon sequence and are immunologically related to the glucagon precursors found in pancreatic A cells. The essential difference between the gastrointestinal cells which produce GLIs and the pancreatic A cells is the degree to which these cells shorten a common primary gene product before storage and secretion.

Keywords

Specific Antiserum Cyanogen Bromide Porcine Intestine Gastrointestinal Cell Chymotryptic Digestion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Assan, R. and Slusher, N. (1972): Structure-function and structure- immunoreactivity relationships of the glucagon molecule and related synthetic peptides. Diabetes 21, 843–855.PubMedGoogle Scholar
  2. 2.
    Bromer, W. W., Boucher, M. E. and Koffenberger, J. E. Jr. (1971): Amino acid sequence of bovine glucagon. J. Biol. Chem. 246, 2822–2827.PubMedGoogle Scholar
  3. 3.
    Buchanan, K. D. (1976): The role of enteric hormones in glucose homeostasis. Horm. Metab. Res. 6 (Suppl.) 80–84.PubMedGoogle Scholar
  4. 4.
    Heding, L. G., Frandsen, E. K. and Jacobsen, H. (1976): Structure-function relationship: immunologic. Metabolism 25 (Suppl. 1) 1327–1329.PubMedCrossRefGoogle Scholar
  5. 5.
    Jacobsen, H., Demandt, A., Moody, A. J. and Sundby, F. (1976). Europ. J. Biochem. Submitted for publication.Google Scholar
  6. Moody, A. J., Frandsen, E. K., and Sundby, F. (1975): Fractionation of gut glucagon-like activities by isoelectric focusing in polyacrylamide gel. In: Progress in Isoelectric Focusing and Isotachophoresis. P. G. Righetti, Ed. North-Holland Publishing Co., pp. 179–191.Google Scholar
  7. 7.
    Sundby, F., Jacobsen, H. and Moody, A. J. (1976): Purification and characterization of a protein from porcine gut with glucagon-like immunoreactivity. Horm. Metab. Res. 8, 366–371.PubMedCrossRefGoogle Scholar
  8. 8.
    Tager, H. S. and Steiner, D. F. (1973): Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon. Proc. Nat. Acad. Sci. USA 70, 2321–2325.PubMedCrossRefGoogle Scholar
  9. 9.
    Valverde, I., Rigopoulou, D., Exton, J., Ohneda, A., Eisentraut, A. M. and Unger, R. H. (1968): Demonstration and characterization of a second fraction of glucagon-like immunoreactivity in jejunal extracts. Am. J. Med. Sci. 255, 415–420.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • A. J. Moody
    • 1
    • 2
  • H. Jacobsen
    • 1
    • 2
  • F. Sundby
    • 1
    • 2
  • E. K. Frandsen
    • 1
    • 2
  • D. Baetens
    • 1
    • 2
  • L. Orci
    • 1
    • 2
  1. 1.Novo Research InstituteCopenhagenDenmark
  2. 2.Institut d’Histologie et d’EmbryologieUniversité de GenèveGenèveSwitzerland

Personalised recommendations