Skip to main content
  • 106 Accesses

Abstract

The authors studied the release of glucagon from isolated pancreatic islets using thin section preparations, freeze-fracture techniques and radioimmunoassay. The evidence strongly suggests that exocytosis is a significant mechanism for glucagon release during arginine stimulation, but not for the release induced by calcium deprivation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bussolati, G., Capella, C., Vassalo, G. and Solcia, E. (1971): Histochemical and ultrastructural studies on pancreatic A cells. Evidence for glucagon and non-glucagon components of the alpha granule. Diabetologia 7., 181–188.

    Article  PubMed  CAS  Google Scholar 

  2. Erlandsen, S. L., Parsons, J. A., Burke, J. P., Redick, J. A., Van Orden, D. E. and Van Orden, L. S. (1975): A modification of the unlabeled antibody enzyme method using heterologous antisera for the light microscopic and ultrastructural localization of insulin, glucagon and growth hormone. J. Histochem. Cytochem. 23., 666–677.

    Article  PubMed  CAS  Google Scholar 

  3. Esterhuizen, A. C. and Howell, S. L. (1970): Ultrastructure of the A cells of cat islets of Langerhans following sympathetic stimulation of glucagon secretion. J. Cell Biol. 47, 593–598.

    Article  Google Scholar 

  4. Gerich, J. E., Charles, M. A. and Grodsky, G. M. (1974): Characterization of the effects of arginine and glucose on glucagon and insulin release from the perfused rat pancreas. J. Clin. Invest. 54, 833-841.

    Google Scholar 

  5. Gomez-Acebo, J., Parrilla, R. and Candela, J. L. R. (1968): Fine structure of the A and D cells of the rabbit endocrine pancreas in vivo and incubated in vitro. I. Mechanism of secretion of the A cells. J. Cell Biol. 36, 33-44.

    Google Scholar 

  6. Lacy, P. E. and Kostianovsky, M. (1967): Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16, 35–39.

    PubMed  CAS  Google Scholar 

  7. Lazarus, S. S., Shapiro, S. and Volk B. W. (1968): Secretory granule formation and release in rabbit pancreatic A cells. Diabetes 17., 152–160.

    Google Scholar 

  8. Leclercq-Meyer, V., Marchand, J. and Malaisse, W. J. (1976): The role of calcium in glucagon release. Interactions between glucose and calcium. Diabetologia 12., 531–538.

    Google Scholar 

  9. Leclercq-Meyer, V., Rebolledo, O., Marchand, J. and Malaisse, W. J. (1975): Glucagon release: paradoxical stimulation during calcium deprivation. Science 189, 897–899.

    Article  PubMed  CAS  Google Scholar 

  10. Machino, M., Onoe, T. and Sakuma, H. (1966): Electron microscopic observations on the islet alpha cells of the domestic fowl pancreas. Electron Micr. 15, 249–256.

    CAS  Google Scholar 

  11. Munger, B. L. (1962): The secretory cycle of the pancreatic islet a-cell An electron microscopic study of normal and synthalin-treated rabbits. Lab. Invest. H, 885–901.

    Google Scholar 

  12. Orci, L., Malaisse-Lagae, F., Ravazzola, M., Rouiller, D., Renold, A. E., Perrelet, A. and Unger, R. H. (1975): A morphological basis for intercellular communication between alpha and beta cells in the endocrine pancreas. J. Clin. Invest. 56, 1066-1070.

    Article  PubMed  CAS  Google Scholar 

  13. Orci, L., Stauffacher, W., Renold, A. E. and Rouiller, Ch. (1971): The ultrastructural aspect of A-cells of non-ketotic and ketotic diabetic animals: indications for stimulation and inhibition of glucagon production. In: Current Topics on Glucagon. M. Austoni, C. Scandarelli, G. Federspil and A. Trisotto, Eds. Cedam, Padova, pp. 15–30.

    Google Scholar 

  14. Pagliara, A. S., Stillings, S. N., Hover, B., Martin, D. M. and Matchinsky, F. M. (1974): Glucose modulation of amino acid-induced glucagon and insulin release in the isolated perfused rat pancreas. J. Clin. Invest. 54, 819–832.

    Google Scholar 

  15. Rhoten, W. B. (1973): The cellular response of saurian pancreatic islets to chronic insulin administration. Gen. Comp. End. 20., 474–489.

    Article  CAS  Google Scholar 

  16. Smith, P. H. (1975): Effects of selective partial pancreatectomy on pancreatic islet cell morphology in the Japanese quail. Gen. Comp. Endo. 26., 310–320.

    Article  Google Scholar 

  17. Unger, R. H. (1976): Diabetes and the alpha-cell. Diabetes 25., 136–151.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1977 Springer-Verlag New York Inc.

About this paper

Cite this paper

Carpentier, JL., Malaisse-Lagae, F., Müller, W., Orci, L. (1977). Morphologic Study of Glucagon Release. In: Foà, P.P., Bajaj, J.S., Foà, N.L. (eds) GLUCAGON: Its Role in Physiology and Clinical Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6366-1_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-6366-1_2

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-6368-5

  • Online ISBN: 978-1-4612-6366-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics