Glucagon-Like Materials in Porcine and Rat Intestinal Mucosa

  • J. M. Conlon
  • K. D. Buchanan
  • R. F. Murphy
Conference paper


Extracts of gastrointestinal tissue contain glucagon-like immunoreactive (GLI) materials with molecular weights varying between 2000 and 30,000. Some of them react with antisera specific for the C-terminal, others with antisera specific for the N-terminal portion of the glucagon molecule. In response to perfusion with glucose the serosal surface of rat small intestine releases increased amounts of some GLI fractions.


Antigenic Site Cyanogen Bromide Serosal Surface Porcine Pancreas Immunoaffinity Chromatography 
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  1. 1.
    Assan, R. and Slusher, N. (1972): Structure/function and structure/ immunoreactivity relationships of the glucagon molecule and related synthetic peptides. Diabetes 21, 843–855.PubMedGoogle Scholar
  2. 2.
    Conlon, J. M., Flanagan, R. W. J., Buchanan, K. D. and Murphy, R. F. (1976): Characterisation of complexes of glucagon-like immunoreactivity (GLI) with proteins from plasma and tissue extracts. Current Topics in Diabetes Research. Abstracts IX Congress of the International Diabetes Federation. New Delhi, October 31-November 5, 1976.Google Scholar
  3. 3.
    Markussen, J. and Sundby, F. (1970): Protides of the Biological Fluids (17th Colloquium, Bruges, 1969 ) ed. H. Peeters, Pergamon Press, Oxford, p. 471.Google Scholar
  4. 4.
    Moody, A. J. (1972): Gastrointestinal glucagon-like immunoreactivity. In: Glucagon, Molecular Physiology, Clinical and Therapeutic Implications, 1st ed., P. J. Lefebvre and R. H. Unger, eds., Pergamon Press, Oxford, p. 319.Google Scholar
  5. 5.
    Murphy, R. F. (1974): Immunoaffinity chromatography. Biochem. Soc. Trans. 2, 1298.Google Scholar
  6. 6.
    Murphy, R. F., Buchanan, K. D. and Elmore, D. T. (1973): Isolation of glucagon-like immunoreactivity of gut by affinity chromatography on antiglucagon antibodies coupled to Sepharose-4B. Biochem. Biophys. Acta 303, 118–127.PubMedGoogle Scholar
  7. 7.
    O’Connor, F. A., Gardner, M. L. G. and Buchanan, K. D. (1976): Method for studying the release of GLI from the isolated perfused rat small intestine. Submitted for publication.Google Scholar
  8. 8.
    Sasaki, H., Faloona, G. R. and Unger, R. H. (1974): Candidate hormones of the gut. XIV. Enteroglucagon. Gastroenterology 67, 746–748.PubMedGoogle Scholar
  9. 9.
    Tager, H. S. and Steiner, D. F. (1973): Isolation of a glucagon-containing peptide: Primary structure of a possible fragment of proglucagon. Proc. Nat. Acad. Sci. U. S. A. 70, 2321–2325.CrossRefGoogle Scholar
  10. 10.
    Unger, R. H., Ohneda, A., Valverde, I., Eisentraut, A. M. and Exton, J. (1968): Characterisation of the response to circulating glucagon-like immunoreactivity to intraduodenal and intravenous administration of glucose. J. Clin. Invest. 47, 48–65.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • J. M. Conlon
    • 1
    • 2
  • K. D. Buchanan
    • 1
    • 2
  • R. F. Murphy
    • 1
    • 2
  1. 1.Department of BiochemistryQueen’s University of BelfastBelfastNorthern Ireland
  2. 2.Department of MedicineQueen’s University of BelfastBelfastNorthern Ireland

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