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Culture Systems In Vitro for the Assay of Erythrocytic and Megakaryocytic Progenitors

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Abstract

The production of erythrocytic colonies from murine fetal liver cells seeded in semisolid plasma culture medium was first accomplished in 1971.1 The plasma culture system was then improved2 and is now widely used along with the methylcellulose system3 for the assay of the erythropoietic progenitors, CFU-e and BFU-e, of adult as well as fetal hemopoietic tissues of several species including man.

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References

  1. Stephenson JR, Axelrad AA, McLeod DL, et al: Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc Natl Acad Sci USA. 68: 1542–1546, 1971

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© 1978 Springer-Verlag New York Inc.

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McLeod, D.L., Shreeve, M.M., Axelrad, A.A. (1978). Culture Systems In Vitro for the Assay of Erythrocytic and Megakaryocytic Progenitors. In: Murphy, M.J., Peschle, C., Gordon, A.S., Mirand, E.A. (eds) In Vitro Aspects of Erythropoiesis. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6301-2_6

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  • DOI: https://doi.org/10.1007/978-1-4612-6301-2_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-6303-6

  • Online ISBN: 978-1-4612-6301-2

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