Preparation of Bleomycinic Acid from Bleomycin A2

  • Akio Fujii

Abstract

Twenty-two grams of bleomycin A2 (copper-containing hydrochloride powder) was heated at 100°C under diminished pressure (15 mm Hg) for 16 hr and then dissolved in 200 ml of 0.05 M ammonium chloride. The resulting solution was applied to an 8OQ-ml CM-Sephadex C-25 column pretreated with the same solvent. Then 690 ml of 0.1 M ammonium chloride was passed through the column, and the blue eluate was collected. The eluate was passed through a 500-ml column of activated charcoal, and the column was washed with water to remove inorganic salts. A 1:1 0.02 M HCI-acetone mixture was then passed through the column, and the blue eluate containing bleomycin demethyl-A2 (copper-containing hydrochloride) was collected. A basic anion-exchange resin, Dowex 44 (OH- form) was added to the collected fraction and the pH was adjusted to 6.0. After removing the solvent by distillation and drying, 8.91 g of a blue powder of bleomycin demethyl-A2 (copper-containing hydrochloride), mp 201-212°C (decomposion upon melting), was obtained.

Keywords

Acetone Hydroxide Bromide Pyridine Sodium Hydroxide 

References

  1. 1.
    H. Umezawa, T. Takita, A. Fujii, and H. Ito, U.S. Patent No.3, 960, 834 (1976).Google Scholar
  2. 2.
    H. Umezawa, T. Takita, A. Fujii, and T. Fukuoka, U.S. Patent No.3, 886, 133 (1975).Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1979

Authors and Affiliations

  • Akio Fujii

There are no affiliations available

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