Cell fusion normally occurs in vivo. The multinucleated muscle cells and osteoclasts are the products of cell fusion. Fusion of cells in culture was observed by Warren H. Lewis in the 1920s, however, utilization of the cell fusion phenomenon as a tool to study a number of biological problems started with Georges Barski and his associates (Barski et al., 1960). In his time-lapse motion pictures of cells in culture, Barski observed that two cells may fuse occasionally to form a single cell. To prove that fused cells can perpetuate themselves and contain two sets of genomes, Barski used as his test materials two mouse cell lines originally established by Katherine Sanford. These two clone lines were isolated from a mouse cell culture and became transplantable. They differed in their ability to induce tumors when injected into histocompatible mice and in chromosome characteristics. One line had a stemline chromosome number of 55 with no metacentric marker chromosomes, whereas the second line had a stemline number of 62 with from 9 to 19 biarmed markers.
KeywordsHuman Chromosome Thymidine Kinase Sendai Virus Hybrid Cell Line Chromosome Elimination
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