Cholesteryl Ester-Rich Very Low Density Lipoproteins: Magnetic Resonance Studies

  • Joel D. Morrisett
  • Richard K. Stockton
  • Roger D. Knapp
Conference paper

Abstract

A number of different animals have been used to model human atherosclerosis. Among these are the nonhuman primates, pigs, rabbits, guinea pigs, chickens, and rats. Of these animal models, the rabbit has been the most intensely- studied. Although the rabbit has a natural lesion distribution and lipid metabolism which is significantly different from that of man, this animal affords a number of unique advantages including low probability for spontaneous atherosclerosis, fast response to dietary or mechanical intervention, low cost, and facile handling. Furthermore, dietary refinements and careful drug administration have permitted the production of atherosclerotic lesions in the medium and large arteries of the rabbit which closely mimic those found in man. The types and locations of these induced lesions are now well-known. Over the past 50 years, the cholesterol-fed rabbit has been studied extensively as an experimental model for determining the relationship between dietary lipids and atherosclerosis. When the normal chow diet is enriched with 0.5-2.0% cholesterol, rabbits rapidly develop hyper- cholesterolemia (Duff 1935; Shore et al. 1974; Shumaker 1956) with subsequent appearance of atheromata (Anitschkow 1933; Kritchevsky 1954). While initially the cholesterol diet was utilized only to study induction of atherosclerotic lesions (Duff 1935), during the past 20 years a major focus of interest has been on the characterization of cholesterol-induced changes in the plasma lipoproteins.

Keywords

Cholesterol Ethyl Carboxyl Carbonyl Calorimetry 

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References

  1. Anitschko A (1933) Experimental arteriosclerosis in animals. In: Cowdry EV (ed) Arteriosclerosis. The Macmillan Company, New YorkGoogle Scholar
  2. Camejo G, Bosch V, Arreaza C, Mendez HC (1973) Early changes in plasma li-poprotein structure and biosynthesis in cholesterol-fed rabbits. J Lipid Res 14: 61–68PubMedGoogle Scholar
  3. Camejo G, Bosch V, Lope A (1974) The very low density lipoproteins of cholesterol-fed rabbits. Atherosclerosis 19: 139–152PubMedCrossRefGoogle Scholar
  4. Castellino FJ, Thomas JK, Ploplis VA (1977) Microviscosity of the lipid domains of normal and hypercholesterolemic very low density lipoproteins. Biochem Biophys Res Commun 75: 857–862PubMedCrossRefGoogle Scholar
  5. Duff GL (1935) Experimental cholesterol arteriosclerosis and its relationship to human arteriosclerosis. Arch Pathol 20: 82–123, 259-304Google Scholar
  6. Gofman JW, Lindgren F, Elliott H, Mantz W, Hewitt J, Strisower B, Herrin V (1950) The role of lipids and lipoproteins in atherosclerosis. Science 111: 166–171PubMedCrossRefGoogle Scholar
  7. Hamilton JA, Oppenheimer N, Cordes EH (1977) Carbon-13 nuclear magnetic resonance studies of choiesteryl esters and cholesteryl ester/triglyceride mixtures. J Biol Chem 252: 8071–8080PubMedGoogle Scholar
  8. Hubbell WL, McConnell HM (1971) Molecular motion in spin-labeled phospho-lipids and membranes. J Amer Chem Soc 93: 314–326CrossRefGoogle Scholar
  9. Jackson RL, Morrisett JD, Pownall HJ, Gotto, AM, Kamio A, Imai H, Tracy R, Kummerow FA (1977) Influence of dietary TRANS-fatty acids on swine li-poprotein composition and structure. J Lipid Res 18: 182–190PubMedGoogle Scholar
  10. Kritchevsky D, Moyer AW, Tesar WC, Logan JB, Brown RA, Davies MD, Cox HR (1954) Effect of cholesterol vehicle in experimental atherosclerosis. Am J Physiol 178: 30–32PubMedGoogle Scholar
  11. Morrisett JD, Pownall HJ, Jackson RL, Segura R, Gotto AM, Taunton OD (1977) Effects of polyunsaturated and saturated fat diets on the chemical composition and thermotropic properties of human plasma lipoproteins. In: Holman RT, Kunau W-H (eds) Polyunsaturated fatty acids, American Oil Chemists’ Society Monograph #4. AOCA Publishers, Champaign, Illinois, pp 139–161Google Scholar
  12. Morrisett JD, Pownall HJ, Roth RI, Gotto AM, Patsch JR (1979) Structure of rabbit cholesteryl ester-rich very low density lipoproteins (CER-VLDL). Biophys J 25: 286aGoogle Scholar
  13. Ploplis VA, Thomas JK, Castellino FJ (1979) Comparative studies of the physical state of the lipid phase of normal and hypercholesterolemic very low density lipoprotein. Chem Phys Lipids 23: 49–62PubMedCrossRefGoogle Scholar
  14. Sears B, Deckelbaum RJ, Janiak M, Shipley GG, Small DM (1976) Temperature-dependent 13C nuclear magnetic resonance studies of human serum low density lipoproteins. Biochemistry 15: 4151–4157PubMedCrossRefGoogle Scholar
  15. Seelig, J (1976) Anisotropic motion in liquid crystalline structures. In: Berliner LJ (ed) Spin labeling: theory and techniques. Academic Press, Inc., New York, Chapter 10Google Scholar
  16. Shore VG, Shore B, Hart RG (1974) Changes in apolipoproteins and properties of rabbit very low density lipoproteins on induction of cholesterolemia. Biochemistry 13: 1579–1585PubMedCrossRefGoogle Scholar
  17. Shumaker VN (1956) Cholesterolemic rabbit lipoproteins—serum lipoproteins of cholesterolemic rabbits. Am J Physiol 184: 35–42Google Scholar
  18. Small DM (1970) The physical state of lipids of biological importance: cholesteryl esters, cholesterol, triglyceride. Adv Exp Med Biol 26: 55–83Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Joel D. Morrisett
  • Richard K. Stockton
  • Roger D. Knapp

There are no affiliations available

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