Abstract
Cells of many animal species and of diverse tissue origins can be grown and maintained under defined and reproducible conditions in cell culture. During the past five years we have gained information and valuable insight from cell culture studies of lipid synthesis, lipoprotein uptake and degradation and factors which regulate the net cholesterol content of cells and tissues. Recent studies on the hepatic contribution to lipoprotein synthesis have utilized either freshly isolated rat hepatocytes (Jeejeebhoy and Phillips 197 6) or single-pass and recirculating rat liver perfusions (Marsh 1974, 1976; Hamilton et al. 1976). Primary monolayer cultures of adult rat hepatocytes which maintain hepatic biosynthetic functions for periods of several days, rather than hours, can provide advantages over the short-term isolated cell and organ perfusion systems.
This work was supported by NIH research grant HL-14197 awarded by the National Heart, Lung and Blood Institute and by NIH Postdoctoral Fellowship AM-0564 (to RAD).
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Weinstein, D.B., Davis, R.A., Engelhorn, S.C., Steinberg, D. (1980). Synthesis and Secretion of Very Low Density Lipoprotein by Cultured Rat Hepatocytes. In: Gotto, A.M., Smith, L.C., Allen, B. (eds) Atherosclerosis V. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6071-4_36
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DOI: https://doi.org/10.1007/978-1-4612-6071-4_36
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