Smooth Muscle Cell Kinetics in Vivo: A Comparative Study
The importance of the vascular smooth muscle cell (SMC) in atherogenesis has been well established (Wissler 1973; Ross and Glomset 1973). As a result, these cells have come under intense scrutiny for their synthetic and growth capacities; many of these studies have been carried out in tissue culture. Some studies have been performed in vivo, but have been hampered by the difficulty in obtaining quantitative data other than by performing autoradio- graphy or morphometry. These latter techniques are time-consuming and require large experimental populations to provide accurate measurements. We have recently developed a quantitative assay for SMC growth kinetics in vivo by measuring SMC DNA specific activity (Goldberg et al. 1979). This assay provides an accurate and rapid assessment of SMC growth following endothelial removal. We have applied this assay to both the rabbit and rat to determine differences in SMC growth kinetics among the different animals.
KeywordsCatheter Fractionation Thymidine Dehyde Cacodylate
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- Fowler S, Shio H, Wolinsk H (1977) Subcellular fractionation and morphology of calf aortic smooth muscle cells. Studies on whole aorta, aortic ex-plants and subculture grown under different conditions, J Cell Biol 75: 166–184Google Scholar
- Wissler RW (1973) Development of the atherosclerotic plaque. Hosp Prac 8: 3: 61–72Google Scholar