Smooth Muscle Cell Kinetics in Vivo: A Comparative Study

  • Michael B. Stemerman
  • Itzhak D. Goldberg
  • Ruth T. Gardner
  • Robert L. Fuhro
Conference paper

Abstract

The importance of the vascular smooth muscle cell (SMC) in atherogenesis has been well established (Wissler 1973; Ross and Glomset 1973). As a result, these cells have come under intense scrutiny for their synthetic and growth capacities; many of these studies have been carried out in tissue culture. Some studies have been performed in vivo, but have been hampered by the difficulty in obtaining quantitative data other than by performing autoradio- graphy or morphometry. These latter techniques are time-consuming and require large experimental populations to provide accurate measurements. We have recently developed a quantitative assay for SMC growth kinetics in vivo by measuring SMC DNA specific activity (Goldberg et al. 1979). This assay provides an accurate and rapid assessment of SMC growth following endothelial removal. We have applied this assay to both the rabbit and rat to determine differences in SMC growth kinetics among the different animals.

Keywords

Catheter Fractionation Thymidine Dehyde Cacodylate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baumgartner HR (1963) Eine neue methode zur erzeugung von thromben durch gezielte uberdehnung der gefasswand, Z Ges Exp Med 137: 227–236PubMedCrossRefGoogle Scholar
  2. Fowler S, Shio H, Wolinsk H (1977) Subcellular fractionation and morphology of calf aortic smooth muscle cells. Studies on whole aorta, aortic ex-plants and subculture grown under different conditions, J Cell Biol 75: 166–184Google Scholar
  3. Goldberg ID, Stemerman MB, Schnipper LE, Ransil BJ, Crooks GW, Fuhro RL (1979) Vascular smooth muscle cell kinetics: A new assay for studying patterns of cellular proliferation in vivo. Science 205: 920–922PubMedCrossRefGoogle Scholar
  4. Ross R, and Glomset JA (1973) Atherosclerosis and the arterial smooth muscle cell. Science 180: 1332–1339PubMedCrossRefGoogle Scholar
  5. Stemerman MB (1973) Thrombogenesis of the rabbit arterial plaque: An electron microscopic study. Am J Path 73: 7–26PubMedGoogle Scholar
  6. Stemerman MB, Spaet TH, Pitlick FA, Cintron S, Lejnieks I, Tiell ML (1977) Intimal healing: The pattern of re-endothelialization and intimai thickening. Am J Path 87: 125–137PubMedGoogle Scholar
  7. Wissler RW (1973) Development of the atherosclerotic plaque. Hosp Prac 8: 3: 61–72Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Michael B. Stemerman
  • Itzhak D. Goldberg
  • Ruth T. Gardner
  • Robert L. Fuhro

There are no affiliations available

Personalised recommendations