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Neurohormonal Techniques in Insects pp 116–136Cite as

Insulin-like and Glucagon-like Hormones in Insects

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Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

For many years, it has been known that the regulation of carbohydrate metabolism in vertebrates involves a multitude of hormones, enzymes, metabolites, and ions (Unger et al. 1977, 1978). Some of the hormones include insulin (the hypoglycemic hormone), glucagon (the hyperglycemic hormone), somatotropin and somatostatin (which regulate secretion of insulin and glucagon), adrenal glucocorticoids, and epinephine. The first two peptide hormones are the primary determinants necessary for adaptive carbohydrate changes in the vertebrate animal to respond to nutritional and environmental demands. Although the absolute concentration of these hormones is important, the concentration of one relative to the other and to other regulatory agents must also be considered by the target tissue. The target cell must respond to the ratio of effector concentrations, that is, to what may be termed the cellular effector charge (Atkinson 1977).

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References

  • Akanuma Y, Kuzuya T, Hayashi M, Ide T, Kuzuya N (1970) Immunological reactivity of insulin to sepharose coupled with insulin-antibody. Its use for the extraction of insulin from serum. Biochem Biophys Res Commun 38:947–953.

    Article  PubMed  CAS  Google Scholar 

  • Assan R (1973) Glucagon antisera. In: Berson SA, Yalow RS (eds) Methods in investigative and diagnostic endocrinology. North-Holland, Amsterdam.

    Google Scholar 

  • Atkinson DE (1977) Cellular energy metabolism and its regulation. Academic, New York.

    Google Scholar 

  • Baetens O, Rufener C, Srikant BC, Dobbs R, Unger R, Orci L (1976) Identification of glucagon-producing cells (A-cells) in dog gastric mucosa. J Cell Biol 69:455–464.

    Article  PubMed  CAS  Google Scholar 

  • Bell RA, Joachim FG (1976) Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. Ann Entomol Soc Am 69:365–373.

    Google Scholar 

  • Berson SA, Yalow RS (1961) Immunochemical distinction between insulin with identical amino acid sequences Nature 191:1392–1393.

    Article  PubMed  CAS  Google Scholar 

  • Berson SA, Yalow RS (1966) Insulin in blood and insulin antibodies. Am J Med 40:676–690.

    Article  PubMed  CAS  Google Scholar 

  • Berson SA, Yalow RS (1973) Peptide hormone radioimmunoassay. In: Berson SA, Yalow RS, (eds) Methods in investigative and diagnostic endocrinology. North-Holland, Amsterdam.

    Google Scholar 

  • Bhakthan NMG, Gilbert LI (1968) Effects of some vertebrate hormones on lipid solubilization in insect fat body. Gen Comp Endocrinol 11:186–197.

    Article  PubMed  CAS  Google Scholar 

  • Bowers WS, Friedman S, (1963) Mobilization of fat body glycogen by an extract of corpus cardacium. Nature 198:685.

    Article  CAS  Google Scholar 

  • Brown BE (1965) Pharmacologically active constituents of the cockroach corpus cardiacum: resolution and some characteristics. Gen Comp Endocrinol 5:387–401.

    Article  PubMed  CAS  Google Scholar 

  • Carroll NV, Longley RW, Roe JH (1956) The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem 220:583–593.

    PubMed  CAS  Google Scholar 

  • Chan S, Keim P, Steiner DF (1976) Cell free synthesis of rat preproinsulins: characterization and partial amino acid sequence determination Proc Natl Acad Sci USA 73:1964–1967.

    Article  PubMed  CAS  Google Scholar 

  • Chang F (1974) Effect of vertebrate adipokinetic hormones on the rate of in vitro lipid release in insects. Comp Biochem Physiol 49B:567–578.

    Google Scholar 

  • Chen AC, Friedman S (1977) Hormonal regulation of trehalose metabolism in the blowfly, Phormia regina; interaction between hypertrahalosemic and hypotrehalsemic hormones. J Insect Physiol 23:1223–2132.

    Article  PubMed  CAS  Google Scholar 

  • Collip JB (1973) The demonstration of an insulin-like substance in the tissues of the clam (Mya aremaria). J Biol Chem 55:39.

    Google Scholar 

  • Coons A, Leduc E, Connolly J (1955) Studies on antibody production. 1. A method for the histochemical demonstration of specific antibody and its application to the study of the hyperimmune rabbit. J Exp Med 102:49–63.

    Article  PubMed  CAS  Google Scholar 

  • Davidson JK, Falkmer S, Mehrotra BK, Wilson S (1971) Insulin assays and light microscopic studies of digestive organs in protosomian and deuterostomian species and in Coelenterates. Gen Comp Endocrinol 17:388–401.

    Article  PubMed  CAS  Google Scholar 

  • Davis KT, Stearns A (1977) In vitro growth of imaginai disks from Drosophila helanogaster. Science 196:438–440.

    Article  PubMed  CAS  Google Scholar 

  • Dayhoff MO (1972) Hormones, active peptides and toxins. In: Atlas of protein sequence and structure 5. National Biomedical Research Foundation, Silver Springs, Maryland.

    Google Scholar 

  • Dixit PK, Patel NG (1964) Insulin-like activity in larval foods of the honeybee Nature 202:189–190.

    Article  PubMed  CAS  Google Scholar 

  • Dutrieu J, Gourdoux L (1965) Le controle neuroendocrinen de 1a trehalosemic de Carausius morosus. Gen Comp Endocrinol 5:387–401.

    Article  Google Scholar 

  • Falkmer S (1972) Insulin production in vertebrates and invertebrates Gen Comp Endocrinol 3:184–191.

    Article  Google Scholar 

  • Falkmer S, Ostberg Y (1976) Production of islet hormones in invertebrates cyclostomes and primitive gnathostomes. In: Grillo TAJ, Leibson L, Epple A (eds) The evolution of pancreatic islets. Pergamon, Oxford.

    Google Scholar 

  • Falkmer S, Ostberg Y (1977) Comparative morphology of pancreatic islets in animals. In: Volk B, Wellmann K (eds) The diabetic pancreas. Plenum New York.

    Google Scholar 

  • Falkmer S, Wilson S (1967) Comparative aspects of the immunology and biology of insulin Diabetologia 3:519–528.

    Article  PubMed  CAS  Google Scholar 

  • Falkmer S, Emdin S, Havu N, Lundgren G, Margques M, Ostberg Y, Steiner DF, Thomas HW (1973) Insulin in invertebrates and cyclostomes. Am Zool 13:625–638.

    CAS  Google Scholar 

  • Falkmer S, Cutfield J, Cutfield S, Dodson G, Gliemann J, Gammeltoft S Marques M, Peterson JO, Steiner D, Sundby F, Emdin S, Havu N, Ostberg Y, Wmbladh L (1975) Comparative endocrinology of insulin and glucagon production. Am Zool 15(Suppl.):255–270.

    Google Scholar 

  • Friedman S (1967) The control of trehalose synthesis in the blowfly, Phormia regina Meig. J Insect Physiol 13:397–405.

    Article  PubMed  CAS  Google Scholar 

  • Fritsch H, Van Norden S, Pearse A (1976) Cytochemical and immunofluorescence investigations of insulin-like producing cells in the intestine of Mytilus edulis L. (Bivalvia). Cell Tissue Res 165:365–369.

    Article  PubMed  CAS  Google Scholar 

  • Goldsworthy GJ (1969) Hyperglycemic factors from the corpus cadiacum of Locusta migratoria. J Insect Physiol 15:2131–2140.

    Article  CAS  Google Scholar 

  • Helmstaedter V, Feurle G, Forsmenn W (1976) Immunocytochemical evidence for insulin-like and glucagon-like peptides. Cell Tissue Res 172:447–454.

    Article  PubMed  CAS  Google Scholar 

  • Hemmingsen AM (1934) The action of insulin in the frog and some invertebrates. Skard Arc Physiol 46:56–63.

    Google Scholar 

  • Humason GL (1972) Animal tissue techniques, 3rd edn. Freeman, San Francisco.

    Google Scholar 

  • Ishay J (1975) Glucose levels in Vespa orientalis: the effect of starvation. Comp Biochem Physiol 52A:91–96.

    Article  Google Scholar 

  • Ishay J, Glitten S, Galun R, Doron M, Leron Z (1976) The presence of insulin in and some effects of exogenous insulin on Hymenoptera tissues and body fluids. Comp Biochem Physiol 54A:203–206.

    Article  Google Scholar 

  • Keeley LI, Friedman S (1967) Corpus cardiacum as a metabolic regulator in Blaberas discoidalis. 1. Long-term effects of cardiatectony on whole body and tissue respiration and in trophic metabolism. Gen Comp Endocrinol 8:129–134.

    Article  CAS  Google Scholar 

  • Kimmel JR, Pollack HG, Hazelwood RL (1968) Isolation and characterization of chicken insulin. Endocrinology 83:1323–1330.

    Article  PubMed  CAS  Google Scholar 

  • Kirkham KE, Hunter WM (1971) Radioimmunoassay methods. Livingstone, London.

    Google Scholar 

  • Kramer KJ, Tager HS, Childs CN, Speirs RD (1977) Insulin-like hypoglycemic and immunological activities in honeybee royal jelly. J Insect Physiol 23:293–295.

    Article  PubMed  CAS  Google Scholar 

  • Kramer KJ, Speirs RD, Childs CN (1978) A method for separation of trehalose from insect hemolymph. Anal Biochem 86:692–696.

    Article  PubMed  CAS  Google Scholar 

  • Maier V, Witznick G, Keller R, Pfeiffer EF (1978) Insulin-like and glucagon-like immunoreactivities in the honeybee (Apis mellifera). Acta Endocrinol 87:69–70.

    Google Scholar 

  • deMartinez NR, Garcia MC, Salas M, Candela JLR (1973) Proteins with insulin like activity isolated from oyster (Ostrea edulls L.) hepatopancreas. Gen Comp Endocrinol 20:305–311.

    Article  PubMed  Google Scholar 

  • McLaughlin PJ, Dayhoff MO (1972) Evolution of species and proteins: a time scale. In: Dayhoff M (ed) Atlas of protein sequence and structure. National Biomedical Research Foundation, Silver Springs, Maryland.

    Google Scholar 

  • Meneses P, Ortiz M (1975) A protein extract from Drosophilia melanogaster with insulin-like activity. Comp Biochem Physiol A51:483–485.

    Article  Google Scholar 

  • Mosna G, Barigozzi C (1976) Stimulation of growth by insulin in Drosophila embryonic cells in vitro. Experientia 32:855–856.

    Article  PubMed  CAS  Google Scholar 

  • Natalizi GM, Frontali N (1966) Purification of insect hyperglycemic and heart accelerating hormones. J Insect Physiol 12:1279–1287.

    Article  Google Scholar 

  • Natalizi GM, Pansa MC, D’Ajello V, Casaglia O, Bettini S, Frontali N (1970) Physiologically active factors from corpora cardiaca of Periplaneta ameri-cana. J Insect Physiol 16:1827–1836.

    Article  Google Scholar 

  • Normann TC (1975) Neurosecretory cells in insect brain and production of hypoglycemic hormone. Nature 254:259–261.

    Article  PubMed  CAS  Google Scholar 

  • Normann TC, Duve H (1969) Experimentally induced release of a neurohormone influencing hemolymph trehalose level in Calliphora erythrocephala. Gen Comp Endocrinol 12:449–459.

    Article  PubMed  CAS  Google Scholar 

  • Normann TC, Duve H (1978) Insulin-like and glucagon-like neurohormones in the blowfly. In: Gaillard PJ, Boer HH (eds) Proc VIII Int Symp Comp Endocrinol. Elsevier, Amsterdam.

    Google Scholar 

  • Orci L, Baetens D, Ravazzola M, Stefan Y, Malaisse-Lagal F (1976) Pancreatic polypeptide and glucagon non-random distribution in pancreatic islets. Life Sci 19:1811–1816.

    Article  PubMed  CAS  Google Scholar 

  • Pearse A (1969) The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J Histochem Cytochem 17:303–313.

    Article  PubMed  CAS  Google Scholar 

  • Plisetskaya E, Kazakov V, Soltitskaya L, Leibson L (1978) Insulin producing cells in the gut of freshwater bivalve molluscs and the role of insulin in the regulation of their carbohydrate metabolism. Gen Comp Endocrinol 35:133–145.

    Article  PubMed  CAS  Google Scholar 

  • Roe JH (1955) The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem 212:335–343.

    PubMed  CAS  Google Scholar 

  • Sasaki H, Rubalcava B, Baetens O, Blaunez E, Stikant C, Orci L, Unger R (1975) Identification of glucagon in the gastrointestinal tract. J Clin Invest 56:135–145.

    Article  PubMed  CAS  Google Scholar 

  • Schneiderman HA (1967) Insect surgery. In: Wessels WH (ed) Methods in developmental Biology. Cromwell, New York.

    Google Scholar 

  • Seecof RC, Dewhurst S (1974) Insulin is a Drosophila hormone and acts to enhance the diiferientation of embryonic Drosophilia cells. Cell Diff 3:63–70.

    Article  CAS  Google Scholar 

  • Smith LF (1966) Species variation in the amino acid sequence of insulin. Am J Med 40:662–666.

    Article  PubMed  CAS  Google Scholar 

  • Steele JE (1961) Occurrence of a hyperglycemic factor in the corpus cardiacum of an insect. Nature 192:680–681.

    Article  Google Scholar 

  • Steele JE (1976) Hormonal control of metabolism in insects. Adv Insect Physiol 12:239–323.

    Article  CAS  Google Scholar 

  • Steiner DF (1976) Biosynthesis of peptide hormones. In: Parsons JA (ed) Peptide hormones. MacMillan, New York.

    Google Scholar 

  • Sternberger LA (1977) Immunocytochemistry of neuropeptides and their receptors. In: Gaines H (ed) Peptides in neurobiology. Plenum, New York.

    Google Scholar 

  • Sundler F, Alumets J, Holst J, Larsson L, Hakanson R (1976) Ultrastructural identification of cells storing pancreatic-type glucagon in dog stomach. Histochemistry 50:33–37.

    Article  PubMed  CAS  Google Scholar 

  • Tager HS, Steiner DF (1973) Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon. Proc Natl Acad Sci USA 70:2321–2325.

    Article  PubMed  CAS  Google Scholar 

  • Tager HS, Rubenstein AH, Steiner DF (1973) Methods for the assessment of peptide precursors. Studies on insulin biosynthesis. Methods Enzymol 37:326–345.

    Article  Google Scholar 

  • Tager HS, Markese J, Speirs RD, Kramer KJ (1975) Glucagon-like immunoreac-tivity in insect corpus cardiacum. Nature 254:707–708.

    Article  PubMed  CAS  Google Scholar 

  • Tager HS, Markese J, Kramer KJ, Speirs RD, Childs CN (1976) Glucagon-like and insulin-like hormones of the insect neurosecretory system. Biochem J 156:515–520.

    PubMed  CAS  Google Scholar 

  • Unger R, Ipp E, Schusdziarra V, Orci L (1977) Hypothesis: physiologic note of pancreatic somatostation and the contribution of D-cell disorders to diabetes mellitus. Life Sci 20: 2081–2085.

    Article  PubMed  CAS  Google Scholar 

  • Unger R, Dobbs R, Orci L (1978) Insulin, glucagon, and somatstatin secretion in the regulation of metabolism. Ann Rev Physiol 40:307–343.

    Article  CAS  Google Scholar 

  • Wiens AW, Gilbert LI (1967) Regulation of carbohydrate mobilization and utilization in Leucophaea maderae. J Insect Physiol 13:779–794.

    Article  PubMed  CAS  Google Scholar 

  • Wenig K, Joachim J (1936) Der Einfluss des Insulins auf den lymphzuckerspiegel bei der seidenaupe (Bombyx mori) Biochem Z 285:98–100.

    CAS  Google Scholar 

  • Wilson S, Falkmer S (1965) Starfish insulin. Can J Biochem 43:1615–1624.

    Article  PubMed  CAS  Google Scholar 

  • Yalow RS (1976) Methods in radioimmunoassay of peptide hormones. North Holland, Amsterdam.

    Google Scholar 

  • Yalow RS, Berson SA (1964) Reaction of fish insulins with human insulin antiserums. New Engl J Med 270:1171–1781.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Karl J. Kramer
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Editors and Affiliations

  1. Department of Entomology, University of California, Riverside, California, 92521, USA

    Thomas A. Miller

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© 1980 Springer-Verlag New York Inc

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Kramer, K.J. (1980). Insulin-like and Glucagon-like Hormones in Insects. In: Miller, T.A. (eds) Neurohormonal Techniques in Insects. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6039-4_5

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  • DOI: https://doi.org/10.1007/978-1-4612-6039-4_5

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-6041-7

  • Online ISBN: 978-1-4612-6039-4

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