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Maturation of the Lens Fiber Cell: Some Morphological and Biochemical Correlates

  • H. Maisel
  • J. Alcala
  • J. Kuszak
  • M. Ireland
  • R. Bradley
  • M. Katar
Part of the Ocular Size and Shape book series (EYE)

Abstract

The function of the lens is to refract light for proper visual acuity. As an organ it is characterized by several unique features. It is derived solely from ectodermal cells, and retains within it all the cells formed during the lifetime of the organism. It continues to grow throughout life, adding new fibers to those previously formed. Thus its structure contains, in concentric layers, fiber cells of increasing age proceeding from superficial (cortex) to deep (nucleus) fiber groups. During lens fiber cell differentiation there is a loss of cell organelles and the more centrally placed fibers appear to be metabolically inert (Wannemacher and Spector, 1968). The lens derives its nutrients from the aqueous humour.

Keywords

Fiber Cell Human Lens Lens Fiber Lens Fiber Cell Bovine Lens 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alcalá, J., N. Lieska and H. Maisel. 1975. Protein composition of bovine lens cortical fiber cell membranes. Exp. Eye Res. 21; 585–595.CrossRefGoogle Scholar
  2. Alcalá, J., H. Maisel and N. Lieska. 1977. The presence of delta crystallin in the plasma membrane of chick lens fiber cells. Exp. Cell Res. 109; 63–69.PubMedCrossRefGoogle Scholar
  3. Alcalá, J. and H. Maisel. 1978. Specific antiserum to the main intrinsic membrane polypeptide of chick lens fiber cell plasma membrane. Exp. Eye Res. 26; 219–221.PubMedCrossRefGoogle Scholar
  4. Alcalá, J., J. Kuszak, M. Katar, R. H. Bradley and H. Maisel. 1979. Relationship of intrinsic and peripheral proteins to chick lens gap junction morphology. J. Cell Biol. 83; 269(a).Google Scholar
  5. Alcalá, J., J. Valentine and H. Maisel. 1980. Human lens plasma membranes. I. Isolation, polypeptide composition and changes associated with ageing. Exp. Eye Res. 30; 659–677.PubMedCrossRefGoogle Scholar
  6. Bagchi, M., P. A. Gordon, J. Alcala and H. Maisel. 1979. The plasma membrane of the rabbit lens cortical fiber. Invest. Ophthal. 18; 562–569.Google Scholar
  7. Benedetti, E. L., I. Dunia and H. Bloemendal. 1974. Development of gap junctions during differentiation of lens fibers. Proc. Nat’l Acad. Sci. (USA) 71; 5073–5077.CrossRefGoogle Scholar
  8. Bloemendal, H. 1977. The vertebrate eye lens. Science 197; 127–138.PubMedCrossRefGoogle Scholar
  9. Bloemendal, H., E. L. Benedetti, F. S. Ramaekers, I. Dunia, M. A. Kibbelaar and A. J. M. Vermorken. 1979. Is the cytoskeleton-plasma membrane complex involved in lens protein biosynthesis. Molec. Biol. Rep. 5; 99–103.CrossRefGoogle Scholar
  10. Bradley, R. H., M. Ireland and H. Maisel. 1979. The cytoskeleton of chick lens cells. Exp. Eye Res. 28; 441–453.PubMedCrossRefGoogle Scholar
  11. Bradley, R. H., W.-K. Lo, J. Kuszak and H. Maisel. The cytoskeleton of the chicken lens: A scanning and ultrastructural analysis. Submitted.Google Scholar
  12. Broekhuyse, R. M. and E. D. Kuhlmann. 1974. Lens membranes. I. Composition of urea-treated plasma membranes from calf lens. Exp. Eye Res. 19; 297–302.PubMedCrossRefGoogle Scholar
  13. Broekhuyse, R. M., E. D. Kuhlmann and A. L. H. Stols. 1976. Lens membranes.Google Scholar
  14. II. Isolation and characterization of the main intrinsic membrane polypeptide of bovine lens fiber cells. Exp. Eye Res. 23; 365-371.Google Scholar
  15. Broekhuyse, R. M. and E. D. Kuhlmann. 1978. Lens membranes. IV. Preparative isolation and characterization of membranes and various membrane proteins from calf lens. Exp. Eye Res. 26; 305–320.PubMedCrossRefGoogle Scholar
  16. Broekhuyse, R. M., E. D. Kuhlmann and H. J. Winkens. 1979. Lens membranes. VII. MIP is an immunologically specific component of lens fiber membranes and is identical with 26K band protein. Exp. Eye Res. 29; 303–313.PubMedCrossRefGoogle Scholar
  17. Dickson, D. H. and G. W. Crock. 1972. Interlocking patterns of primate lens fibers. Invest. Ophthal. 13; 809–815.Google Scholar
  18. Dische, Z. 1965. The glycoproteins and glycolipoproteins of the bovine lens and their relation to albuminoid. Invest. Ophthal. 4; 759–778.PubMedGoogle Scholar
  19. Duke-Elder, S. and D. Abrams. 1970. The refraction of light. In: Systems of Ophthalmology, Vol. 5. S. Duke-Elder, ed. C. V. Mosby Co., St. Louis, pp. 74–77.Google Scholar
  20. Duncan, G. 1974. Comparative physiology of the lens membranes. In: The Eye, Vol. 5. H. Dawson and L. T. Graham, eds. Academic Press, New York. pp. 357–398.Google Scholar
  21. Farnsworth, P. N., S. C. J. Fu, P. A. Burke and I. Bahia. 1974. Ultra-structure of rat eye lens fibers. Invest. Ophthal. 13; 274–279.PubMedGoogle Scholar
  22. Goodenough, D. A. 1979. Lens gap junctions: A structural hypothesis for non-regulated low resistance intercellular pathways. Invest. Ophthal. 18; 1104–1122.Google Scholar
  23. Hamburger, V. and H. L. Hamilton. 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88; 49–92.CrossRefGoogle Scholar
  24. Harding, C. V., J. R. Reddan, N. J. Unakar and M. Bagchi. 1971. The control of cell division in the ocular lens. Int. Rev. Cytology 31; 215–300.CrossRefGoogle Scholar
  25. Harding, V., S. Susan and H. Murphy. 1976. Scanning electron microscopy of the adult rabbit lens. Ophthal. Res. 8; 443–455.CrossRefGoogle Scholar
  26. Harding, J. J. and K. J. Dilley. 1976. Structural proteins of the mammalian lens. A review with emphasis on changes in development, ageing, and cataract. Exp. Eye Res. 22; 1–74.PubMedCrossRefGoogle Scholar
  27. Horwitz, J., N. P. Robertson, M. M. Wong, J. S. Zigler and J. H. Kinoshita. 1979. Some properties of lens plasma membrane polypeptides isolated from normal human lenses. Exp. Eye Res. 28; 359–365.PubMedCrossRefGoogle Scholar
  28. Horwitz, J. and M. M. Wong. 1980. Peptide mapping by limited proteolysis in sodium dodecyl sulfate of the main intrinsic membrane polypeptides isolated from human and bovine lens plasma membranes. Biochim. Biophys. Acta 622; 134–143.PubMedGoogle Scholar
  29. Ikeda, A. and H. Maisel. 1975. An immuno-electron microscopic study of lens proteins. Ophthal. Res. 7; 204–208.CrossRefGoogle Scholar
  30. Ireland, M., H. Maisel and R. H. Bradley. 1978. The rabbit lens cytoskeleton: An ultrastructural analysis. Ophthal. Res. 10; 231–236.CrossRefGoogle Scholar
  31. Jurand, A. and T. Yamada. 1967. Elimination of mitochondria during Wolffian lens regeneration. Ecp. Cell Res. 46; 636–638.CrossRefGoogle Scholar
  32. Kibbelaar, M. A. and H. Bloemendal. 1979. Fractionation of the water-insoluble proteins from calf lens. Exp. Eye Res. 29; 679–688.PubMedCrossRefGoogle Scholar
  33. Kibbelaar, Mac A., A-M. E. Selten-Versteegen, I. Dunia and E. L. Benedetti. 1979. Actin in mammalian lens. Eur. J. Biochem. 95; 543–549.PubMedCrossRefGoogle Scholar
  34. Kuszak, J., H. Maisel and C. V. Harding. 1978. Gap junctions of chick lens fiber cells. Exp. Eye Res. 27; 495–498.PubMedCrossRefGoogle Scholar
  35. Kuszak, J., J. Alcalá and H. Maisel. 1980a. Formation of gap junctions in lens development. Submitted.Google Scholar
  36. Kuszak, J., J. Alcalá and H. Maisel. 1980b. The surface morphology of embryonic and adult chick lens fiber cells. In Press.Google Scholar
  37. Kuszak, J., J. Alcalá and H. Maisel. 1980c. Biochemical and structural features of chick lens gap junctions. Exp. Eye Res. In Press.Google Scholar
  38. Kuwabara, T. 1975. The maturation of the lens cell: a morphological study. Exp. Eye Res. 20; 427–443.PubMedCrossRefGoogle Scholar
  39. Lasser, A. and E. A. Balazs. 1972. Biochemical and fine structure studies on the water-insoluble components of the calf lens. Exp. Eye Res. 13; 297–308.CrossRefGoogle Scholar
  40. Maisel, H. and M. M. Perry. 1972. Electron microscopic observation on some structural proteins of the chick lens. Exp. Eye Res. 14; 7–12.PubMedCrossRefGoogle Scholar
  41. Maisel, H., J. Alcala and N. Lieska. 1976. The protein structure of the chick lens fiber cell membrane and intracellular matrix. Doc. Ophthal. Proc. 8; 121–133.Google Scholar
  42. Maisel, H., N. Lieska and R. H. Bradley. 1978. Isolation of filaments of the chick lens. Experientia 34; 352–353.PubMedCrossRefGoogle Scholar
  43. Maisel, H. and N. Rasmussen. 1978. Water-insoluble proteins of the chick lens. Ophthal. Res. 10; 241–249.CrossRefGoogle Scholar
  44. Maisel, H. and J. Alcala. 1980. The plasma membrane of the normal lens and cataract. In: The Red Blood Cell and the Lens Metabolism. S. Srivastava, ed. Elsevier, Holland; 213–228.Google Scholar
  45. Mousa, G. Y. and J. R. Trevithick. 1977. Differentiation of rat lens epithelial cells in tissue culture. Dev. Biol. 60; 14–25.PubMedCrossRefGoogle Scholar
  46. Nasser, S., R. Bradley, J. Alcalá and H. Maisel. 1980. Regional differences in the composition of the bovine lens urea-soluble protein. Exp. Eye Res. 30; 109–113.PubMedCrossRefGoogle Scholar
  47. Palva, M. and A. Palkama. 1976. Electron-microscopical, histochemical and biochemical findings on the Na-K-ATPase activity in the epithelium of the rat lens. Exp. Eye Res. 22; 229–236.PubMedCrossRefGoogle Scholar
  48. Porte, A., A. Brini and M. E. Stoeckel. 1975. Fine structure of the lens epithelium. Am. J. Ophthal. 7; 623–634.Google Scholar
  49. Rae, J. L. 1973. The potential difference of the frog lens. Exp. Eye Res. 15; 485–494.PubMedCrossRefGoogle Scholar
  50. Rae, J. L. and T. Stacey. 1979. Lanthanum and procion yellow as extracellular markers in the crystalline lens of the eye. Exp. Eye Res. 28; 1–21.PubMedCrossRefGoogle Scholar
  51. Rafferty, N. S. and E. A. Esson. 1974. An electron microscopic study of adult mouse lens. Some ultrastructural specializations. J. Ultrastruc. Res. 46; 239–253.CrossRefGoogle Scholar
  52. Rafferty, N. S. and W. Goossens. 1978. Cytoplasmic filaments in the crystalline lens of various species: Functional correlates. Exp. Eye Res. 26; 177–190.PubMedCrossRefGoogle Scholar
  53. Ramaekers, F. S., A.-M. E. Selten-Versteegen and H. Bloemendal. 1980. Interaction of newly synthesized a crystallin with isolated lens plasma membranes. Biochim. Biophys. Acta 596; 57–63.PubMedCrossRefGoogle Scholar
  54. Ramaekers, F. C. S., A.-M. E. Selten-Versteegen, E. L. Benedetti, I. Dunia and H. Bloemendal. 1980. In vitro synthesis of the major lens membrane protein. Proc. Nat’l Acad. Sci. (USA) 77; 725–729.CrossRefGoogle Scholar
  55. Roy, D. 1979. Age-dependent changes in the abundance of the major polypeptides of human lens membrane. Biochem. Biophys. Res. Comm. 88; 30–36.PubMedCrossRefGoogle Scholar
  56. Roy, D., A. Spector and P. N. Farnsworth. 1979. Human lens membrane: comparison of major intrinsic polypeptides from young and old lenses isolated by a new methodology. Exp. Eye Res. 28; 353–358.PubMedCrossRefGoogle Scholar
  57. Spector, A., M. H. Garner, W. H. Garner, D. Roy, P. Farnsworth and S. Shyne. 1979. An extrinsic membrane polypeptide associated with high molecular weight protein aggregates in human cataract. Science 204; 1323–1326.PubMedCrossRefGoogle Scholar
  58. Takemoto, L. J., J. S. Hansen and K. Horwitz. 1980. Interspecies conservation of the main intrinsic protein (MIP) of the lens membrane. Invest. Ophthal. Supp. April, pp. 86–87.Google Scholar
  59. Waggoner, P. T. and H. Maisel. 1978. Immunofluorescent study of a chick lens fiber cell membrane polypeptide. Exp. Eye Res. 27; 151–157.PubMedCrossRefGoogle Scholar
  60. Wannemacher, C. F. and A. Spector. 1968. Protein synthesis in the core of calf lens. Exp. Eye Res. 7; 623–625.PubMedCrossRefGoogle Scholar
  61. Wong, M. M., N. P. Robertson and J. Horwitz. 1978. Heat induced aggregation of the SDS-solubilized main intrinsic polypeptide isolated from bovine lens plasma membrane. Biochem. Biophys. Res. Comm. 84; 158–165.PubMedCrossRefGoogle Scholar
  62. Yorio, T. and R. J. Bentley. 1976. Distribution of the extracellular space of the amphibian lens. Exp. Eye Res. 23; 601–608.PubMedCrossRefGoogle Scholar
  63. Zelenka, P., R. Reszelbach and J. Piatigorsky. 1979. Developmental changes in proteins of purified membranes of chicken lenses and evidence for contamination by cytoplasmic delta crystalline. Biochem. et Biophys. Acta 556; 447–456.CrossRefGoogle Scholar
  64. Zigler, J. S., J. Horwitz and J. H. Kinoshita. 1980. Immunochemical studies on the major intrinsic proteins of lens membranes. Invest. Ophthal. Suppl. April, p. 87.Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1981

Authors and Affiliations

  • H. Maisel
  • J. Alcala
  • J. Kuszak
  • M. Ireland
  • R. Bradley
  • M. Katar

There are no affiliations available

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