Constructing and Analyzing Recombinant DNA
This chapter describes a number of procedures commonly used to construct, select, and characterize recombinant DNA. They could, for example, be used to obtain a recombinant DNA clone that contains a particular gene. The procedures are listed in approximately the same order as for a gene cloning project. The exception to this order is that RNA purification methods are grouped at the end of the chapter. Ordering the procedures according to a gene cloning scheme is convenient but somewhat misleading, since the procedures described are useful for many other purposes.
KeywordsCreatine Phosphokinase Microfuge Tube Fusaric Acid Hybridization Reaction Nitrocellulose Paper
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