Constructing and Analyzing Recombinant DNA

  • Robert F. Schleif
  • Pieter C. Wensink

Abstract

This chapter describes a number of procedures commonly used to construct, select, and characterize recombinant DNA. They could, for example, be used to obtain a recombinant DNA clone that contains a particular gene. The procedures are listed in approximately the same order as for a gene cloning project. The exception to this order is that RNA purification methods are grouped at the end of the chapter. Ordering the procedures according to a gene cloning scheme is convenient but somewhat misleading, since the procedures described are useful for many other purposes.

Keywords

Agar Agarose Chloroform Heparin Polypropylene 

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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • Robert F. Schleif
    • 1
  • Pieter C. Wensink
    • 2
  1. 1.Department of BiochemistryBrandeis UniversityWalthamUSA
  2. 2.Rosenstiel Center and Department of BiochemistryBrandeis UniversityWalthamUSA

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