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Constructing and Analyzing Recombinant DNA

  • Robert F. Schleif
  • Pieter C. Wensink

Abstract

This chapter describes a number of procedures commonly used to construct, select, and characterize recombinant DNA. They could, for example, be used to obtain a recombinant DNA clone that contains a particular gene. The procedures are listed in approximately the same order as for a gene cloning project. The exception to this order is that RNA purification methods are grouped at the end of the chapter. Ordering the procedures according to a gene cloning scheme is convenient but somewhat misleading, since the procedures described are useful for many other purposes.

Keywords

Creatine Phosphokinase Microfuge Tube Fusaric Acid Hybridization Reaction Nitrocellulose Paper 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • Robert F. Schleif
    • 1
  • Pieter C. Wensink
    • 2
  1. 1.Department of BiochemistryBrandeis UniversityWalthamUSA
  2. 2.Rosenstiel Center and Department of BiochemistryBrandeis UniversityWalthamUSA

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