Multi-well Assay for Unscheduled DNA Synthesis Using Human Diploid Fibroblasts
There is concern that man is being exposed to substances that have the potential to cause genetic disease. The development of a number of short-term tests for mutagenic activity (Kilbey et al., 1977; Montesano et al., 1976) has greatly facilitated the task of identifying mutagenic substances, and since it has been demonstrated that many mutagens are also carcinogens (McCannet al., 1975), these substances may also be carcinogenic. Short-term tests for mutagenic activity can be divided into two groups: (1) tests that result in specific types of mutation events (i.e., point mutation or chromosome aberration) that are directly detected as phenotypic changes; and (2) tests that detect effects that correlate with genetic events. These latter effects may be precursor events which could ultimately, but not necessarily, lead to the formation of mutations. This category includes tests for induction of DNA damage and stimulation of unscheduled DNA synthesis (Stich et al., 1976). Since they do not require the detection of a specific genotypic change which may require a rigid set of conditions, these indicator tests may be considered more universal in their ability to detect genetic activity. Thus they serve as an important adjunct to tests which measure mutations directly.
KeywordsDMSO Arginine NADPH Pyrene Adenine
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