Induction of a Resistant Preneoplastic Liver Cell as a New Principle for a Short-Term Assay in vivo for Carcinogens

  • Emmanuel Farber
  • Hiroyuki Tsuda
Conference paper
Part of the Topics in Environmental Physiology and Medicine book series (TEPHY)


The past few years have seen the development of a large number (over 60) of new tests designed to detect and identify possible carcinogens in man’s environment. The test organisms and the endpoints used vary widely in detail but in general the assays fall into two groups. The tests in the smallest group use a complex cellular response, cell transformation, as the indicator. This response resembles in several respects malignant neoplasia and is considered to be a valid in vitro analogue of cancer induction in vivo. The tests in the largest group use some aspect of DNA damage and/or repair or some presumed reflection of damage or repair of DNA, such as mutations, chromosome damage, chromatid exchanges, etc. The chemical-biochemical basis of the transformation tests is unknown.


Partial Hepatectomy Cancer Induction Liver Carcinogenesis Ethyl Carbamate Genotoxic Assay 
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  1. Bridges, B.A. (1976): Short-term screening tests for carcinogens. Nature (Lond.) 261: 195–200.CrossRefGoogle Scholar
  2. Cayama, E., Tsuda, H., Sarma, D.S.R., Farber, E. (1978): Initiation of chemical carcinogenesis requires cell proliferation. Nature (Lond.) 275: 60–62.CrossRefGoogle Scholar
  3. Farber, E. (1973): Carcinogenesis—cellular evolution as a unifying thread: Presidential address. Cancer Res. 33: 2537–2550.PubMedGoogle Scholar
  4. Farber, E. (1980): The sequential analysis of liver cancer induction. Biochim. Biophys. Acta 605: 149–166.PubMedGoogle Scholar
  5. Farber, E., Cameron, R. (1980): The sequential analysis of cancer development. Adv. Cancer Res. 57: 125–226.CrossRefGoogle Scholar
  6. Foulds, L. (1975): Neoplastic Development, Vol. 2. London: Academic Press.Google Scholar
  7. Ogawa, H., Medline, A., Farber, E. (1979): Sequential analysis of hepatic carcinogenesis: A comparative study of the ultrastructure of preneoplastic, malignant, prenatal, postnatal and regenerating liver. Lab. Invest. 40: 22–35.Google Scholar
  8. Ogawa, K., Solt, D., Farber, E. (1980): Phenotypic diversity as an early property of putative preneoplastic hepatocyte populations in liver carcinogenesis. Cancer Res. 40: 725–130.PubMedGoogle Scholar
  9. Rutenberg, A.M., Kim, H., Fischbein, J.W., Hanker, J.S., Wasserkrug, H.L., Seligman, A.M. (1969): Histochemical and ultrastructural demonstration of γ-glutamyl transpep-tidase activity. J. Histochem. Cytochem. 17: 517–526.CrossRefGoogle Scholar
  10. Solt, D., Farber, E. (1976): New principle for the analysis of chemical carcinogenesis. Nature (Lond.) 265: 701–703.CrossRefGoogle Scholar
  11. Solt, D., Farber, E. (1977): Persistence of carcinogen-induced initiated hepatocytes in liver carcinogenesis. Proc. Am Assoc. Cancer Res. 18: 52.Google Scholar
  12. Solt, D., Medline, A., Farber, E. (1977): Rapid emergence of carcinogen-induced hyperplastic lesions in a new model for the sequential analysis of liver carcinogenesis. Am. J. Pathol. 88: 595–618.PubMedGoogle Scholar
  13. Tsuda, H., Farber, E. (1979): Initiation of putative preneoplastic liver lesions by single doses of non-liver and liver carcinogens plus partial hepatectomy (PH). Proc. Am. Assoc. Cancer Res. 20: 15.Google Scholar
  14. Ying, T.S., Sarma, D.S.R. (1979): Role of cell necrosis in the induction of preneoplastic lesions. Proc. Am. Assoc. Cancer Res. 20: 14.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • Emmanuel Farber
  • Hiroyuki Tsuda

There are no affiliations available

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