The Use of H.P.L.C. for Structural Studies on Collagen

  • M. van der Rest
  • P. P. Fietzek
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)

Abstract

The establishment of collagen primary structure poses specific challenging problems that are linked to the size of collagen polypeptide chains, their unique amino acid compositions and repetitive sequences. The purification of the fragments needed for sequencing is particularly difficult with standard chromatographic techniques. We have thus developed a comprehensive H.P.L.C. approach for the purification of collagen chains and their CNBr and other proteolytic fragments.

Keywords

Acetonitrile Polypeptide Resi Chromato HFBA 

Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • M. van der Rest
    • 1
  • P. P. Fietzek
    • 2
  1. 1.Genetics UnitShriners HospitalMontrealCanada
  2. 2.Department of BiochemistryCMDNJ-Rutgers Medical SchoolPiscatawayUSA

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