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Correlation Between DNA and Protein Sequences in β-Galactosidase Related Proteins

  • Audree V. Fowler
  • Irving Zabin
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)

Abstract

The lactose operon of Escherichia coli has been a valuable tool for studying protein-nucleic acid interactions and correlations. Work in this laboratory is concerned not only with gene protein correlations, but also with structure-function relationships and evolution of proteins. The emphasis has been on lac proteins, in particular β-galactosidase. In order to understand and to define this system, it was important to determine the protein and nucleic acid sequences of the complete operon. The four proteins coded for by lacI, lacZ, lacY and lacA are the repressor (39,000 dalton subunit), β-galactosidase (116K), lac permease (46K) and thiogalactosidase transacetylase (25K), respectively (Fig. 1).

Keywords

Maltose Binding Protein Hybrid Protein Cyanogen Bromide Fusion Strain Amino Terminal Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • Audree V. Fowler
    • 1
  • Irving Zabin
    • 1
  1. 1.Department of Biological Chemistry, UCLA School of Medicine and Molecular Biology InstituteUniversity of CaliforniaLos AngelesUSA

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