Correlation Between DNA and Protein Sequences in β-Galactosidase Related Proteins
The lactose operon of Escherichia coli has been a valuable tool for studying protein-nucleic acid interactions and correlations. Work in this laboratory is concerned not only with gene protein correlations, but also with structure-function relationships and evolution of proteins. The emphasis has been on lac proteins, in particular β-galactosidase. In order to understand and to define this system, it was important to determine the protein and nucleic acid sequences of the complete operon. The four proteins coded for by lacI, lacZ, lacY and lacA are the repressor (39,000 dalton subunit), β-galactosidase (116K), lac permease (46K) and thiogalactosidase transacetylase (25K), respectively (Fig. 1).
KeywordsSugar Filtration Codon Bromide Carboxyl
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