Correlation Between DNA and Protein Sequences in β-Galactosidase Related Proteins

  • Audree V. Fowler
  • Irving Zabin
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)


The lactose operon of Escherichia coli has been a valuable tool for studying protein-nucleic acid interactions and correlations. Work in this laboratory is concerned not only with gene protein correlations, but also with structure-function relationships and evolution of proteins. The emphasis has been on lac proteins, in particular β-galactosidase. In order to understand and to define this system, it was important to determine the protein and nucleic acid sequences of the complete operon. The four proteins coded for by lacI, lacZ, lacY and lacA are the repressor (39,000 dalton subunit), β-galactosidase (116K), lac permease (46K) and thiogalactosidase transacetylase (25K), respectively (Fig. 1).


Maltose Binding Protein Hybrid Protein Cyanogen Bromide Fusion Strain Amino Terminal Region 
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  1. 1.)
    Beyreuther, K., Weber, K., Geisler, N. and Klemm, A., Proc. Nat. Acad. Sci. (1973) 70, 3570–3580.CrossRefGoogle Scholar
  2. 2.)
    Fowler, A.V. and Zabin, I., Proc. Nat. Acad. Sci. (1977) 74, 1507–1510.PubMedCrossRefGoogle Scholar
  3. 3.)
    Farabaugh, P.J., Nature (1978) 274, 765–769.PubMedCrossRefGoogle Scholar
  4. 4.)
    Buchel, D.E., Gronenborn, B. and Muller-Hill, B., Nature (1980) 283, 541–545.PubMedCrossRefGoogle Scholar
  5. 5.)
    Ehring, R., Beyrether, K., Wright, J.K. and Overath, P., Nature (1980) 283, 537–540.PubMedCrossRefGoogle Scholar
  6. 6.)
    Zabin, I. and Fowler, A.V., in The Operon, J.A. Miller and W.S. Reznikoff (Eds.), Cold Spring Harbor Laboratory, New York (1978) pp. 89–121.Google Scholar
  7. 7.)
    Brown, J.L., Koorajian, S. and Zabin, I., J. Biol. Chem. (1967) 242, 4259–4262.PubMedGoogle Scholar
  8. 8.)
    Reznikoff, W.S., Winter, R.B. and Hurley, C.K., Proc. Nat. Acad. Sci. (1974) 71, 2314–2318.PubMedCrossRefGoogle Scholar
  9. 9.)
    Gilbert, W., Gralla, J., Majors, J. and Maxam, A., in Protein-Ligand Interactions, (Ed. H. Sund and G. Blauer ), Walter de Gruyter and Co., Berlin (1975) pp. 193–210.Google Scholar
  10. 10.)
    Calos, M.P. and Miller, J.H., Nature (1980) 285, 38–41.PubMedCrossRefGoogle Scholar
  11. 11.)
    Hood, J.M., Fowler, A.V. and Zabin, I., Proc. Nat. Acad. Sci. (1978) 75, 113–116.PubMedCrossRefGoogle Scholar
  12. 12.)
    Katinka, M. et al., Proc. Nat. Acad. Sci. (1980) 77, 5730–5733.PubMedCrossRefGoogle Scholar
  13. 13.)
    Ullmann, A., Jacob, F. and Monod, J., J. Mol. Biol. (1967) 24, 339–343.PubMedCrossRefGoogle Scholar
  14. 14.)
    Fowler, A.V. and Zabin, I., J. Mol. Biol. (1968) 33, 35–47.PubMedCrossRefGoogle Scholar
  15. 15.)
    Grodzicker, T. and Zipser, D., J. Mol. Biol. (1968) 38, 305–314.PubMedCrossRefGoogle Scholar
  16. 16.)
    Newton, A., J. Mol. Biol. (1978) 125, 449–466.CrossRefGoogle Scholar
  17. 17.)
    Michels, C. and Zipser, D., J. Mol. Biol. (1969) 41, 341–347.PubMedCrossRefGoogle Scholar
  18. 18.)
    Manley, J.L., J. Mol. Biol. (1978) 125, 449–466.PubMedCrossRefGoogle Scholar
  19. 19.)
    Ullmann, A., Perrin, D., Jacob, F. and Monod, J., J. Mol. Biol. (1965) 12, 918–923.PubMedCrossRefGoogle Scholar
  20. 20.)
    Goldberg, M.E. (1970) The lactose operon (ed. J.R. Beckwith and D. Zipser), pp. 273–278, Cold Spring Harbor, New York.Google Scholar
  21. 21.)
    Celada, F., Ullmann, A. and Monod, J., Biochemistry (1974) 13, 5543–5547.PubMedCrossRefGoogle Scholar
  22. 22.)
    Raibaud, O., Clements, J.M., and Hofnung, M., Molec. Gen. Genet. (1979) 174, 261–267.PubMedCrossRefGoogle Scholar
  23. 23.)
    Bedouelle, H., Bassford, P.J., Jr., Fowler, A.V., Zabin, I., Beckwith, J. and Hofnung, M., Nature (1980) 285, 78–81.PubMedCrossRefGoogle Scholar
  24. 24.)
    Moreno, F., Fowler, A.V., Hall, M., Silhavy, T.J., Zabin, I. and Schwartz, M., Nature (1980) 286, 356–359.PubMedCrossRefGoogle Scholar

Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • Audree V. Fowler
    • 1
  • Irving Zabin
    • 1
  1. 1.Department of Biological Chemistry, UCLA School of Medicine and Molecular Biology InstituteUniversity of CaliforniaLos AngelesUSA

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