Manual Batchwise Sequencing Methods

Abstract

Until recently the rate-limiting step in protein structural determination was the purification of peptides, so sequencing efforts focused on long degradations of large fragments. With the advent of HPLC methods the sequencer has been confronted with a host of suitable material mostly of small to moderate size. Improvements in procedure for automatic instruments like the spinning cup have extended their applicability, range and sensitivity, but have only slightly increased their speed: one day’s worth of HPLC runs can easily produce enough peptides to occupy an automatic sequenator for half a year.