Biochemical and Genetic Studies on the Initial Events of Fruitbody Formation
The process of fruitbody formation in higher basidiomycetes involves the expression of a series of structural and regulatory genes required for the morphogenetic reactions. The first reaction necessary to initiate fruiting may be triggered by a genetic factor under certain environmental conditions. The incompatibility factors have been studied extensively since it has been shown that these factors regulate dikaryotization prerequisite to fruiting in the normal process of development (Raper, 1966; Raper and Raper, 1968). It is, however, known that fruiting is not limited to dikaryotic mycelia; monokaryotic mycelia produce fruitbodies under particular conditions such as aging, injury, or influence of some substances (Stahl and Esser, 1976). Various types of mutants that form fruitbodies on monokaryotic mycelia have also been found (Stahl and Esser, 1976). Study of monokaryotic fruiting has the advantage of dealing with a simple system of initiating fruiting free from the combination of incompatibility factors. We have made a series of experiments showing that adenosine 3′,5′-cyclic monophosphate (cyclic AMP) is one of the trigger substances in monokaryotic fruiting of Coprinus macrorhizus Rea f. microsporus Hongo. The present report describes the outline of the work and suggests a possible mechanism through which cyclic AMP exerts the effect on fruiting.
KeywordsGlycogen Phosphorylase Fruiting Body Formation Mycelial Extract Mycelial Cell Glycogen Phosphorylase Activity
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