Chemical Studies on the Porcine Myelin Basic Protein

  • Chi-Ling Wang
  • Wen-Chang Chang
Part of the Experimental and Clinical Neuroscience book series (ECN)


An encephalitogenic myelin basic protein was isolated from fresh frozen pig brains by acid- acetone extraction and purified to homogeneity by CM-ceilulose column chromatography and gelfiltration on a Sephacryl S-200 column. In addition to its ability to induce experimental encephalomyelitis in guinea pigs, this protein exhibited the characteristic properties of mammalian myelin basic proteins: high content of glycine and basic amino acid residues: presence of methylated arginine residues and absence of cystine or cysteine: absence of a-helix as revealed by circular dichroic studies in aqueous solution and high isoelectric point. The molecular weight as determined by SDS-polyacrylamidegel electrophoresis was around 20,000, in contrast to 36,000 obtained by gel-filtration on a Sepnadex G-100 column. The latter value suggests that the protein might exist as dimers in aqueous solution.


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  1. Brostoff SW, Reuter, W, Hichens, M and Eylar, EH (1974) Specific cleavage of the Ai protein from myelin with cathepsin D. J. Biol. Chem. 249, 559–567PubMedGoogle Scholar
  2. Carnegie, PR (1971) Amino acid sequence of the eneephalitogenic basic protein from human myelin., Biochem. J. 123, 57–67PubMedGoogle Scholar
  3. Deibler, GE and Martenson, RE (1973) Determination of methylated basic amino acids with the amino acid analyzer. Application to total acid hydrolyzates of myelin basic proteins., J. Biol. Chem. 248, 2387–2391.PubMedGoogle Scholar
  4. Dunkley, PR and Carnegie, PR (1974) Amino acid sequence of the smaller basic protein from rat brain myelin., Biochem. J. 141, 243–255PubMedGoogle Scholar
  5. Eylar, EH, Brostoff, S, Hashim, G, Caccam, J and Burnett, P (1971) Basic A-protein of the myelin membrane., The complete amino acid sequence., J. Biol. Chem. 246, 5770–5784PubMedGoogle Scholar
  6. Eylar, EH, Kniskern, PJ and Jackson, JJ (1974) Myelin basic proteins., In: Methods in Enzymology ( Fleischer, S and Packer, L, eds.) Vol. 32, pp. 323–341, Academic Press, New YorkGoogle Scholar
  7. Li, CH, Danho, WO, Chung, D and Rao, AJ (1975) Isolation, characterization, and amino acid sequence of melanotropins from camel pituitary glands. Biochemistry 14, 947–952.PubMedCrossRefGoogle Scholar
  8. Martenson, RE, Deibler, GE, Kies, MW, McKneally, SS, Shapira, R and Kibler, RF (1972) Differences between the two myelin basic proteins of the rat central nervous system., A deletion in the smaller protein., Biochim. Biophys. Acta 263, 193–203.PubMedGoogle Scholar
  9. Martenson, RE (1981) Prediction of the secondary structure of myelin basic protein., J. Neurochem. 36, 1543–1560.PubMedCrossRefGoogle Scholar
  10. Neville, Jr, DM (1971) Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J. Biol. Chem. 246, 6328–6334.PubMedGoogle Scholar
  11. Reisreid, RA, Lewis, UJ and Williams, DE (1962) Disk electrophoresis of basic proteins and peptides on polyacrylamide gels., Nature 195, 281–283CrossRefGoogle Scholar
  12. Spackman, DH, Stein, WH and Moore, S (1958) Automatic recording apparatus for use in the chromatography of amino acids., Anal. Chem. 30, 1190–1206.CrossRefGoogle Scholar
  13. Swann, RW and Li, CH (1979) Characterization of turkey myelin basic protein isolated by a simple procedure. Int. J. Peptide Protein Res. 14, 495–503.CrossRefGoogle Scholar
  14. Tomasi, LG and Kornguth, SE (1967) Purification and partial characterization of a basic protein from pig brain., J. Biol. Chem. 242, 4933–4938PubMedGoogle Scholar
  15. Tomasi, LG and Kornguth, SE (1968) Characterization and immunochemical localization of a basic protein from pig brain., J. Biol. Chem. 243, 2507–2513PubMedGoogle Scholar

Copyright information

© The Humana Press Inc. 1983

Authors and Affiliations

  • Chi-Ling Wang
    • 1
  • Wen-Chang Chang
    • 1
  1. 1.Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical SciencesNational Taiwan UniversityTaipeiTaiwan China

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