H-2-Linked IR Gene Control of H-2 Unrestricted Cytotoxic T Lymphocytes
The ability to generate H-2 unrestricted cytotoxic T lymphocytes (CTL) in vitro to the Qa-1b alloantigen following in vivo priming is under immune response (IR) gene control. B6.Tlaa mice lack the dominant IR gene and therefore cannot generate anti-Qa-1b CTL when immunised with Qa-1 congenic cells from B6 mice. A and (B6.Tla x A)F1 animals do respond to Qa-1 when immunized with congenic A.Tlab or (B6 x A) F1 cells, respectively. Segregation analysis indicates that the ability to respond is linked to the H-2 gene complex. The lack of the IR gene(s) does not represent a CTL repertoire defect because anti-Qa-1 CTL precursors (CTL.P) exist in the B6.Tla mice and can be activated if Qa-1b is presented together with helper determinants during the in vivo priming. Recognition of helper determinants need not occur during in vitro culture. Therefore, an H-2 linked IR gene controls the ability of animals to recognize helper determinants in vivo, a process requisite for the generation of CTL from unprimed CTL precursors.
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