Abstract
The unmatched affinity of avidin for binding to biotin (1) provides a unique tool for tracing and characterizing the structure of the avidin molecule during cellular processing and presentation. Accordingly, we have undertaken studies using avidin as an antigen, the results of which support the following conclusions
-
1.
Ir genes regulating T cell immune responses to avidin map to I-A and are Reflected in marked differences between high (I-A s, b, d) and low responders (I-Ak) (Table 1).
-
2.
After uptake and processing by macrophages avidin becomes 1000-fold more potent in stimulating proliferation of anti-avidin T lymphocytes.
-
3.
The molecular differences between processed avidin and native avidin involve breakdown into monomeric or smaller fragments and changes in sugar moieties. Avidin degraded to the point at which it will no longer bind biotin will not activate T lymphocytes. Thus, conservation of three-dimensional integrity of the molecule is correlated ith its antigenicity for anti-avidin T lymphocytes.
-
4.
Processed avidin is reversibly complexed with an H-2 restriction moiety originating from the macrophages. The complex itself can activate anti-avidin lines of T lymphocytes free of antigen-presenting cells. Such lines, without added macrophages, will not respond to native avidin or to processed avidin that is not complexed. Avidin primed lymph node cells, in contrast, will respond to processed avidin that is not complexed to the H-2 restriction element.
-
5.
Macrophages from Ir gene high or low responder mice are indistinguishable in their up-take and processing of avidin. The differences in Ir phenotype appear to be related to the population of T lymphocytes to which the processed antigen is addressed by its complexing with the restriction moiety.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Green, N.M. (1975). Adv. Prot. Chem. 21, 85–133.
Bolton, A.E. and Hunter, W.M. (1973). Biochem. J. 133, 529–539.
Ben-Nun, A. and Cohen, I.R. (1982). J. Immunol. 129, 303–308.
Eshhar, Z., et al., (1979). Cell. Immunol. 47. 378–389.
Titus, R.G. and Chiller, J.M. (1981). J. Immunol. Meth. 45., 65–78.
Unanue, E.R., Cerottini, J-C., and Bedford, N. (1969). Nature, 222, 1193–1195.
Ziegler, K. and Unanue, E.R. (1931). J. Immunol. 127. 1869–1875.
Erb, P. and Feldman, M. (1975). Eur. J. Immunol, 5, 759–766.
Puri, J. and Lonai, P. (1980). Eur. J. Immunol. 10, 2723–281.
Cohen, I.R., et al., (1979). Proc. Natl. Acad. Sci. USA., 76, 4066–4070.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 The Humana Press Inc.
About this chapter
Cite this chapter
Friedman, A., Gitler, C., Cohen, I.R. (1983). Molecular Aspects of the Processing and Presentation of Avidin by Macrophages to T Lymphocytes. In: Pierce, C.W., Cullen, S.E., Kapp, J.A., Schwartz, B.D., Shreffler, D.C. (eds) Ir Genes. Experimental Biology and Medicine, vol 4. Humana Press. https://doi.org/10.1007/978-1-4612-5633-5_40
Download citation
DOI: https://doi.org/10.1007/978-1-4612-5633-5_40
Publisher Name: Humana Press
Print ISBN: 978-1-4612-5635-9
Online ISBN: 978-1-4612-5633-5
eBook Packages: Springer Book Archive