The Use of Enzymatic Radioisotopic Microassays for the Quantification of β-Phenylethylamine, Phenylethanolamine, Tyramine and Octopamine

  • Juan M. Saavedra


Enzymatic radioisotopic microassays have been used to quantitate those biogenic amines present in mammalian brain in small amounts. Methods were developed for β-phenylethylamine, phenylethanolamine, octopamine and tyramine. The use of partially purified and group-specific N-methyl and 0-methyltransferases, combined with selective procedures for the complete separation of the reaction products, including chromatographic steps, resulted in specific and sensitive microassays. Similar procedures are used for the determination of the activities of enzymes which synthesize or metabolize these amines.

The use of such methods allows the study of levels and metabolism of amines in discrete areas of mammalian brain, plasma, and in single cells from invertebrates, where these amines might play a role as neurotransmitters.


Biogenic Amine Borate Buffer Isoamyl Alcohol Methyl Acetate Chromaffin Granule 
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  1. Axelrod, J. (1962) Purification and properties of phenyl- ethanolamine N- methyl transferase. J. Biol. Chem. 237, 1657–1660.PubMedGoogle Scholar
  2. Danielson, T.J., Boulton, A.A., Robertson, H.A. (1977) m-Octopamine, p-octopamine and phenylethanolamine in rat brain: A sensitive, specific assay and the effects of some drugs. J. Neurochem. 29, 1131–1135.PubMedCrossRefGoogle Scholar
  3. Molinoff, P.B., Landsberg, L. and Axelrod, J. (1969) An enzymatic assay for octopamine and other e-hydroxylat- ed phenyl ethyl amines. J. Pharm. Exp. Ther. 170, 253–261.Google Scholar
  4. Molinoff, P.B., Weinshilbourn, R. and Axelrod, J. (1971) A sensitive enzymatic assay for dopamine-e-hydroxylase. J. Pharm. Exp. Ther. 178, 425–431.Google Scholar
  5. Saavedra, J.M. (1974a) Enzymatic isotopic assay for and presence of e-phenylethyl amine in brain. J. Neurochem. 22, 211–216.PubMedCrossRefGoogle Scholar
  6. Saavedra, J.M. (1974b) Enzymatic-isotopic method for octopamine at the picogram level. Anal. Biochem. 59, 628–633.PubMedCrossRefGoogle Scholar
  7. Saavedra, J.M. (1979) Microquantitation of neurotransmitters in specific areas of the central nervous system. Int. Rev. Neurobiol. 21, 259–274.PubMedCrossRefGoogle Scholar
  8. Saavedra, J.M. and Axelrod, J. (1973T Demonstration and distribution of phenylethanolamine in brain and other tissues. Proc. Natl. Acad. Sci. USA 70, 769–772.Google Scholar
  9. Saavedra, J.M., Palkovits, M., Brownstein, M.J. and Axelrod, J. (1974) Localisation of phenylethanolamine N-methyl- transferase in the rat brain nuclei. Nature 248, 695–696.PubMedCrossRefGoogle Scholar
  10. Smith, A.D. and Winkler, H. (1967) A simple method for the isolation of adrenal chromaffin granules on a large scale. Biochem. J. 103, 480–482.PubMedGoogle Scholar
  11. Tallman, J.F., Saavedra, J.M. and Axelrod, J. (1976) A sensitive enzymatic-isotopic method for the analysis of tyramine in brain and other tissues. J. Neurochem. 27, 465–469.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1984

Authors and Affiliations

  • Juan M. Saavedra
    • 1
  1. 1.Section on PharmacologyLCS, NIMHBethesdaUSA

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