The Effect of Stress on Central Nervous System Protein Phosphorylation and Cyclic Amp

  • Peter R. Dunkley
  • Jill Cockburn
  • Peter A. Power
  • Maurice G. King
Part of the Experimental and Clinical Neuroscience book series (ECN)


The effect of mild stressors on central nervous (CNS) levels of protein phosphorylation and cyclic AMP was assessed in rats. Uncontrollable electric footshock significantly increased the in vitro phosphorylation of a protein of approximate molecular weight 42,000 that had been identified previously as the α subunit of pyruvate dehydrogenase. This increase was not due to the stress of handling the animal before the experiment or during the sacrifice procedures, but may have been due in part to exposure to a novel environment. No other phosphoprotein in the crude synaptosome fraction was significantly affected. Cyclic AMP levels were also unchanged. The literature is discussed with special reference to the procedures used to estimate in vivo levels of protein phosphorylation and cyclic AMP.


Microwave Irradiation Protein Phosphorylation Pyruvate Dehydrogenase Electric Footshock Uncontrollable Stress 
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Copyright information

© The Humana Press Inc. 1983

Authors and Affiliations

  • Peter R. Dunkley
    • 1
  • Jill Cockburn
    • 1
  • Peter A. Power
    • 2
  • Maurice G. King
    • 2
  1. 1.The Neuroscience Group, Medical Faculty and Psychology DepartmentUniversity of NewcastleAustralia
  2. 2.Psychology DepartmentUniversity of NewcastleAustralia

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