Abstract
The effect of mild stressors on central nervous (CNS) levels of protein phosphorylation and cyclic AMP was assessed in rats. Uncontrollable electric footshock significantly increased the in vitro phosphorylation of a protein of approximate molecular weight 42,000 that had been identified previously as the α subunit of pyruvate dehydrogenase. This increase was not due to the stress of handling the animal before the experiment or during the sacrifice procedures, but may have been due in part to exposure to a novel environment. No other phosphoprotein in the crude synaptosome fraction was significantly affected. Cyclic AMP levels were also unchanged. The literature is discussed with special reference to the procedures used to estimate in vivo levels of protein phosphorylation and cyclic AMP.
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Dunkley, P.R., Cockburn, J., Power, P.A., King, M.G. (1983). The Effect of Stress on Central Nervous System Protein Phosphorylation and Cyclic Amp. In: Kidman, A.D., Tomkins, J.K., Morris, C.A., Cooper, N.A. (eds) Molecular Pathology of Nerve and Muscle. Experimental and Clinical Neuroscience. Humana Press. https://doi.org/10.1007/978-1-4612-5308-2_21
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