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Methods: X-Ray Crystallography, Potential Energy Calculations, and Spectroscopy

  • Wolfram Saenger
Part of the Springer Advanced Texts in Chemistry book series (SATC)

Abstract

Nucleoside, nucleotide and nucleic acid structures have been elucidated mainly by X-ray crystallographic techniques. These methods provide information at different levels of detail depending on the molecular weight and organization of the material into crystalline or quasi-crystalline matrices. Readers not familiar with X-ray diffraction methods should be aware of the basic principles; they will then understand why the results of X-ray crystal structure analyses of small molecules up to the oligonucleotide level are unambiguous and why those of crystalline macromolecules like tRNA can, in some close details, be a matter of interpretation. Fiber diffraction analysis of quasi-crystalline polymers like DNA and RNA gives only approximate, overall structural information and cannot yield a satisfying structural model without additional data obtained by other methods.

Keywords

Crystal Structure Analysis Structure Amplitude Nucleic Acid Structure Pitch Height Backbone Torsion Angle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1984

Authors and Affiliations

  • Wolfram Saenger
    • 1
  1. 1.Institut für KristallographieFreie UniversitätFederal Republic of Germany

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