Coordinate and Non-Coordinate Regulation of Histone mRNAs during Myoblast Growth and Differentiation

  • R. Curtis Bird
  • Fred A. Jacobs
  • Bruce H. Sells
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 5)

Abstract

The fusion of myoblasts to produce non-dividing syncytial myotubes marks the end of cell proliferation during muscle cell differentiation. Following the signal to differentiate myoblasts enter G1 phase, retire from the cell cycle and differentiate into myotubes (3,4). The synthesis of muscle specific proteins is initiated as the cells begin to differentiate and defines the onset of the differentiated state (2,7). However the conditions necessary and sufficient to allow a proliferating population of cells to withdraw from the cell cycle and to switch from a growth specific to a differentiation specific program of gene expression have yet to be defined.

Keywords

Polyacrylamide Stein Thymidine Hydroxyurea Actinomycin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bird, R.C., F. A. Jacobs, G. Stein, J. Stein, and B. H. Sells. 1984. Coordinate regulation of histone mRNAs during growth and differentiation of rat myoblasts. In preparation.Google Scholar
  2. 2.
    Caravatti, M., A. Minty, B. Robert, D. Montarras, A. Weydert, A. Cohen, P. Daubas, and M. Buckingham. 1982. Regulation of muscle gene expression. The accumulation of messenger RNAs coding for muscle-specific proteins during myogenesis in a mouse cell line. J. Mol. Biol. 160: 59–76.PubMedCrossRefGoogle Scholar
  3. 3.
    Holtzer, H., G. Yeoh, N. Rubenstein, J. Chi, S. Fellini, and S. Dienstman. 1977. A review of controversial issues in myogenesis. In Regulation of Cell Proliferation and Differentiation. Plenum Press, NY. 87–104.Google Scholar
  4. 4.
    Nadal-Ginard, B. 1978. Commitment, fusion and biochemical differentiation of a myogenic cell line in the absence of DNA synthesis. Cell 15: 855–864.PubMedCrossRefGoogle Scholar
  5. 5.
    Plumb, M., J. Stein, and G. S. Stein. 1983. Coordinate regulation of multiple histone mRNAs during the cell cycle in HeLa cells. Nucl. Acids Res. 11: 2391–2410.PubMedCrossRefGoogle Scholar
  6. 6.
    Ruzdijic, S.D., R.C. Bird, F.A. Jacobs, and B.H. Sells. 1984. Specific mRNP particles: Characterization of the proteins bound to histone H4 mRNAs isolated from L6 myoblasts. In preparation.Google Scholar
  7. 7.
    Shani, M., D. Zevin-Sonkin, O. Saxel, Y. Carmon, D. Katcoff, U. Nudel, and D. Yaffe. 1981. The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of corresponding mRNA sequences during myogenesis. Develop. Biol. 86: 483–492.PubMedCrossRefGoogle Scholar
  8. 8.
    Stein, G.S., and T.W. Borun. 1972. The synthesis of acidic chromosomal proteins during the cell cycle of HeLa S3 cells. I. J. Cell Biol. 52: 292–307.PubMedCrossRefGoogle Scholar
  9. 9.
    Wu, R.S., and W. M. Bonner. 1981. Separation of basal histone synthesis from S-phase histone synthesis in dividing cells. Cell 27: 321–330.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1984

Authors and Affiliations

  • R. Curtis Bird
    • 1
  • Fred A. Jacobs
    • 1
  • Bruce H. Sells
    • 1
  1. 1.Department of Molecular Biology and Genetics, College of Biological ScienceUniversity of GuelphGuelphCanada

Personalised recommendations