Gastrointestinal Cells: Growth Factors, Transformation, and Malignancy

  • M. P. Moyer
  • P. Dixon
  • D. Escobar
  • J. B. Aust
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 5)

Abstract

At the cellular level, growth regulation and differentiation of normal and malignant gastrointestinal (GI) epithelium are poorly understood. This is due to many factors, including previous difficulties in culturing GI cells in vitro for a useful time period (1–4). That problem has been rectified by our development of alternative methods for culturing normal human and animal GI cells (5,6) and GI tumor cells (7). In addition, our recent successful transformation of human colon cells (8) with the chemical carcinogen, azoxymethane, and the oncogenic virus simian virus (SV40) provides a baseline for expanding studies of in vitro transformation to include alternate sources of GI cells. In this paper, we relate some recent studies of in vitro transformation, growth, and characterization utilizing human and rodent GI cells.

Keywords

Agar Hydrocortisone Selenium Sarcoma Dexamethasone 

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Copyright information

© The Humana Press Inc. 1984

Authors and Affiliations

  • M. P. Moyer
    • 1
  • P. Dixon
    • 1
  • D. Escobar
    • 1
  • J. B. Aust
    • 1
  1. 1.The University of Texas Health Science Center at San AntonioSan AntonioUSA

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