Gastrointestinal Cells: Growth Factors, Transformation, and Malignancy
At the cellular level, growth regulation and differentiation of normal and malignant gastrointestinal (GI) epithelium are poorly understood. This is due to many factors, including previous difficulties in culturing GI cells in vitro for a useful time period (1–4). That problem has been rectified by our development of alternative methods for culturing normal human and animal GI cells (5,6) and GI tumor cells (7). In addition, our recent successful transformation of human colon cells (8) with the chemical carcinogen, azoxymethane, and the oncogenic virus simian virus (SV40) provides a baseline for expanding studies of in vitro transformation to include alternate sources of GI cells. In this paper, we relate some recent studies of in vitro transformation, growth, and characterization utilizing human and rodent GI cells.
KeywordsEpithelial Cell Culture Rodent Cell Transepithelial Transport Dome Formation Human Colon Cell
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